scholarly journals Characterization of Enantiomeric Bile Acid-induced Apoptosis in Colon Cancer Cell Lines

2008 ◽  
Vol 284 (5) ◽  
pp. 3354-3364 ◽  
Author(s):  
Bryson W. Katona ◽  
Shrikant Anant ◽  
Douglas F. Covey ◽  
William F. Stenson
Keyword(s):  
Colon Cancer ◽  
Bile Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Colon Cancer Cell Lines ◽  
Induced Apoptosis

Bile acid mediated apoptosis in human colon cancer cell lines is not mediated via the CD95/Fas/Apo-1 pathway

2000 ◽  
Vol 118 (4) ◽  
pp. A1140
Author(s):  
Frank-Peter Wachs ◽  
Gerhard Rogler ◽  
Rene C. Krieg ◽  
Frank Kullmann ◽  
Helmut Messmann ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Bile Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Colon Cancer Cell Lines

scholarly journals Capsaicin induces apoptosis by generating reactive oxygen species and disrupting mitochondrial transmembrane potential in human colon cancer cell lines

2009 ◽  
Vol 14 (3) ◽  
Author(s):  
Kyung Yang ◽  
Jong Pyo ◽  
Gyu-Yeol Kim ◽  
Rina Yu ◽  
In Han ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Caspase 3 ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Enzyme Treatment ◽  
Dependent Manner ◽  
Colon Cancer Cell Lines ◽  
Induced Apoptosis

AbstractAlthough genetic factors are a well-known cause of colorectal cancer, environmental factors contribute more to its development. Despite advances in the fields of surgery, radiotherapy and chemotherapy, the cure rates for colon cancer have not substantially improved over the past few decades. Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide), the principal pungent ingredient of hot chili pepper, has exhibited an anti-tumor effect in many cell types. However, the mechanisms responsible for the anti-tumor effect of capsaicin are not yet completely understood. In this study, we investigated whether capsaicin induces apoptosis in colon cancer cell lines. Capsaicin decreased cell viability in a dose-dependent manner in Colo320DM and LoVo cells. In addition, capsaicin produced cell morphology changes and DNA fragmentation, decreased the DNA contents, and induced phosphatidylserine translocation, which is a hallmark of apoptotic cell death. We showed that capsaicin-induced apoptosis is associated with an increase in ROS generation and a disruption of the mitochondrial transmenbrane potential. A possible mechanism of capsaicin-induced apoptosis is the activation of caspase 3, a major apoptosis-executing enzyme. Treatment with capsaicin induced a dramatic increase in caspase 3 activity, as assessed by the cleavage of Ac-DEVD-AMC, a fluorogenic substrate. In conclusion, our results clearly showed that capsaicin induced apoptosis in colon cancer cells. Although the actual mechanisms of capsaicin-induced apoptosis remain uncertain, it may be a beneficial agent for colon cancer treatment and chemoprevention.

Bile salt induced apoptosis in colon cancer cell lines: Mechanisms and differences to primary colon epithelial cells

2000 ◽  
Vol 118 (4) ◽  
pp. A553
Author(s):  
Klaus Schlottmann ◽  
Frank-Peter Wachs ◽  
Rene C. Krieg ◽  
Juergen Schoelmerich ◽  
Gerhard Rogler
Keyword(s):  
Colon Cancer ◽  
Epithelial Cells ◽  
Bile Salt ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Colon Cancer Cell Lines ◽  
Primary Colon ◽  
Induced Apoptosis

Production and characterization of stable 177Lu–siRNA complex and kinetic studies in colon cancer cell lines

2011 ◽  
Vol 44 (13) ◽  
pp. S72
Author(s):  
Mojtaba Fathi ◽  
Kamal Yavari ◽  
Mohammad Taghikhani ◽  
Mohammad Ghannadi Maragheh
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Kinetic Studies ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Colon Cancer Cell Lines

Arsenic trioxide-induced apoptosis through oxidative stress in cells of colon cancer cell lines

Life Sciences ◽  
2002 ◽  
Vol 70 (19) ◽  
pp. 2253-2269 ◽  
Author(s):  
Yoshihito Nakagawa ◽  
Yukihiro Akao ◽  
Hiroshi Morikawa ◽  
Ichiro Hirata ◽  
Kenichi Katsu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Colon Cancer ◽  
Arsenic Trioxide ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Colon Cancer Cell Lines ◽  
Induced Apoptosis ◽  
In Cells

