scholarly journals Vibrio vulnificus rpoSExpression Is Repressed by Direct Binding of cAMP-cAMP Receptor Protein Complex to Its Two Promoter Regions

2008 ◽  
Vol 283 (45) ◽  
pp. 30438-30450 ◽  
Author(s):  
Hyun-Jung Lee ◽  
Soon-Jung Park ◽  
Sang Ho Choi ◽  
Kyu-Ho Lee
Keyword(s):  
Protein Complex ◽  
Vibrio Vulnificus ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Promoter Regions ◽  
Direct Binding ◽  
Camp Receptor

scholarly journals Repression of VvpM Protease Expression by Quorum Sensing and the cAMP-cAMP Receptor Protein Complex inVibrio vulnificus

2018 ◽  
Vol 200 (7) ◽  
Author(s):  
Jeong-A Kim ◽  
Mi-Ae Lee ◽  
You-Chul Jung ◽  
Bo-Ram Jang ◽  
Kyu-Ho Lee
Keyword(s):  
Transcription Factors ◽  
Stationary Phase ◽  
Cell Density ◽  
Transcription Initiation ◽  
Vibrio Vulnificus ◽  
The Other ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Content Type ◽  
Camp Receptor

ABSTRACTSepticemia-causingVibrio vulnificusproduces at least three exoproteases, VvpE, VvpS, and VvpM, all of which participate in interactions with human cells. Expression of VvpE and VvpS is induced in the stationary phase by multiple transcription factors, including sigma factor S, SmcR, and the cAMP-cAMP receptor protein (cAMP-CRP) complex. Distinct roles of VvpM, such as induction of apoptosis, lead us to hypothesize VvpM expression is different from that of the other exoproteases. Its transcription, which was found to be independent of sigma S, is induced at the early exponential phase and then becomes negligible upon entry into the stationary phase. SmcR and CRP were studied regarding the control ofvvpMexpression. Transcription ofvvpMwas repressed by SmcR and cAMP-CRP complex individually, which specifically bound to the regions −2 to +20 and +6 to +27, respectively, relative to thevvpMtranscription initiation site. Derepression ofvvpMgene expression was 10- to 40-fold greater in ansmcR crpdouble mutant than in single-gene mutants. Therefore, these results show that the expression ofV. vulnificusexoproteases is differentially regulated, and in this way, distinct proteases can engage in specific interactions with a host.IMPORTANCEAn opportunistic human pathogen,Vibrio vulnificusproduces multiple extracellular proteases that are involved in diverse interactions with a host. The total exoproteolytic activity is detected mainly in the supernatants of the high-cell-density cultures. However, some proteolytic activity derived from a metalloprotease, VvpM, was present in the supernatants of the low-cell-density cultures sampled at the early growth period. In this study, we present the regulatory mechanism for VvpM expression via repression by at least two transcription factors. This type of transcriptional regulation is the exact opposite of those for expression of the otherV. vulnificusexoproteases. Differential regulation of each exoprotease's production then facilitates the pathogen's participation in the distinct interactions with a host.

scholarly journals The cyclic AMP (cAMP)-cAMP receptor protein complex functions both as an activator and as a corepressor at the tsx-p2 promoter of Escherichia coli K-12.

1991 ◽  
Vol 173 (17) ◽  
pp. 5419-5430 ◽  
Author(s):  
P Gerlach ◽  
L Søgaard-Andersen ◽  
H Pedersen ◽  
J Martinussen ◽  
P Valentin-Hansen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cyclic Amp ◽  
Protein Complex ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Complex Functions ◽  
P2 Promoter ◽  
Camp Receptor ◽  
K 12

scholarly journals Evidence that Expression of the Vibrio vulnificus Hemolysin Gene Is Dependent on Cyclic AMP and Cyclic AMP Receptor Protein

1999 ◽  
Vol 181 (24) ◽  
pp. 7639-7642 ◽  
Author(s):  
Young Bae Bang ◽  
Shee Eun Lee ◽  
Joon Haeng Rhee ◽  
Sang Ho Choi
Keyword(s):  
Escherichia Coli ◽  
Cyclic Amp ◽  
Promoter Activity ◽  
Vibrio Vulnificus ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Transcriptional Fusion ◽  
Cyclic Amp Receptor Protein ◽  
Hemolysin Gene ◽  
Camp Receptor

ABSTRACT Glucose repressed hemolysin production in Vibrio vulnificus. Promoter activity of the hemolysin gene,vvh, assessed with a vvh-luxCDABEtranscriptional fusion, required cyclic AMP (cAMP) and cAMP receptor protein (CRP) in Escherichia coli. Hemolysin production inV. vulnificus increased after the addition of cAMP and was undetectable in a putative crp mutant, suggesting thatvvh is also regulated by cAMP-CRP in V. vulnificus.

scholarly journals The cAMP receptor protein of Trypanosoma cruzi.

1983 ◽  
Vol 258 (11) ◽  
pp. 6979-6983 ◽  
Author(s):  
R Rangel-Aldao ◽  
G Tovar ◽  
M Ledezma de Ruiz
Keyword(s):  
Trypanosoma Cruzi ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Camp Receptor
Sign in / Sign up

Export Citation Format

Share Document