scholarly journals Characterization of the Effects of Mutations in the Putative Branchpoint Sequence of Intron 4 on the Splicing within the Human Lecithin:cholesterol Acyltransferase Gene

2000 ◽  
Vol 275 (24) ◽  
pp. 18079-18084 ◽  
Author(s):  
Min Li ◽  
P. Haydn Pritchard
Keyword(s):  
Lecithin:Cholesterol Acyltransferase ◽  
Branchpoint Sequence ◽  
Intron 4

scholarly journals Characterization of C-terminal histidine-tagged human recombinant lecithin:cholesterol acyltransferase

1999 ◽  
Vol 40 (8) ◽  
pp. 1512-1519 ◽  
Author(s):  
Jeffrey W. Chisholm ◽  
Abraham K. Gebre ◽  
John S. Parks
Keyword(s):  
Lecithin:Cholesterol Acyltransferase

Identification and characterization of a novel tobacco mosaic virus resistance N gene homologue in Nicotiana tabacum plants

2004 ◽  
Vol 31 (2) ◽  
pp. 149 ◽  
Author(s):  
Claudia Stange ◽  
José Tomás Matus ◽  
Alvaro Elorza ◽  
Patricio Arce-Johnson
Keyword(s):  
Nicotiana Tabacum ◽  
N Gene ◽  
Post Inoculation ◽  
Coding Region ◽  
Hypersensitive Resistance ◽  
Base Pairs ◽  
Resistance Response ◽  
Intron 4 ◽  
Gene Homologue

Nicotiana tabacum cv. Xanthi nn plants are susceptible to infection by most tobamoviruses (TMV). However, such plants display a partial hypersensitive resistance response (HR-like response) to TMV-Cg. The genetic mechanism of the HR-like response has yet not been determined, but it may involve a gene with a function similar to that of a resistance gene, responsible for HR in resistant plants. We have cloned a gene homologous to the resistance N gene, named NH, from Nicotiana Xanthi nn plants. The coding region of NH is 5.028 base pairs (bp) long and has 82.6% nucleotide identity with the N gene. In contrast to the N gene, the NH gene lacks intron 4 and does not have sites for alternative splicing of intron 3. Analysis of its sequence revealed that NH belongs to the TIR / NSB / LRR gene class. We were able to detect stable levels of NH-transcript in Nicotiana Xanthi nn plants from 0 to 18 h post-inoculation (hpi) with TMV-Cg. Transcript levels increased slightly at 24 hpi and dropped below basal values at 48 hpi. The NH transcript was also detected in a range of resistant Nicotiana plants (N. tabacum Xanthi NN, N. glutinosa, N. glauca and N. rustica) suggesting that NH is a homologue of the N gene, rather than an allele. We have cloned and characterised the NH gene (GenBank acc. no. bankit598573 AY535010) from nn susceptible plants and postulate that this gene might be involved in the HR-like response seen in these plants.

Characterization of subspecies of lipoprotein containing apolipoprotein A-I in heterozygotes for familial lecithin:cholesterol acyltransferase deficiency

1995 ◽  
Vol 114 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Takao Ohta ◽  
Rie Nakamura ◽  
Jiri J. Frohlich ◽  
P.Haydn Pritchard ◽  
Ichiro Matsuda
Keyword(s):  
Lecithin:Cholesterol Acyltransferase ◽  
Apolipoprotein A

Characterization of lecithin:Cholesterol acyltransferase from human plasma

1983 ◽  
Vol 222 (2) ◽  
pp. 553-560 ◽  
Author(s):  
Kui-Song Chong ◽  
Shinichi Hara ◽  
Richard E. Thompson ◽  
Andras G. Lacko
Keyword(s):  
Human Plasma ◽  
Lecithin:Cholesterol Acyltransferase

scholarly journals Expression and characterization of recombinant human lecithin:cholesterol acyltransferase

1993 ◽  
Vol 34 (7) ◽  
pp. 1245-1251
Author(s):  
JS Hill ◽  
K O ◽  
X Wang ◽  
S Paranjape ◽  
D Dimitrijevich ◽  
...  
Keyword(s):  
Lecithin:Cholesterol Acyltransferase

Characterization of waxy proteins and waxy genes of Triticum timopheevii and T. zhukovskyi and implications for evolution of wheat

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 582-588 ◽  
Author(s):  
Liuling Yan ◽  
Mrinal Bhave
Keyword(s):  
Triticum Timopheevii ◽  
Wheat Evolution ◽  
Waxy Protein ◽  
Waxy Proteins ◽  
B1 Gene ◽  
Genes Encoding ◽  
Gbss I ◽  
Intron 4 ◽  
Specific Sequences

The granule-bound starch (GBSS I, waxy protein) in Triticum timopheevii (AtAtGG) and T. zhukovskyi (AtAtAzAzGG) and a diagnostic section of the genes encoding GBSS-I from the Wx-TtA and Wx-G loci of T. timopheevii and the Wx-TtA, Wx-G, and Wx-TzA loci of T. zhukovskyi were investigated in this study. The waxy proteins in these two polyploid wheats could not be separated into distinct bands, in contrast to those in the T. turgidum (AABB) – T. aestivum (AABBDD) lineage. Alignment of sequences of the section covering exon4–intron4–exon5 of the various waxy genes led to the identification of gene-specific sequences in intron 4. The sequences specific to the Wx-TtA and Wx-G genes of T. timopheevii were different from those of the Wx-A1 gene and Wx-B1 genes of T. turgidum and T. aestivum. A surprising observation was that the Wx-TzA of T. zhukovskyi did not match with the Wx-TmA of T. monococcum, a putative donor of the Az genome, but matched unexpectedly and perfectly with the Wx-B1 gene on chromosome 4A, which is proposed to have translocated from the chromosome 7B of T. aestivum. The possible genetic mechanism explaining these observations is discussed.Key words: waxy proteins, waxy genes, T. timopheevii, T. zhukovskyi, wheat evolution.

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