scholarly journals A Novel Glycine Receptor β Subunit Splice Variant Predicts an Unorthodox Transmembrane Topology

2006 ◽  
Vol 282 (5) ◽  
pp. 2798-2807 ◽  
Author(s):  
Jana Oertel ◽  
Carmen Villmann ◽  
Helmut Kettenmann ◽  
Frank Kirchhoff ◽  
Cord-Michael Becker
Keyword(s):  
Splice Variant ◽  
Glycine Receptor ◽  
Β Subunit ◽  
Transmembrane Topology

Glycine receptor β–subunit gene mutation in spastic mouse associated with LINE–1 element insertion

1994 ◽  
Vol 7 (2) ◽  
pp. 136-142 ◽  
Author(s):  
Stephen F. Kingsmore ◽  
Bruno Giros ◽  
David Suh ◽  
Mark Bieniarz ◽  
Marc G. Caron ◽  
...  
Keyword(s):  
Gene Mutation ◽  
Glycine Receptor ◽  
Β Subunit ◽  
Subunit Gene ◽  
Element Insertion

scholarly journals Hydrophobic Interactions Mediate Binding of the Glycine Receptor β-Subunit to Gephyrin

2008 ◽  
Vol 72 (3) ◽  
pp. 1323-1326 ◽  
Author(s):  
Matthias Kneussel ◽  
Achim Hermann ◽  
Joachim Kirsch ◽  
Heinrich Betz
Keyword(s):  
Hydrophobic Interactions ◽  
Glycine Receptor ◽  
Β Subunit

scholarly journals Glycine receptor α4 subunit facilitates the early embryonic development in mice

Reproduction ◽  
2020 ◽  
Vol 159 (1) ◽  
pp. 41 ◽  
Author(s):  
Hirofumi Nishizono ◽  
Mohamed Darwish ◽  
Takaho A Endo ◽  
Kyosuke Uno ◽  
Hiroyuki Abe ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Litter Size ◽  
Glycine Receptor ◽  
Blastocyst Stage ◽  
Preimplantation Development ◽  
Β Subunit ◽  
Neurotransmitter Receptor ◽  
Oviduct Fluid ◽  
Fertilized Egg ◽  
Number Of Cells

Oviduct fluid is essential for the fertilization and subsequent preimplantation development. Glycine is abundant in oviduct fluid and is reported to be critical for preimplantation development of fertilized eggs in mammals. However, the mechanism by which glycine exerts its action on fertilized eggs is yet to be understood. Here we show that glycine regulates the preimplantation development of mouse fertilized eggs via glycine receptors. Among them, the alpha-4 subunit (Glra4) and the β subunit are expressed in mouse fertilized eggs, and lacking Glra4 inhibits embryonic development to the blastocyst stage, decreases the number of cells in the blastocysts and the litter size. Thus, we identify a novel function of the glycine receptor, which is considered to act mainly as a neurotransmitter receptor, as a regulator of embryonic development and our data provide new insights into the interactions between oviduct milieu and mammalian fertilized egg.

scholarly journals Glycine receptor α3K governs mobility and conductance of L/K splice variant heteropentamers

2021 ◽  
Author(s):  
Veerle Lemmens ◽  
Bart Thevelein ◽  
Svenja Kankowski ◽  
Hideaki Mizuno ◽  
Jochen C. Meier ◽  
...  
Keyword(s):  
Chronic Pain ◽  
Single Molecule ◽  
Splice Variant ◽  
Single Channel ◽  
Pain Treatment ◽  
Conflicts Of Interest ◽  
Splice Variants ◽  
Formal Analysis ◽  
Glycine Receptor ◽  
Link Type

