scholarly journals In vivo determination of body composition by tritium dilution in the rat

1979 ◽  
Vol 41 (3) ◽  
pp. 625-628 ◽  
Author(s):  
Nancy J. Rothwell ◽  
M. J. Stock
Keyword(s):  
Body Water ◽  
Female Rats ◽  
Indirect Methods ◽  
Male And Female ◽  
Male And Female Rats ◽  
Significant Difference ◽  
Total Body ◽  
Direct And Indirect Methods

Total body water was determined in vivo by tritium dilution in thirty-two male and female rats. Body water obtained by tritium dilution and body fat calculated from this value correlated significantly with body water and fat obtained by analysis (r 0.985, 0.855 respectively). There was no significant difference between values for fat assessed by the direct and indirect methods.

Effect of Chronic Lead Exposure on Kidney Function in Male and Female Rats: Determination of a Lead Exposure Biomarker

2001 ◽  
Vol 109 (5) ◽  
pp. 457-463 ◽  
Author(s):  
F. Ghorbe ◽  
M. Boujelbene ◽  
F. Makni-Ayadi ◽  
F. Guermazi ◽  
A. Kammoun ◽  
...  
Keyword(s):  
Kidney Function ◽  
Lead Exposure ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats ◽  
Exposure Biomarker ◽  
Chronic Lead Exposure

Role of estrogens and age in flow-mediated outward remodeling of rat mesenteric resistance arteries

2014 ◽  
Vol 307 (4) ◽  
pp. H504-H514 ◽  
Author(s):  
K. Tarhouni ◽  
M. L. Freidja ◽  
A. L. Guihot ◽  
E. Vessieres ◽  
L. Grimaud ◽  
...  
Keyword(s):  
Blood Flow ◽  
Female Rats ◽  
Male Rats ◽  
Resistance Arteries ◽  
Cross Sectional ◽  
Male And Female ◽  
Male And Female Rats ◽  
Arterial Contractility

In resistance arteries, a chronic increase in blood flow induces hypertrophic outward remodeling. This flow-mediated remodeling (FMR) is absent in male rats aged 10 mo and more. As FMR depends on estrogens in 3-mo-old female rats, we hypothesized that it might be preserved in 12-mo-old female rats. Blood flow was increased in vivo in mesenteric resistance arteries after ligation of the side arteries in 3- and 12-mo-old male and female rats. After 2 wk, high-flow (HF) and normal-flow (NF) arteries were isolated for in vitro analysis. Arterial diameter and cross-sectional area increased in HF arteries compared with NF arteries in 3-mo-old male and female rats. In 12-mo-old rats, diameter increased only in female rats. Endothelial nitric oxide synthase expression and endothelium-mediated relaxation were higher in HF arteries than in NF arteries in all groups. ERK1/2 phosphorylation, NADPH oxidase subunit expression levels, and arterial contractility to KCl and to phenylephrine were greater in HF vessels than in NF vessels in 12-mo-old male rats only. Ovariectomy in 12-mo-old female rats induced a similar pattern with an increased contractility without diameter increase in HF arteries. Treatment of 12-mo-old male rats and ovariectomized female rats with hydralazine, the antioxidant tempol, or the angiotensin II type 1 receptor blocker candesartan restored HF remodeling and normalized arterial contractility in HF vessels. Thus, we found that FMR of resistance arteries remains efficient in 12-mo-old female rats compared with age-matched male rats. A balance between estrogens and vascular contractility might preserve FMR in mature female rats.

Comparative pulmonary toxicity of inhaled metalworking fluids in rats and mice

2016 ◽  
Vol 33 (5) ◽  
pp. 385-405 ◽  
Author(s):  
Kristen R Ryan ◽  
Mark F Cesta ◽  
Ronald Herbert ◽  
Amy Brix ◽  
Michelle Cora ◽  
...  
Keyword(s):  
Animal Studies ◽  
Clinical Chemistry ◽  
Female Rats ◽  
Metalworking Fluids ◽  
Whole Body ◽  
Chemical Components ◽  
Male And Female ◽  
Male And Female Rats ◽  
Rats And Mice

