scholarly journals Involvement of the Helix-Loop-Helix Protein Id-1 in the Glucocorticoid Regulation of Tight Junctions in Mammary Epithelial Cells

2000 ◽  
Vol 275 (37) ◽  
pp. 28649-28658 ◽  
Author(s):  
Paul L. Woo ◽  
Andrea Cercek ◽  
Pierre-Yves Desprez ◽  
Gary L. Firestone
Keyword(s):  
Epithelial Cells ◽  
Tight Junctions ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Helix Loop Helix

Phytoestrogens Weaken the Blood–Milk Barrier in Lactating Mammary Epithelial Cells by Affecting Tight Junctions and Cell Viability

2017 ◽  
Vol 65 (50) ◽  
pp. 11118-11124 ◽  
Author(s):  
Yusaku Tsugami ◽  
Kota Matsunaga ◽  
Takahiro Suzuki ◽  
Takanori Nishimura ◽  
Ken Kobayashi
Keyword(s):  
Epithelial Cells ◽  
Cell Viability ◽  
Tight Junctions ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial

scholarly journals Mammary Epithelial Cells Are Not Able to Undergo Pregnancy-Dependent Differentiation in the Absence of the Helix-Loop-Helix Inhibitor Id2

2002 ◽  
Vol 16 (12) ◽  
pp. 2892-2901 ◽  
Author(s):  
Keiko Miyoshi ◽  
Barbara Meyer ◽  
Peter Gruss ◽  
Yongzhi Cui ◽  
Jean-Pierre Renou ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Early Stage ◽  
Prolactin Receptor ◽  
Signal Transduction Pathway ◽  
Mammary Epithelium ◽  
Mammary Epithelial ◽  
Differentiation Markers ◽  
Helix Loop Helix ◽  
Alveolar Development

Abstract Mammary alveolar development during pregnancy is triggered by hormone signals. The prolactin receptor/Jak2/signal transducer and activator of transcription (Stat) 5 signal transduction pathway is the principal mediator of these cues and alveolar development is abrogated in its absence. The loss of the basic helix-loop-helix protein inhibitor of differentiation (Id)2 results in a similar defect. To investigate the role of Id2 in mammary epithelium, we performed structural and molecular analyses. Id2-null mammary epithelial cells were unable to form alveoli; the epithelial architecture was disorganized and dissimilar from early stages of alveologenesis in wild-type glands. The epithelial cells retained the ductal marker Na-K-Cl cotransporter (NKCC)1. Nuclear localization of Stat5a and down-regulation of NKCC1 was observed in some areas, indicating a limited response to pregnancy signals. The differentiation status of Id2-null tissue at term was further characterized with cDNA microarrays enriched in mammary specific sequences (mammochip). Some of the early differentiation markers for mammary epithelium were expressed in the Id2-null tissue, whereas genes that are expressed at later stages of pregnancy were not induced. From these results, we conclude that, in the absence of Id2, mammary epithelial development is arrested at an early stage of pregnancy.

scholarly journals Glucocorticoid-induced formation of tight junctions in mouse mammary epithelial cells in vitro.

1992 ◽  
Vol 89 (19) ◽  
pp. 9069-9073 ◽  
Author(s):  
K. S. Zettl ◽  
M. D. Sjaastad ◽  
P. M. Riskin ◽  
G. Parry ◽  
T. E. Machen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tight Junctions ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial
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