scholarly journals Differences in the metabolism of esterified and unesterified linoleic acid by rumen micro-organisms

1969 ◽  
Vol 23 (4) ◽  
pp. 869-878 ◽  
Author(s):  
J. H. Moore ◽  
R. C. Noble ◽  
W. Steele ◽  
J. W. Czerkawski
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Octadecenoic Acid ◽  
Time Intervals ◽  
Micro Organisms ◽  
Hydrolysis Of ◽  
Maize Oil

1. Sheep were given intraruminal infusions of maize oil or linoleic acid and samples of contents were taken from the rumen and abomasum at different times after the infusions. Hydrolysis of the maize oil occurred in the rumen with the production of mono- and di-glycerides as intermediates. Linoleic acid derived from the maize oil was hydrogenated to stearic acid. When linoleic acid was infused into the rumen, little or no stearic acid was produced and octadecenoic acid accumulated.2. When linoleic acid or maize oil was incubated with rumen contents in an artificial rumen and samples of the reaction mixtures were taken from the apparatus after various time intervals, the results were similar to those obtained in vivo, except that the hydrolysis of maize oil did not give rise to mono- and di-glycerides.3. These results are discussed in relation to previous findings on the effects of intraruminal infusions of maize oil or linoleic acid on the fatty acid composition of the blood triglycerides of sheep.

scholarly journals The effect of diet on the compositions of the triglycerides and unesterified fatty acids isolated from the plasma, liver and adipose tissues of rabbits

1966 ◽  
Vol 20 (1) ◽  
pp. 79-93 ◽  
Author(s):  
J. H. Moore ◽  
D. L. Williams
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Wheat Starch ◽  
Adipose Tissues ◽  
Plasma Triglycerides ◽  
Maize Oil

1. Groups of male rabbits (ten to thirteen per group survived) were given ad lib. a diet consisting of 80 parts of a low-fat basal diet to which were added: for group I, 20 parts maize oil; for group 2, 20 parts butterfat; for group 3, 10parts maize oil and and 10parts butter- fat; for group 4, 0.47 parts maize oil and 43.1 parts wheat starch; and for group 5, 10.2parts maize oil and 21.6 parts wheat starch. The animals in group 6 were givenan ordinary com- mercial rabbit diet. 2. The rabbits were given the various diets for 38 weeks, after which a sample of blood was taken. The rabbits were then killed and the liver and a sample of perine- phric adipose tissue were removed from each animal. The plasma, liver and adipose tissue lipids werefractionated on columns of Florisil and the fatty acid compositions of the tri- glyceride and unesterified fatty acid fractions were determined by gas-liquid chromatography.3. The effects of the different diets on the composition of the unesterifiedfatty acids in the plasma were very similar to the effects of the diets on the fatty acid composition of the plasma triglycerides, but in the plasma unesterified fatty acids the concentration of stearic acid was consistently higher and the concentration of linoleic acid was consistently lower than in the plasma triglycerides. 4. There appeared to be a positive rectilinear relationship between the concentration of stearic acid in the plasma triglycerides and the concentration of triglycerides in the plasma. 5. In the triglycerides of the plasma, the concentrations of palmitic and stearic acids were consistently greater and the concentration of linoleic acid was consistently less than the corresponding concentrations of these fatty acids in the triglycerides of the adipose tissues. In the two groups of rabbits given low-fat diets the fatty acid composition of the liver trigly- cerides was almost identical with that of the plasma triglycerides. 6. In each of the six groups of rabbits the composition of the unesterified fatty acids in the plasma was identical with that of the unesterified fatty acids in the adipose tissues. Inthe unesterified fatty acids of the liver the concentrations of linoleic andarachidonic acids were consistently greater and the concentrations of myristic, palmitic and palmitoleic acids were consistently less than the corresponding concentrations of these acids in the unesterified fatty acids of the plasma and adipose tissues. 7. The results of this investigation are discussedin the light of recent con-cepts on the metabolic relationships between the unesterified fatty acids and triglycerides of the liver, plasma and adipose tissues.

