scholarly journals Growth inhibitory action of saccharin and cyclamate on rats receiving a poor rice diet

1969 ◽  
Vol 23 (3) ◽  
pp. 505-509 ◽  
Author(s):  
M. I. Sabri ◽  
S. K. Sharma ◽  
C. R. Krishna Murti
Keyword(s):  
Inhibitory Action ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Succinate Dehydrogenase Activity ◽  
Deficient Diet ◽  
Balanced Diet ◽  
The Poor ◽  
Growth Inhibitory ◽  
Rice Diet

1. The effect of saccharin and cyclamate on growth of young rats fed on a poor rice diet or a balanced diet was investigated.2. Saccharin and cyclamate retarded the growth of rats on the multi-deficient diet but not of those on the well-balanced diet during an 8-week feeding period.3. The sweeteners did not produce any macroscopic or microscopic changes in the liver, kidneys, intestines, spleen or lungs of the animals receiving the poor rice diet other than the changes resulting from the nutritional deficiency of the diet.4. The sweeteners did not inhibit the liver xanthine oxidase activity of rats receiving the poor rice diet to an extent greater than the inhibition brought about by the deficiency of protein in the diet.5. When given by intubation to healthy rats, the sweeteners inhibited the induction of liver tryptophan oxygenase; given in vitro, they inhibited the succinate dehydrogenase activity of rat liver mitochondria.

scholarly journals Influence of starvation and clofibrate administration on oxidative phosphorylation by rat liver mitochondria

1980 ◽  
Vol 190 (1) ◽  
pp. 191-198 ◽  
Author(s):  
B K A Rasheed ◽  
S Chhabra ◽  
C K R Kurup
Keyword(s):  
Cytochrome C ◽  
Binding Sites ◽  
Liver Mitochondria ◽  
Potassium Ferricyanide ◽  
Rat Liver Mitochondria ◽  
Albino Rats ◽  
Succinate Dehydrogenase Activity ◽  
Whole Cells ◽  
High Affinity

Whole cells, homogenates and mitochondrial obtained from the livers of albino rats which were starved for 6 days or more showed a 50% decrease in oxidative activity. The decrease could be corrected by the addition of cytochrome c in vitro. The phosphorylative activity of mitochondria remained unaffected. The decrease in oxidative rate was not observed when starving animals were given the anti-hypercholesterolaemic drug clofibrate. The total cellular concentration of cytochrome c was not affected by starvation. However, the concentration of the pigment in hepatic mitochondria isolated from starving animals was less than half that in normal mitochondria. Clofibrate-treated animals did not show a decreased concentration of cytochrome c in hepatic mitochondria. Mitochondria isolated from starving animals, though deficient in cytochrome c, did not show any decrease in succinate dehydrogenase activity or in the rate of substrate-dependent reduction of potassium ferricyanide or attendant phosphorylation. In coupled mitochondria, ferricyanide may not accept electrons from the cytochrome c in the respiratory chain. Starvation decreases the concentration of high-affinity binding sites for cytochrome c on the mitochondrial membrane. The dissociation constant increases in magnitude.

Magnetic nanostructured lipid carrier for dual triggered curcumin delivery: Preparation, characterization and toxicity evaluation on isolated rat liver mitochondria

2021 ◽  
pp. 088532822110346
Author(s):  
Mohammad Yoozbashi ◽  
Hamid Rashidzadeh ◽  
Mehraneh Kermanian ◽  
Somayeh Sadighian ◽  
Mir-Jamal Hosseini ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Nanostructured Lipid Carrier ◽  
Mitochondrial Toxicity ◽  
X Ray Diffraction ◽  
Temperature Dependent ◽  
Reducing Ability ◽  
Transmission Electron