Isolation and characterization of two novel colon cancer cell lines from Chinese patients

2007 ◽  
Vol 43 (3-4) ◽  
pp. 105-108 ◽  
Author(s):  
Dan-Ning Hu ◽  
Shiaw-Min Hwang ◽  
Xi-Zhang Lin ◽  
Pei-Yuh Yang ◽  
Chi-Hong Tsai ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Chinese Patients ◽  
Isolation And Characterization ◽  
Colon Cancer Cell Lines

Isolation of Cardamonin and Pinostrobin Chalcone from the Rhizomes of Boesenbergia rotunda (L.) Mansf. and their Cytotoxic Effects on H-29 and MDA-MB-231 Cancer Cell Lines

2019 ◽  
Vol 9 (4) ◽  
pp. 341-348 ◽  
Author(s):  
Ibrahim Awad Mohammed ◽  
Muhammad Nadeem Akhtar ◽  
Foo Jhi Biau ◽  
Yin Sim Tor ◽  
Seema Zareen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Chemical Constituents ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Colon Cancer Cell Lines ◽  
Ht 29

<P>Background: Breast cancer and human colon cancer are the most common types of cancer in females and males, respectively. Breast cancer is the most common type of cancer after lung and colon cancers. Natural products are an important source for drug discovery. Boesenbergia rotunda (L.) Mansf. is commonly known as finger root, belonging to the Zingiberaceae family. </P><P> Objective: The aim of this study to isolate some natural compounds from the rhizomes of B. rotunda (L.) Mansf., and to investigate their cytotoxicity against the human triple-negative breast cancer cell (MDA-MB-231) and HT-29 colon cancer cell lines. </P><P> Methods: The dried rhizomes of B. rotunda were extracted with methanol. The methanolic extract was further used for solvent-solvent extraction. Bioassay-guided extraction and isolation of the rhizomes of the B. rotunda exhibited cytotoxic properties of hexane and dichloromethane fractions. </P><P> Results: Six major chemical constituents, pinostrobin (1), pinostrobin chalcone (2), cardamonin (3), 4,5-dihydrokawain (4), pinocembrin (5), and alpinetin (6) were isolated from the rhizomes of the B. rotunda. All the chemical constituents were screened against the human triple-negative breast cancer cell (MDA-MB-231) and HT-29 colon cancer cell lines. The compound cardamonin (3) (IC50 = 5.62&#177;0.61 and 4.44&#177;0.66 &#181;g/mL) and pinostrobin chalcone (2), (IC50 = 20.42&#177;2.23 and 22.51&#177;0.42 μg/mL) were found to be potent natural cytotoxic compounds against MDA-MB-231 and HT-29 colon cancer cell lines, respectively. </P><P> Conclusion: Cardamonin (3) and pinostrobin chalcone (2) were found to be the most potential natural compounds against breast cancer cell line MDA-MB-231 and colon cancer HT-29 cell line.</P>

scholarly journals Reactivity of Monoclonal Antibodies Directed against Lung Cancer Antigens with Human Lung, Breast and Colon Cancer Cell Lines

1993 ◽  
Vol 11 (5-6) ◽  
pp. 225-237
Author(s):  
Udo Schumacher ◽  
Dhia Mukthar ◽  
Thomas Schenker
Keyword(s):  
Breast Cancer ◽  
Lung Cancer ◽  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Breast Cancer Cell Lines ◽  
Cancer Antigens ◽  
Colon Cancer Cell Lines

A panel of monoclonal antibodies (n=72 including controls) directed against lung cancer antigens was screened immunohistochemically against a panel of seven human lung cancer cell lines (including small cell carcinoma, squamous cell carcinoma, adenocarcinoma and mesothelioma), six human breast cancer cell lines and one human colon cancer cell line, The majority of the antibodies (n=42) reacted also with antigens present on breast and colon cancer cell lines, This cross reactivity especially between lung and breast cancer cell lines is not altogether unexpected since antigens common to breast and lung tissue including their neoplasms such as MUC1 antigen have been described, Our results indicate that epitopes shared by lung and breast cancers are probably more common than previously thought. The relevance for prognosis and therapy of these shared antigens, especially as disease markers in breast cancer, has to be investigated.

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