Depot fatty acids of Aberdeen Angus and Friesian cattle reared on hay and barley diets

1977 ◽  
Vol 89 (3) ◽  
pp. 575-582 ◽  
Author(s):  
W. M. F. Leat
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Stearic Acid ◽  
Acid Composition ◽  
Visual Inspection ◽  
Subcutaneous Fat ◽  
Octadecenoic Acid ◽  
Hexadecenoic Acid ◽  
Friesian Cattle

SummaryAberdeen Angus and Friesian cattle were reared from 4 months of age to slaughter weight at 18–24 months on either high-barley or high-hay diets. Samples of subcutaneous fat were taken by biopsy at 3 monthly intervals, and the degree of fatness of each animal was estimated ultrasonically prior to slaughter, and by visual inspection of the carcasses.The barley-fed animals gained weight more rapidly, and fattened more quickly than the hay-fed animals with the Angus being fatter than the Friesian at the same age. The percentage stearic acid (C18:0) in subcutaneous fat decreased with age and was replaced by octadecenoic acid (C18:l) and hexadecenoic acid (C16:l), these changes being more rapid in barley-fed than in hay-fed animals. At the same degree of fatness the depot fats of the Friesians were more unsaturated than those of the Angus, and in both breeds the fatter the animal the more unsaturated was its depot fat.In the hay-fed cattle the percentage C16:0 in subcutaneous fat increased during the last half of the experiment and at slaughter the percentage C16:0 was significantly higher, and C18:l significantly lower, in all depot fats compared with those of the barley-fed animals.It is concluded that the fatty acid composition of bovine depot fats is modulated by the degree of fattening, and can be affected by diet.

Oxidation of alpha 1-protease inhibitor: role of lipid peroxidation products

1989 ◽  
Vol 66 (5) ◽  
pp. 2211-2215 ◽  
Author(s):  
V. Mohsenin ◽  
J. L. Gee
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Protease Inhibitor ◽  
Alpha Tocopherol ◽  
Inhibitory Capacity ◽  
Buffer Control

Previously we demonstrated that in vivo exposure of humans to NO2 resulted in significant inactivation of alpha 1-protease inhibitor (alpha 1-PI) in the bronchoalveolar lavage fluid. However, alpha 1-PI retains its elastase inhibitory activity in vitro when exposed to 10 times the concentration of NO2 used in vivo. We suggested exogenous oxidants such as O2 and NO2 exert their effect in vivo in part through lipid peroxidation. We investigated the mechanism of inactivation of alpha 1-PI in the presence or absence of lipids under oxidant atmosphere. alpha 1-PI in solutions containing phosphate buffer (control), 0.1 mM stearic acid (saturated fatty acid, 18:0), or 0.1 mM linoleic acid (polyunsaturated fatty acid, 18:2) was exposed to either N2 or NO2 (50 ppm for 4 h). Elastase inhibitory capacity of alpha 1-PI was significantly diminished in the presence of 0.1 mM linoleic acid and under NO2 atmosphere (75 +/- 8% of control, P less than 0.01), whereas there was no change in elastase inhibitory capacity of alpha 1-PI in the presence or absence (buffer only) of 0.1 mM stearic acid under a similar condition (109 +/- 11 and 94 +/- 6%, respectively). The inactivated alpha 1-PI as the result of peroxidized lipid could be reactivated by dithiothreitol and methionine sulfoxide peptide reductase, suggesting oxidation of methionine residue at the elastase inhibitory site. Furthermore the inhibitory effect of peroxidized lipid on alpha 1-PI could be prevented by glutathione and glutathione peroxidase and to some extent by alpha-tocopherol.

Formaldehyde-treated casein–safflower oil supplement for dairy cows: II. Effect on the fatty-acid composition of plasma and milk lipids

1972 ◽  
Vol 39 (2) ◽  
pp. 211-218 ◽  
Author(s):  
L. J. Cook ◽  
T. W. Scott ◽  
Y. S. Pan
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Milk Fat ◽  
Plasma Lipids ◽  
Octadecenoic Acid ◽  
Safflower Oil ◽  
Milk Lipids ◽  
Micro Organisms ◽  
Fatty Acid Compositions

SummaryThis study reports the effect of feeding formaldehyde-treated or untreated spray-dried casein–safflower oil (1:1, w/w) particles on the fatty-acid composition of milk and plasma lipids of cows.When the formaldehyde-treated particles were given to Jersey, Sahiwal and Jersey × Sahiwal crossbred cows (1 kg per cow per day) the linoleic acid (18:2) present in the safflower oil was not hydrogenated by the rumen micro-organisms and was incorporated into milk fat.When the untreated supplement was fed, however, the 18:2 fatty acid was hydrogenated in the rumen and there was an increased proportion of octadecenoic acid (18:1) in the milk fat.The increased proportions of 18:2 (treated supplement) and 18:1 (untreated supplement) were associated with decreased proportions of palmitic (16:0) and myristic (14:0) acids in the milk fat. All 3 breeds showed similar responses.The fatty-acid composition of plasma triglycerides, which are an important source of long-chain fatty acids for mammary-gland lipogenesis, was similarly affected. Furthermore, there were significant changes in the fatty-acid composition of other plasma lipids (e.g. phospholipids and cholesteryl esters). The interrelationships between dietary, plasma and milk fatty-acid compositions are discussed.

