scholarly journals The High Osmotic Response and Cell Wall Integrity Pathways Cooperate to Regulate Transcriptional Responses to Zymolyase-induced Cell Wall Stress inSaccharomyces cerevisiae

2009 ◽  
Vol 284 (16) ◽  
pp. 10901-10911 ◽  
Author(s):  
Raúl García ◽  
Jose M. Rodríguez-Peña ◽  
Clara Bermejo ◽  
César Nombela ◽  
Javier Arroyo
Keyword(s):  
Cell Wall ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Transcriptional Responses ◽  
Cell Wall Stress ◽  
Osmotic Response

scholarly journals A novel connection between the Cell Wall Integrity and the PKA pathways regulates cell wall stress response in yeast

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Raúl García ◽  
Enrique Bravo ◽  
Sonia Diez-Muñiz ◽  
Cesar Nombela ◽  
Jose M. Rodríguez-Peña ◽  
...  
Keyword(s):  
Cell Wall ◽  
Stress Response ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Cell Wall Stress

scholarly journals KRE5 Suppression Induces Cell Wall Stress and Alternative ER Stress Response Required for Maintaining Cell Wall Integrity in Candida glabrata

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0161371 ◽  
Author(s):  
Yutaka Tanaka ◽  
Masato Sasaki ◽  
Fumie Ito ◽  
Toshio Aoyama ◽  
Michiyo Sato-Okamoto ◽  
...  
Keyword(s):  
Cell Wall ◽  
Stress Response ◽  
Er Stress ◽  
Candida Glabrata ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Er Stress Response ◽  
Cell Wall Stress

scholarly journals The Sequential Activation of the Yeast HOG and SLT2 Pathways Is Required for Cell Survival to Cell Wall Stress

2008 ◽  
Vol 19 (3) ◽  
pp. 1113-1124 ◽  
Author(s):  
Clara Bermejo ◽  
Estefanía Rodríguez ◽  
Raúl García ◽  
Jose M. Rodríguez-Peña ◽  
María L. Rodríguez de la Concepción ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Survival ◽  
Wall Stress ◽  
Hog Pathway ◽  
Transcriptional Responses ◽  
Cell Wall Stress ◽  
Cell Wall Damage ◽  
Mapk Pathways ◽  
Essential Components ◽  
Sequential Activation

Yeast mitogen-activated protein kinase (MAPK) signaling pathways transduce external stimuli into cellular responses very precisely. The MAPKs Slt2/Mpk1 and Hog1 regulate transcriptional responses of adaptation to cell wall and osmotic stresses, respectively. Unexpectedly, we observe that the activation of a cell wall integrity (CWI) response to the cell wall damage caused by zymolyase (β-1,3 glucanase) requires both the HOG and SLT2 pathways. Zymolyase activates both MAPKs and Slt2 activation depends on the Sho1 branch of the HOG pathway under these conditions. Moreover, adaptation to zymolyase requires essential components of the CWI pathway, namely the redundant MAPKKs Mkk1/Mkk2, the MAPKKK Bck1, and Pkc1, but it does not require upstream elements, including the sensors and the guanine nucleotide exchange factors of this pathway. In addition, the transcriptional activation of genes involved in adaptation to cell wall stress, like CRH1, depends on the transcriptional factor Rlm1 regulated by Slt2, but not on the transcription factors regulated by Hog1. Consistent with these findings, both MAPK pathways are essential for cell survival under these circumstances because mutant strains deficient in different components of both pathways are hypersensitive to zymolyase. Thus, a sequential activation of two MAPK pathways is required for cellular adaptation to cell wall damage.

scholarly journals Transcriptional Profiling Reveals that Daptomycin Induces the Staphylococcus aureus Cell Wall Stress Stimulon and Genes Responsive to Membrane Depolarization

2007 ◽  
Vol 52 (3) ◽  
pp. 980-990 ◽  
Author(s):  
Arunachalam Muthaiyan ◽  
Jared A. Silverman ◽  
Radheshyam K. Jayaswal ◽  
Brian J. Wilkinson
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Mode Of Action ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Transcriptional Profiling ◽  
Wall Stress ◽  
Membrane Depolarization ◽  
Transcriptional Responses ◽  
Cell Wall Stress

ABSTRACT Daptomycin is a lipopeptide antibiotic that has recently been approved for treatment of gram-positive bacterial infections. The mode of action of daptomycin is not yet entirely clear. To further understand the mechanism transcriptomic analysis of changes in gene expression in daptomycin-treated Staphylococcus aureus was carried out. The expression profile indicated that cell wall stress stimulon member genes (B. J. Wilkinson, A. Muthaiyan, and R. K. Jayaswal, Curr. Med. Chem. Anti-Infect. Agents 4:259-276, 2005) were significantly induced by daptomycin and by the cell wall-active antibiotics vancomycin and oxacillin. Comparison of the daptomycin response of a two-component cell wall stress stimulon regulator VraSR mutant, S. aureus KVR, to its parent N315 showed diminished expression of the cell wall stress stimulon in the mutant. Daptomycin has been proposed to cause membrane depolarization, and the transcriptional responses to carbonyl cyanide m-chlorophenylhydrazone (CCCP) and nisin were determined. Transcriptional profiles of the responses to these antimicrobial agents showed significantly different patterns compared to those of the cell wall-active antibiotics, including little or no induction of the cell wall stress stimulon. However, there were a significant number of genes induced by both CCCP and daptomycin that were not induced by oxacillin or vancomycin, so the daptomycin transcriptome probably reflected a membrane depolarizing activity of this antimicrobial also. The results indicate that inhibition of peptidoglycan biosynthesis, either directly or indirectly, and membrane depolarization are parts of the mode of action of daptomycin.

scholarly journals Aspergillus fumigatus Hsp90 interacts with the main components of the cell wall integrity pathway and cooperates in heat shock and cell wall stress adaptation

2020 ◽  
Author(s):  
Marina Campos Rocha ◽  
Karine Minari ◽  
João Henrique Tadini Marilhano Fabri ◽  
Joshua D. Kerkaert ◽  
Lisandra Marques Gava ◽  
...  
Keyword(s):  
Cell Wall ◽  
Heat Shock ◽  
Aspergillus Fumigatus ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Stress Adaptation ◽  
Cell Wall Stress ◽  
Cell Wall Integrity Pathway ◽  
Main Components

scholarly journals Author Correction: Signalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Raúl García ◽  
Verónica Pulido ◽  
Sara Orellana-Muñoz ◽  
César Nombela ◽  
Carlos R. Vázquez de Aldana ◽  
...  
Keyword(s):  
Cell Wall ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Cell Wall Stress ◽  
Cell Wall Integrity Pathway

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