scholarly journals Ligand Dynamics and Early Signaling Events in the Heme Domain of the Sensor Protein Dos fromEscherichia coli

2007 ◽  
Vol 283 (4) ◽  
pp. 2344-2352 ◽  
Author(s):  
Taku Yamashita ◽  
Latifa Bouzhir-Sima ◽  
Jean-Christophe Lambry ◽  
Ursula Liebl ◽  
Marten H. Vos
Keyword(s):  
Fromescherichia Coli ◽  
Sensor Protein ◽  
Signaling Events ◽  
Heme Domain

Ligand Binding Dynamics to the Heme Domain of the Oxygen Sensor Dos fromEscherichia coli

Biochemistry ◽  
2003 ◽  
Vol 42 (21) ◽  
pp. 6527-6535 ◽  
Author(s):  
Ursula Liebl ◽  
Latifa Bouzhir-Sima ◽  
Laurent Kiger ◽  
Michael C. Marden ◽  
Jean-Christophe Lambry ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Oxygen Sensor ◽  
Fromescherichia Coli ◽  
Heme Domain

scholarly journals Heme Ligand Binding Properties and Intradimer Interactions in the Full-length Sensor Protein Dos from Escherichia coli and Its Isolated Heme Domain

2009 ◽  
Vol 284 (52) ◽  
pp. 36146-36159 ◽  
Author(s):  
Christophe Lechauve ◽  
Latifa Bouzhir-Sima ◽  
Taku Yamashita ◽  
Michael C. Marden ◽  
Marten H. Vos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ligand Binding ◽  
Full Length ◽  
Binding Properties ◽  
Sensor Protein ◽  
Heme Domain

Unusual Dynamics of Ligand Binding to the Heme Domain of the Bacterial CO Sensor Protein RcoM-2

2016 ◽  
Vol 120 (41) ◽  
pp. 10686-10694 ◽  
Author(s):  
Latifa Bouzhir-Sima ◽  
Roberto Motterlini ◽  
Julia Gross ◽  
Marten H. Vos ◽  
Ursula Liebl
Keyword(s):  
Ligand Binding ◽  
Sensor Protein ◽  
Heme Domain ◽  
Co Sensor

scholarly journals Stationary and Time-resolved Resonance Raman Spectra of His77and Met95Mutants of the Isolated Heme Domain of a Direct Oxygen Sensor fromEscherichia coli

2002 ◽  
Vol 277 (36) ◽  
pp. 32650-32658 ◽  
Author(s):  
Akira Sato ◽  
Yukie Sasakura ◽  
Shunpei Sugiyama ◽  
Ikuko Sagami ◽  
Toru Shimizu ◽  
...  
Keyword(s):  
Raman Spectra ◽  
Oxygen Sensor ◽  
Resonance Raman ◽  
Time Resolved ◽  
Fromescherichia Coli ◽  
Heme Domain ◽  
Resonance Raman Spectra

scholarly journals Syndecan Transmembrane Domain Specifically Regulates Downstream Signaling Events of the Transmembrane Receptor Cytoplasmic Domain

2021 ◽  
Vol 22 (15) ◽  
pp. 7918
Author(s):  
Jisun Hwang ◽  
Bohee Jang ◽  
Ayoung Kim ◽  
Yejin Lee ◽  
Joonha Lee ◽  
...  
Keyword(s):  
Transmembrane Domain ◽  
Growth Factor Receptor ◽  
Cytoplasmic Domain ◽  
Nih3t3 Cells ◽  
C Elegans ◽  
Downstream Signaling ◽  
Downstream Effectors ◽  
Signaling Events

Despite the known importance of the transmembrane domain (TMD) of syndecan receptors in cell adhesion and signaling, the molecular basis for syndecan TMD function remains unknown. Using in vivo invertebrate models, we found that mammalian syndecan-2 rescued both the guidance defects in C. elegans hermaphrodite-specific neurons and the impaired development of the midline axons of Drosophila caused by the loss of endogenous syndecan. These compensatory effects, however, were reduced significantly when syndecan-2 dimerization-defective TMD mutants were introduced. To further investigate the role of the TMD, we generated a chimera, 2eTPC, comprising the TMD of syndecan-2 linked to the cytoplasmic domain of platelet-derived growth factor receptor (PDGFR). This chimera exhibited SDS-resistant dimer formation that was lost in the corresponding dimerization-defective syndecan-2 TMD mutant, 2eT(GL)PC. Moreover, 2eTPC specifically enhanced Tyr 579 and Tyr 857 phosphorylation in the PDGFR cytoplasmic domain, while the TMD mutant failed to support such phosphorylation. Finally, 2eTPC, but not 2eT(GL)PC, induced phosphorylation of Src and PI3 kinase (known downstream effectors of Tyr 579 phosphorylation) and promoted Src-mediated migration of NIH3T3 cells. Taken together, these data suggest that the TMD of a syndecan-2 specifically regulates receptor cytoplasmic domain function and subsequent downstream signaling events controlling cell behavior.

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