scholarly journals The effect of dietary fats on the composition of the liver endoplasmic reticulum and oxidative drug metabolism

1979 ◽  
Vol 41 (3) ◽  
pp. 465-475 ◽  
Author(s):  
Catherine T. Hammer ◽  
E. D. Wills
Keyword(s):  
Fatty Acids ◽  
Endoplasmic Reticulum ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Acid Composition ◽  
Unsaturated Fatty Acids ◽  
High Rate ◽  
Oxidative Demethylation ◽  
Maize Oil

1. The dependence of the rate of oxidative demethylation in the liver endoplasmic reticulum on the fatty acid composition of the endoplasmic reticulum has been studied by varying the lipid content of the diet.2. The rate of oxidative demethylation was markedly dependent on the percentage of linoleic acid (18:2) incorporated into the membrane. Feeding diets containing (g/kg) 100 coconut oil, 100 lard or 100 maize oil caused respectively the incorporation of 7.6, 10.3 and 25.1% linoleic acid (18:2) and a demethylation rate of 3.26, 3.15 and 5.03 nmol formaldehyde/min per mg protein. Feeding 100 g herring oil/kg diet caused incorporation of only 5.1% C18:2 but also 27.2% ωw3 unsaturated fatty acids, including 8.7% eicosapentaenoic acid (20:5) and 17.0% docosahexaenoic acid (22.6) and caused a very high rate of oxidative demethylation (6.53 nmol formaldehyde/min per mg protein).3. Destruction of the polyunsaturated fatty acids in herring oil by irradiation with 400 krad caused incorporation of a smaller quantity of ωw3 unsaturated acids into the endoplasmic reticulum and decreased the rate of oxidative demethylation (4.83 nmol formaldehyde/min per mg protein).4. The inductive effects of phenobarbitone on oxidative demethylation were partially dependent on changes in the fatty acid composition of the endoplasmic reticulum. Phenobarbitone (100 mg/kg) increased the percentage of C18:2 from 25.1 to 29.4% in rats given a maize-oil diet, increased the percentage of C20:5 from 8.7 to 10.3% in rats given a herring-oil diet and decreased the percentage of arachidonic acid (20:4) and C22:6 in rats given a lard, maize-oil, herring-oil or irradiated-herring-oil diet.5. Intraperitoneal α-tocopherol (50 mg/kg) increased the percentage of C20:4 from 11.1 to 13.1% in rats given a lard diet and from 5.9 to 7.3% in rats given a herring-oil diet.6. It is concluded that dietary C18:2 is an important factor in the regulation of the rate of oxidative demethylation in the liver endoplasmic reticulum but this may be replaced effectively by dietary C20:5 ω3 and C22:6 ω3 acids. Oxidative demethylation is regulated by changes in the fatty acid composition of the membranes of the liver endoplasmic reticulum.

scholarly journals Evaluating Pig Performance, Carcass Merit and Processed Pork Quality when Chestnuts and Acorns are fed to Duroc-Influenced Pig Genetics During Late Finishing

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
K. S. Burdick ◽  
T. R. Schnurbusch ◽  
J. L. Slaughter ◽  
Z. D. Callahan ◽  
B. R. Wiegand
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Acid Composition ◽  
Palmitoleic Acid ◽  
Carcass Characteristics ◽  
Fat Content ◽  
Carcass Quality