AbstractGlycine receptors (GlyRs) are ligand-gated pentameric chloride channels in the central nervous system. GlyR-α3 is a possible target for chronic pain treatment and temporal lobe epilepsy. Alternative splicing into K or L variants determines the subcellular fate and function of GlyR-α3, yet it remains to be shown whether its different splice variants can functionally co-assemble, and what the properties of such heteropentamers would be. Here, we subjected GlyR-α3 to a combined fluorescence microscopy and electrophysiology analysis. We employ masked Pearson’s and dual-color spatiotemporal correlation analysis to prove that GlyR-α3 splice variants heteropentamerize, adopting the mobility of the K variant. Fluorescence-based single-subunit counting experiments revealed a variable and concentration ratio dependent hetero-stoichiometry. Via single-channel on-cell patch clamp we show heteropentameric conductances resemble those of the α3K splice variant. Our data are compatible with a model where α3 heteropentamerization fine-tunes mobility and activity of GlyR α3 channels, which is important to understand and tackle α3 related diseases.SummaryThe glycine receptor α3 is key to the central nervous system’s physiology and involved in chronic pain and epilepsy. In this paper, Lemmens et al. reveal and functionally characterize α3 splice variant heteropentamerization via advanced single-molecule fluorescence image analysis.DeclarationsFundingWe acknowledge the UHasselt Advanced Optical Microscopy Centre (AOMC). Prof. Em. Marcel Ameloot, the Research Foundation Flanders (FWO, project G0H3716N) and the province of Limburg (Belgium) (tUL Impuls II) are acknowledged for funding the microscopy hardware. V. Lemmens is grateful for a doctoral scholarship from the UHasselt (17DOC11BOF) and KU Leuven (C14/16/053) Special Research Funds (BOF).Conflicts of interest / competing interestsNo conflicts of interest apply.Ethics approvalNot applicableAvailability of data and materialAll data and material are available upon request.Code availabilityFluctuation imaging and co-localization analyses were performed in the software package PAM [71]. The software is available as source code, requiring MATLAB to run, or as pre-compiled standalone distributions for Windows or MacOS athttp://www.cup.uni-muenchen.de/pc/lamb/software/pam.htmlor hosted in Git repositories underhttp://www.gitlab.com/PAM-PIE/PAMandhttp://www.gitlab.com/PAM-PIE/PAMcompiled. A detailed user manual is available athttp://pam.readthedocs.io.Author contributionsConceptualization Meier J.C., Brône B. and Hendrix J.; Investigation and formal analysis Lemmens V. and Thevelein B.; Software development Hendrix J.; Writing the original draft Lemmens V., Thevelein B and Hendrix, J.; Review and editing by all authors.

Expression of glycine receptor subunits and gephyrin in single bipolar cells of the rat retina

1995 ◽  
Vol 12 (3) ◽  
pp. 501-507 ◽  
Author(s):  
Ralf Enz ◽  
Joachim Bormann
Keyword(s):  
Glycine Receptor ◽  
Bipolar Cells ◽  
Β Subunit ◽  
Rt Pcr ◽  
Patch Clamp Recording ◽  
Chain Reaction ◽  
Rat Retina ◽  
Adult Rat ◽  
Polymerase Chain ◽  
Rod Bipolar Cells

AbstractWe studied the expression of glycine receptor (GlyR) subunits and gephyrin in the adult rat retina. Reverse transcribed RNA was amplified by polymerase chain reaction (RT-PCR) with primers designed to recognize GlyR α1, α2, α3, β subunits, and gephyrin. Using RNA isolated from the whole retina, signals for all four GlyR subunits and gephyrin could be observed. In rod bipolar cells, in contrast, we detected a subset of GlyR subunits, α1 and β, and no gephyrin. Patch-clamp recording employing two subtype-specific blockers of the GlyR, picrotoxinin and cyanotriphenylborate (CTB), indicated that the GlyR in rod bipolar cells is a heteromeric protein composed of the α1 and β subunit. Moreover, the absence of detectable amounts of gephyrin mRNA suggests that the anchor protein is not required for the function of GlyRs in rod bipolar cells.

scholarly journals Novel missense mutations in the glycine receptor β subunit gene (GLRB) in startle disease

2013 ◽  
Vol 52 ◽  
pp. 137-149 ◽  
Author(s):  
Victoria M. James ◽  
Anna Bode ◽  
Seo-Kyung Chung ◽  
Jennifer L. Gill ◽  
Maartje Nielsen ◽  
...  
Keyword(s):  
Glycine Receptor ◽  
Missense Mutations ◽  
Β Subunit ◽  
Subunit Gene ◽  
Startle Disease

scholarly journals Transient neuromotor phenotype in transgenic spastic mice expressing low levels of glycine receptor β-subunit: an animal model of startle disease

2000 ◽  
Vol 12 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Lore Becker ◽  
Bettina Hartenstein ◽  
Johannes Schenkel ◽  
Jochen Kuhse ◽  
Heinrich Betz ◽  
...  
Keyword(s):  
Animal Model ◽  
Glycine Receptor ◽  
Β Subunit ◽  
Startle Disease ◽  
Low Levels

scholarly journals Identification of a gephyrin binding motif on the glycine receptor β subunit

Neuron ◽  
1995 ◽  
Vol 15 (3) ◽  
pp. 563-572 ◽  
Author(s):  
Guido Meyer ◽  
Joachim Kirsch ◽  
Heinrich Betz ◽  
Dieter Langosch
Keyword(s):  
Glycine Receptor ◽  
Binding Motif ◽  
Β Subunit

The human glycine receptor β subunit: primary structure, functional characterisation and chromosomal localisation of the human and murine genes

1996 ◽  
Vol 35 (1-2) ◽  
pp. 211-219 ◽  
Author(s):  
Cheryl A. Handford ◽  
Joseph W. Lynch ◽  
Elizabeth Baker ◽  
Graham C. Webb ◽  
Judith H. Ford ◽  
...  
Keyword(s):  
Primary Structure ◽  
Glycine Receptor ◽  
Β Subunit ◽  
Functional Characterisation ◽  
Chromosomal Localisation
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