Metalworking fluids (MWFs) are complex formulations designed for effective lubricating, cooling, and cleaning tools and parts during machining operations. Adverse health effects such as respiratory symptoms, dermatitis, and cancer have been reported in workers exposed to MWFs. Several constituents of MWFs have been implicated in toxicity and have been removed from the formulations over the years. However, animal studies with newer MWFs demonstrate that they continue to pose a health risk. This investigation examines the hypothesis that unrecognized health hazards exist in currently marketed MWF formulations that are presumed to be safe based on hazard assessments of individual ingredients. In vivo 13-week inhalation studies were designed to characterize and compare the potential toxicity of four MWFs: Trim VX, Cimstar 3800, Trim SC210, and Syntilo 1023. Male and female Wistar Han rats or Fischer 344N/Tac rats and B6C3F1/N mice were exposed to MWFs via whole-body inhalation at concentrations of 0, 25, 50, 100, 200, or 400 mg/m3 for 13 weeks, after which, survival, body and organ weights, hematology and clinical chemistry, histopathology, and genotoxicity were assessed following exposure. Although high concentrations were used, survival was not affected and toxicity was primarily within the respiratory tract of male and female rats and mice. Minor variances in toxicity were attributed to differences among species as well as in the chemical components of each MWF. Pulmonary fibrosis was present only in rats and mice exposed to Trim VX. These data confirm that newer MWFs have the potential to cause respiratory toxicity in workers who are repeatedly exposed via inhalation.

scholarly journals Gastrin, Histamine Prostaglandin: Indicators for assessing efficacy of cimetidine, Omeprazole and Ranitidine in Gastric Ulcer Treatment

2018 ◽  
Vol 5 (2) ◽  
pp. 5-12
Author(s):  
Jimmy Etukudo Okon ◽  
Gideon Umezuruike Egesie
Keyword(s):  
Gastric Ulcer ◽  
High Performance ◽  
Female Rats ◽  
Albino Rats ◽  
Radical Cure ◽  
Male And Female ◽  
Ulcer Disease ◽  
Male And Female Rats ◽  
Significant Difference ◽  
Organ Specific

Background and Objectives: There is increase prevalence of gastric ulcer in the society, but the drugs that are sensitive for radical cure are not screened with physiologic markers such which affect proper management of the disease. The objective of the study is to relate various sources or organ specific templates: gastrin, histamine and prostaglandin relating with the disease in evaluating the potencies of cimetidine, ranitidine and omeprazole for best choice of the drugs in gastric ulcer disease treatment.Material and Methods: Plasma, gastric and antral prostaglandins, histamine and gastrin levels were studied in ninety-six (96), male and female Swiss albino rats for 28 days, using high performance liquid chromatography.Results: Male and female rats with gastric ulcer treated with cimetidine, omepraszole and ranitidine showed no significant difference (P>0.5) in gastrin and the drug groups in plasma, gastric and antral concentrations. But, there was significant difference (P<0.05) in histamine levels between cimetidine, omeprazole and ranitidine in their gastric and plasma concentration. There was no significant difference (P>0.05) in prostaglandin values between cimetidine, omeprazole and ranitidine. Also there was no significant difference (P>0.05) in gastric and plasma levels of gastrin, histamine and prostaglandin between 7, 14, 21 and 28 days treatment period. But, there was significant difference (P<0.05) in antral concentration of gastrin, histamines and prostaglandin between the drug groups. However, there was no significant difference (P>0.05) in antral gastrin between male and female rats in cimetidine and ranitidine treatment. The three drugs were associated with high levels of gastrin, histamine, low prostaglandin though cimetidine showed higher concentration of prostaglandin.Conclusion: It is concluded that gastrin, histamine and prostaglandin are sensitive indicators in evaluating anti-ulcerogenic drugs efficacies.Janaki Medical College Journal of Medical Sciences (2017) Vol. 5(2): 5-12

Abstract 269: Cardiac Aging in Rats: Do Female and Male Hearts Age Differently?

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Eduard I Dedkov ◽  
Alessandro Pingitore
Keyword(s):  
Experimental Studies ◽  
Left Ventricular ◽  
Female Rats ◽  
Male Rats ◽  
Laboratory Animals ◽  
Cardiac Aging ◽  
Male And Female ◽  
Male And Female Rats ◽  
Significant Difference ◽  
Systolic Performance

Background: In recent years, the use of middle-aged and old animals of both sexes has become preferential in experimental studies involving the models of cardiovascular diseases to better reflect a human population. However, the evidence showing the extent of sex-related differences in cardiac aging of laboratory animals remains obscure. Accordingly, we designed our study to determine whether the male and female rats differ during aging with regard to left ventricular (LV) geometry and systolic function. Methods: We performed transthoracic echocardiographic examinations in 12-month-old (12mo) and 24 month-old (24mo) male and female Sprague-Dawley rats (Charles River, Wilmington, MA) under 1.5% isoflurane anesthesia using a linear (Matrix) array transducer probe GE M12L (5.5-14.0 MHz) and a GE Vivid 7 Dimension ultrasound system. Two-dimensional short-axis views and M-mode tracings of the LV at the papillary muscle level were recorded and used to estimate various LV parameters. Results: We found that 24mo-Female rats had a significant increase in LV chamber dimensions associated with a reduction in its systolic performance as compared to 12mo-Female rats (Table 1). In contrast, LV parameters remained similar between 12mo- and 24mo-Male rats. Table 1. Echocardiographic parameters of LV chamber geometry and systolic performance Values are means ± SE. Arrow indicates a significant difference between age groups ( P ≤ 0.05). Conclusion: Our data demonstrate that during cardiac aging female rats have a different pattern of LV changes compared to males that must be considered in the design of future experimental studies.