ObjectivesThe objective of this study was to determine the effects of feeding chestnuts and acorns on growth performance, carcass quality and further processed products of Duroc/Duroc crossbred finishing barrows.Materials and MethodsBarrows (n = 30) were individually housed in pens, blocked by body weight and randomly assigned to one of three treatments: control (n = 10), inclusion of acorns at 15% of the diet (n = 7), inclusion of chestnuts at 15% of the diet (n = 13). Pigs were fed ad libitum for 28 d prior to harvest. Feed refusal and individual pig weights were collected every 7 d and used to calculate average daily gain (ADG), gain-to-feed (G:F), and average daily feed intake (ADFi). Following harvest, carcass quality was determined by objective color (L*, a* and b*), fat composition and marbling scores. Fat samples were removed from four fat depots (backfat, seam, jowl, kidney and pelvic) and analyzed for fatty acid composition. Sample chops were removed between the 10th and 11th rib of the left side of each carcass and analyzed for fatty acid composition, moisture and fat content. Bellies were removed from the left side of each carcass, further processed into bacon slabs and analyzed for slice quality, fatty acid composition, moisture and fat content. Carcass characteristics and bacon quality were analyzed using GLM procedure of SAS. Growth performance and fatty acid composition were analyzed using MIXED procedure of SAS. Significance was determined at P-value < 0.05.ResultsNo differences were detected for ADG and ADFi across treatments (P > 0.05). Barrows fed chestnut diets had a greater G:F when compared to control (P < 0.05) or acorn fed barrows (P < 0.05). Dietary treatments did not impact (P > 0.05) carcass characteristics or carcass quality. Inclusion of chestnuts or acorns within the diet did not impact (P > 0.05) moisture and fat content of chops and bacon slices (P > 0.05). Moreover, feeding acorns led to similar concentrations (P > 0.05) of palmitoleic acid (16:1) and linoleic acid (18:2n6c) when compared to the control diet. However, feeding diets containing acorns led to greater proportions (P < 0.01) of palmitoleic acid and linoleic acid similar to barrows fed diets containing chestnuts. No difference (P > 0.05) for stearic acid (18:0) were observed between control and chestnut treatments, however, both were found to have greater amounts (P < 0.01) of stearic acid when compared to the acorn treatment. Acorns increased (P < 0.01) the total concentration of omega-6 fatty acids (n-6) when compared to chestnut diets, but no differences (P > 0.05) were observed between acorn and control diets. Inclusion of acorns reduced (P < 0.05) total saturated fatty acids (SFA) when compared to control and chestnut treatments; however total polyunsaturated fatty acids (PUFA) were increased (P < 0.05) when acorns were included in the diet. When evaluating PUFA:SFA ratio, no differences (P > 0.05) were found between control and chestnut diets. Including acorns in the diet, resulted in an increased (P < 0.05) PUFA:SFA ratio.ConclusionInclusion of acorns and chestnuts did not negatively impact carcass characteristics, carcass quality or bacon quality, nevertheless, including acorns altered overall fatty acid composition while minimal differences were observed between diets containing chestnuts and the control.

scholarly journals Dietary conjugated linoleic acid differentially alters fatty acid composition and increases conjugated linoleic acid content in porcine adipose tissue

2003 ◽  
Vol 90 (5) ◽  
pp. 915-928 ◽  
Author(s):  
Ewa Ostrowska ◽  
Reg F. Cross ◽  
Morley Muralitharan ◽  
Dale E. Bauman ◽  
Frank R. Dunshea
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Acid Composition ◽  
Subcutaneous Fat ◽  
Large White ◽  
Cla Isomers

Conjugated linoleic acids (CLA) have been shown to decrease body fat content in pigs. It is possible that feeding pigs diets rich in CLA may increase carcass lipid CLA to levels that could provide health benefits when included as a part of a healthy diet. Therefore, the aim of the present study was to determine whether dietary CLA supplementation has any effect on the fatty acid composition of subcutaneous and intramuscular adipose tissue in pigs. Thirty-five female cross bred (Large White×Landrace) pigs (initial weight 57·2kg and initial P2 back fat 11·5mm) were used in the present study. Pigs were housed individually and randomly allocated to one of six dietary treatments (0·00, 1·25, 2·50, 5·00, 7·50 and 10·00g CLA55 (55g CLA isomers/100g total fatty acids; Natural Lipids Ltd, Hovdebygda, Norway)/kg) and fed their respective diets for 8 weeks. Twelve CLA isomers in the diet and in pig tissue lipids were separated by Ag+-HPLC. CLA was incorporated at fivefold higher levels in subcutaneous fat as compared with intramuscular fat and in a dose-dependant manner. Overall, the transfer efficiency of CLA was maximized at 5·00g CLA55/kg. However, there was clear selectivity in the uptake or incorporation ofcis,trans-9,11 isomer over thetrans,cis-10,12 isomer. In general, CLA supplementation produced significant changes in skeletal muscle and adipose tissue fatty acid composition, indicating that dietary CLA had a potent affect on lipid transport and metabolismin vivo. Significant increases in myristic, palmitic and palmitoleic acids and a reduction in arachidonic acid were observed, suggesting an alteration in activity of Δ5-, Δ6- and Δ9-desaturases in pig adipose tissue. In conclusion, feeding pigs diets supplemented with CLA increases carcass lipid CLA, but also results in changes in the fatty acid profile in pig fat that could potentially outweigh the benefits of CLA.

