scholarly journals Identification and Biochemical Characterization of Unique Secretory Nucleases of the Human Enteric Pathogen,Entamoeba histolytica

2007 ◽  
Vol 282 (43) ◽  
pp. 31789-31802 ◽  
Author(s):  
Glen C. McGugan ◽  
Manju B. Joshi ◽  
Dennis M. Dwyer
Keyword(s):  
Entamoeba Histolytica ◽  
Biochemical Characterization ◽  
Enteric Pathogen

Electron probe analysis and biochemical characterization of electron-dense granules secreted by Entamoeba histolytica

1997 ◽  
Vol 85 (2) ◽  
pp. 233-242 ◽  
Author(s):  
Gloria León ◽  
Charles Fiori ◽  
Pradeep Das ◽  
Miguel Moreno ◽  
Rosalinda Tovar ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Electron Probe ◽  
Biochemical Characterization ◽  
Electron Probe Analysis ◽  
Dense Granules ◽  
Probe Analysis

scholarly journals An Alcohol Dehydrogenase 3 (ADH3) from Entamoeba histolytica Is Involved in the Detoxification of Toxic Aldehydes

2020 ◽  
Vol 8 (10) ◽  
pp. 1608
Author(s):  
Constantin König ◽  
Martin Meyer ◽  
Corinna Lender ◽  
Sarah Nehls ◽  
Tina Wallaschkowski ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Entamoeba Histolytica ◽  
Biochemical Characterization ◽  
Stop Codon ◽  
In Silico Analysis ◽  
Peptidase Activity ◽  
Stool Samples ◽  
Human Stool

Recently, a putative alcohol dehydrogenase 3, termed EhADH3B of the Entamoeba histolytica isolate HM-1:IMSS was identified, which is expressed at higher levels in non-pathogenic than in pathogenic amoebae and whose overexpression reduces the virulence of pathogenic amoebae. In an in silico analysis performed in this study, we assigned EhADH3B to a four-member ADH3 family, with ehadh3b present as a duplicate (ehadh3ba/ehadh3bb). In long-term laboratory cultures a mutation was identified at position 496 of ehadh3ba, which codes for a stop codon, which was not the case for amoebae isolated from human stool samples. When using transfectants that overexpress or silence ehadh3bb, we found no or little effect on growth, size, erythrophagocytosis, motility, hemolytic or cysteine peptidase activity. Biochemical characterization of the recombinant EhADH3Bb revealed that this protein forms a dimer containing Ni2+ or Zn2+ as a co-factor and that the enzyme converts acetaldehyde and formaldehyde in the presence of NADPH. A catalytic activity based on alcohols as substrates was not detected. Based on the results, we postulate that EhADH3Bb can reduce free acetaldehyde released by hydrolysis from bifunctional acetaldehyde/alcohol dehydrogenase-bound thiohemiacetal and that it is involved in detoxification of toxic aldehydes produced by the host or the gut microbiota.

Biochemical characterization of the DNA ligase I from Entamoeba histolytica

2010 ◽  
Vol 174 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Cesar S. Cardona-Felix ◽  
Guillermo Pastor-Palacios ◽  
Helios Cardenas ◽  
Elisa Azuara-Liceaga ◽  
Luis G. Brieba
Keyword(s):  
Entamoeba Histolytica ◽  
Biochemical Characterization ◽  
Dna Ligase ◽  
Dna Ligase I

Entamoeba histolytica: Biochemical characterization of a protein disulfide isomerase

2011 ◽  
Vol 128 (1) ◽  
pp. 76-81 ◽  
Author(s):  
Marco A. Ramos ◽  
Rosa E. Mares ◽  
Paloma D. Magaña ◽  
Israel D. Rivas ◽  
Samuel G. Meléndez-López
Keyword(s):  
Entamoeba Histolytica ◽  
Protein Disulfide Isomerase ◽  
Biochemical Characterization ◽  
Disulfide Isomerase ◽  
Protein Disulfide

Purification and Biochemical Characterization of Three Cysteine Proteases of Entamoeba histolytica with Potential Application in Epidemiologic Trials

2000 ◽  
Vol 31 (4) ◽  
pp. S49-S52
Author(s):  
Enrique González ◽  
Guadalupe Rico ◽  
Patricia Morán ◽  
Fernando Ramos ◽  
Gabriela Garcı́a ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Potential Application ◽  
Biochemical Characterization ◽  
Cysteine Proteases

Purification and biochemical characterization of a novel cysteine protease of Entamoeba histolytica

1999 ◽  
Vol 266 (1) ◽  
pp. 170-180 ◽  
Author(s):  
Sandrine Spinella ◽  
Etienne Levavasseur ◽  
Farhettin Petek ◽  
Marie C. Rigothier
Keyword(s):  
Entamoeba Histolytica ◽  
Cysteine Protease ◽  
Biochemical Characterization
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