Effects of corticotropin-releasing factor on energy balance in rats are sex dependent

1989 ◽  
Vol 257 (6) ◽  
pp. R1417-R1422 ◽  
Author(s):  
S. Rivest ◽  
Y. Deshaies ◽  
D. Richard
Keyword(s):  
Body Weight ◽  
Energy Balance ◽  
Food Intake ◽  
Serum Testosterone ◽  
Corticotropin Releasing Factor ◽  
Female Rats ◽  
Male Rats ◽  
Male And Female ◽  
Male And Female Rats ◽  
Significant Difference

The purpose of this study was to investigate the effects of a chronic intracerebroventricular administration of corticotropin-releasing factor (CRF) on energy balance of male and female rats. One week after their delivery to the laboratory, both male and female rats were divided into two groups. One group in each sex was treated with human/rat CRF, while another group was infused with the vehicle. Chronic administration of CRF was accomplished by means of miniosmotic pumps connected to a cannula that was stereotaxically directed into the third ventricle. Food intake and body weight were measured each day during the study. After 14 days of treatment, the rats were killed by decapitation. Energy, fat, and protein contents of the carcasses were quantified. Serum testosterone and estradiol were assayed in males and females, respectively. Administration of CRF significantly reduced body weight gain and food intake in male rats. No significant difference in those variables was observed between female rats treated with CRF and their controls infused with saline. Similarly, metabolizable energy intake and body energy gain were reduced in male rats infused with CRF, whereas no difference was observed between female animals treated with CRF and those infused with saline. In male rats, body fat and body protein contents were lower in CRF-treated than in saline-infused rats. In female rats, CRF did not affect body composition. Serum testosterone in male rats and serum estradiol in female animals were reduced after chronic infusion of CRF.(ABSTRACT TRUNCATED AT 250 WORDS)

Study on in vivo and in vitro metabolism of dimethylformamide in male and female rats

Toxicology ◽  
1984 ◽  
Vol 29 (3) ◽  
pp. 221-234 ◽  
Author(s):  
V. Scailteur ◽  
E. de Hoffmann ◽  
J.P. Buchet ◽  
R. Lauwerys
Keyword(s):  
In Vitro Metabolism ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats

Different effects of (CIS+TRANS) 1,3-dichloropropene in renal cortical slices derived from male and female rats

1999 ◽  
Vol 18 (2) ◽  
pp. 106-110
Author(s):  
Livia Secondin ◽  
Stefano Maso ◽  
Andrea Trevisan
Keyword(s):  
Reduced Glutathione ◽  
Organic Anion ◽  
Female Rats ◽  
Male Rats ◽  
Aminooxyacetic Acid ◽  
Male And Female ◽  
Male And Female Rats ◽  
Cortical Slices

1 Nephrotoxic effects of 1,3-dichloropropene (cis and trans isomers mixture) was investigated in vitro by means of renal cortical slice model in male and female rats, including treatment with metabolism modifiers as an inducer of cytochrome P-450 1A class (β-naphtho-flavone), a reduced glutathione depleting (DL-buthio-nine-[S, R]-sulfoximine), an inhibitor of g-glutamyltransferase (AT-125) and inhibitor of cysteine conjugate β-lyase (aminooxiacetic acid).2 Dose-dependent decrease of p-aminohippurate uptake was observed in male renal cortical slices. Only the high doses (3.0 and 4.0×10-4M) caused a significant loss of organic anion uptake in females.3 β-Naphthoflavone and α-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid (AT-125) partially, but significantly, reduced organic anion loss in males. In females, DL-buthionine-[S, R]-sulfoximine significantly increased in females but in males loss of organic anion accumulation caused by 1,3-dichloropropene. Aminooxyacetic acid did not ameliorate 1,3 D effects in vivo and in vitro in male rats. It appeared very toxic for female rats (all rats died) after in vivo injection.4 Sensitivity to nephrotoxicity induced by 1,3-dichlor-opropene in vitro was about double in male than female rats. Reduced glutathione conjugation appeared involved in nephrotoxicity induced in males but in females, probably by means of a chloropropylcysteinylglycine-conjugate formation; slight toxicity in females is likely related to oxidative metabolism.

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