scholarly journals SEED YIELD AND FATTY ACID COMPOSITION IN SESAME (Sesamum indicum L.) AS AFFECTED BY SILICON APPLICATION UNDER A SEMI-ARID CLIMATE

Agrociencia ◽  
2020 ◽  
Vol 54 (3) ◽  
pp. 367-376
Author(s):  
Abdul Manaf ◽  
Mehreen Shoukat ◽  
Ahmad Sher ◽  
Abdul Qayyum ◽  
Ahmad Nawaz
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Palmitic Acid ◽  
Seed Yield ◽  
Sesamum Indicum ◽  
Sesame Genotypes ◽  
Semi Arid

Sesame (Sesamum indicum L.) is a short duration, low input and highly drought tolerant conventional oilseed crop with high edible seed oil contents. This study was aimed to evaluate the response of four sesame genotypes to silicon (Si) application under a semi-arid climate. For this study, we hypothesized that Si application may improve seed yield, oil contents and fatty acid composition in sesame. The experimental design was a two factor-factorial randomized complete block, replicated four times, and the treatments were four sesame genotypes (TS-3, SG-120, SG-169 and SG-170) and three Si levels (0, 22 and 44 kg ha-1). The data was analyzed statistically with the ‘Statistics 8.1’ software. The genotype TS-3 had the highest (p£0.05) seed yield (479.1 kg ha-1), oil content (40.2%), oleic acid (41.8%), and the lowest content of palmitic acid (6.37%) and linoleic acid (38.5%). Silicon application significantly enhanced the seed yield, oil and unsaturated fatty acids (oleic and linoleic acid) contents and reduced the saturated fatty acid (palmitic and stearic acid) over control. The highest seed yield (487.8 kg ha-1), oil contents (38%), oleic acid (40.9%), linoleic acid (41.7%), as well as the lowest palmitic acid (6.49%) and stearic acid (3.66%) were recorded with the application of 44 kg Si ha-1. The seed yield of sesame genotypes followed the order TS-3>SG-120>SG-169>SG-170.

FEATURES OF THE OIL-BEARING-CROP (CHARTHAMUS) CULTIVATION UNDER THE CONTRASTIVE EDAPHIC-CLIMATIC CONDITIONS

2018 ◽  
pp. 31-37
Author(s):  
S. К. Temirbekova ◽  
Yu. V. Afanaseva ◽  
I. M. Kulikov ◽  
G. V. Metlina ◽  
S. A. Vasilchenko
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Acid Composition ◽  
Oil Content ◽  
Moscow Region ◽  
Average Weight ◽  
North Caucasus ◽  
Saratov Region ◽  
Rostov Region

The results of long-term studies of the biological, morphological and phenological features of the introduced new culture of safflower in the Central, Volga and North Caucasus regions are presented. Optimum parameters of depth of seeding (5-6 cm), seeding rates (300-350 thousand pieces/hectare or 12-14 kg), ensuring high productivity, oil content and quality of seeds are established. For the first time, the relationship between moisture availability of vegetation periods with accumulation of oil content and a change in the fatty acid composition was established. Oilseed (in untreated seeds) in the regions was from 14,5 to 31,2%, in excessively wet 2013 – 6,4% in the Moscow region and 8,6% in the Saratov region. Fatty acid composition revealed a high content of oleic acid in Krasa Stupinskaya variety – 13,6-16,8%, linoleic acid – 68,5-75,7%. The yield of oil in the Moscow region was 240 kg/ha. The yield of Krasa Stupinskaya in the Moscow Region was 0,6 t/ha, the Rostov Region 0,8 t/ha and Saratov Region 1,2 t/ha, with an average weight of 1000 seeds, respectively, by regions: 40,0 g, 47,3 g and 40,9 g. The growing season for growing seeds was 105 days in the Moscow Region, 94 days in the Rostov Region and 95 days in the Saratov Region. It has been established that excessive moistening during the flowering and seed filling period increases the harmfulness of enzyme-mycosis seed depletion (EMIS) – biological injury during maturation (enzymatic stage), followed by the seeding of the seeds with the phytopathogen Alternaria carthami Chowdhury. In the breeding programs for productivity and oil content, it is recommended to use the varieties Moldir (Kazakhstan) and Krasa Stupinskaya (FGBNU VSTISP), the fatty acid composition of which is characterized by an increased content of oleic and linoleic acid, which is of particular value for storage and use for food purposes.

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