scholarly journals Phosphatidylinositol 3-Kinase-dependent Membrane Recruitment of Rac-1 and p47phoxIs Critical for α-Platelet-derived Growth Factor Receptor-induced Production of Reactive Oxygen Species

2007 ◽  
Vol 283 (12) ◽  
pp. 7864-7876 ◽  
Author(s):  
Anselm T. Bäumer ◽  
Henrik ten Freyhaus ◽  
Heinrich Sauer ◽  
Maria Wartenberg ◽  
Kai Kappert ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Growth Factor ◽  
Growth Factor Receptor ◽  
Reactive Oxygen ◽  
Platelet Derived Growth Factor ◽  
Oxygen Species ◽  
Phosphatidylinositol 3 Kinase ◽  
Membrane Recruitment ◽  
Phosphatidylinositol 3

Exposure of differentiated airway smooth muscle cells to serum stimulates both induction of hypoxia-inducible factor-1α and airway responsiveness to ACh

2007 ◽  
Vol 293 (4) ◽  
pp. L913-L922 ◽  
Author(s):  
Georgia Chachami ◽  
Apostolia Hatziefthimiou ◽  
Panagiotis Liakos ◽  
Maria G. Ioannou ◽  
Georgios K. Koukoulis ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Hypoxia Inducible Factor ◽  
Reactive Oxygen ◽  
Serum Starvation ◽  
Airway Smooth Muscle Cells ◽  
Oxygen Species ◽  
Phosphatidylinositol 3 Kinase ◽  
Phosphatidylinositol 3

Airway smooth muscle (ASM) cells are characterized by phenotypic plasticity and can switch between differentiated and proliferative phenotypes. In rabbit tracheal ASM cells that had been differentiated in vitro by serum starvation, readdition of FBS caused initiation of proliferation and induction of nuclear and transcriptionally active hypoxia-inducible factor (HIF)-1α. In addition, FBS stimulated the induction of HIF-1α by the hypoxia mimetic cobalt. Treatment with actinomycin D, cycloheximide, the phosphatidylinositol 3-kinase inhibitors LY-294002 and wortmannin or the reactive oxygen species scavenger diphenyleneiodonium inhibited the FBS-dependent induction of HIF-1α. These data indicate that, in differentiated ASM cells, FBS upregulates HIF-1α by a transcription-, translation-, phosphatidylinositol 3-kinase-, and reactive oxygen species-dependent mechanism. Interestingly, addition of FBS and cobalt also induced HIF-1α in organ cultures of rabbit trachea strips and synergistically increased their contractile response to ACh, suggesting that HIF-1α might be implicated in airway hypercontractility.

scholarly journals Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase

FEBS Letters ◽  
2005 ◽  
Vol 579 (5) ◽  
pp. 1243-1248 ◽  
Author(s):  
Jung Hee Joo ◽  
Ho Jung Yoo ◽  
Inhwan Hwang ◽  
June Seung Lee ◽  
Kyoung Hee Nam ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Phosphatidylinositol 3 Kinase ◽  
Species Production ◽  
Phosphatidylinositol 3
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