In this research, magnetic nanostructured lipid carriers (Mag-NLCs) were synthesized for curcumin (CUR) delivery. NLCs are drug-delivery systems prepared by mixing solid and liquid (oil) lipids. For preparation of NLCs, cetylpalmitate was selected as solid lipid and fish oil as liquid lipid. CUR-Mag-NLCs were prepared using high-pressure homogenization technique and were characterized by methods including X-ray diffraction (XRD), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), and dynamic light scattering (DLS). The CUR-Mag-NLCs were developed as a particle with a size of 140 ± 3.6 nm, a polydispersity index of 0.196, and a zeta potential of −22.6 mV. VSM analysis showed that the CUR-Mag-NLCs have excellent magnetic properties. Release rate of the drug was higher at 42 °C than 37 °C, indicating that release of the synthesized nanoparticles is temperature-dependent. Evaluation of mitochondrial toxicity was done using the isolated rats liver mitochondria including glutathione (GSH), malondialdehyde (MDA), and the ferric- reducing ability of plasma (FRAP) assays to study biosafety of the CUR-Mag-NLCs. Results of In vitro study on the isolated mitochondria revealed that both CUR-Mag-NLCs and curcumin have no specific mitochondrial toxicity.

scholarly journals Fluorocitrate inhibition of aconitate hydratase and the tricarboxylate carrier of rat liver mitochondria

1973 ◽  
Vol 134 (1) ◽  
pp. 217-224 ◽  
Author(s):  
M. D. Brand ◽  
Susan M. Evans ◽  
J. Mendes-Mourão ◽  
J. B. Chappell
Keyword(s):  
Rat Liver ◽  
Inhibitory Action ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Exchange Reactions ◽  
Aconitate Hydratase ◽  
Isolated Rat Liver ◽  
Tricarboxylate Carrier ◽  
Hydratase Activity ◽  
Isolated Rat

1. The effect of biologically synthesized and purified fluorocitrate on the metabolism of tricarboxylate anions by isolated rat liver mitochondria was investigated, in relation to the claim by Eanes et al. (1972) that this fluoro compound inhibits the tricarboxylate carrier at concentrations at which it has little effect on the aconitate hydratase activity. 2. That the inhibitory action of fluorocitrate is at the level of the aconitate hydratase and not at the level of the tricarboxylate carrier is indicated by the following findings. Although the oxidation of citrate and cis-aconitate, but not that of isocitrate, was inhibited by fluorocitrate, the exchange of internal citrate for external citrate or l-malate was not. Had the tricarboxylate carrier been affected, these latter exchange reactions would have been inhibited. 3. By using aconitate hydratase solubilized from mitochondria it was found that with citrate as substrate the inhibition by fluorocitrate was partially competitive (Ki=3.4×10−8m), whereas with cis-aconitate as substrate the inhibition was partially non-competitive (Ki=3.0×10−8m).

IN VITRO LIPID PEROXIDATION INHIBITORY AND ANTI-ARTHRITIC ACTIVITIES OF SOME INDIAN MEDICINAL PLANTS

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (06) ◽  
pp. 31-35
Author(s):  
A. A Rege ◽  
◽  
P. R. Juvekar ◽  
A. R. Juvekar
Keyword(s):  
Lipid Peroxidation ◽  
Liver Mitochondria ◽  
Tinospora Cordifolia ◽  
Ocimum Sanctum ◽  
Rat Liver Mitochondria ◽  
Total Phenolic ◽  
Lipid Peroxidation Inhibitory Activity ◽  
Gallic Acid Equivalent ◽  
Indian Medicinal Plants

Anti-lipid peroxidation effect of aqueous extracts of Ocimum sanctum, Tinospora cordifolia and Withania somnifera was evaluated against Fe2+-ascorbic acid-induced lipid peroxidation using rat liver mitochondria as model system, whereas, anti-arthritic activity was evaluated by proteinase inhibitory assay. O. sanctum showed potent anti-lipid peroxidation and anti-arthritic activities. T. cordifolia exhibited moderate anti-lipid peroxidation activity, but considerable anti-arthritic activity, whereas, W. somnifera revealed least lipid peroxidation inhibitory activity and considerable anti-arthritic activity. Besides, Folin-Ciocalteu reagent in terms of gallic acid equivalent achieved the total phenolic content and the trend was found to be O. sanctum > T. cordifolia > W. somnifera.

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