scholarly journals Expression and Functional Characterization of the Cancer-related Serine Protease, Human Tissue Kallikrein 14

2006 ◽  
Vol 282 (4) ◽  
pp. 2405-2422 ◽  
Author(s):  
Carla A. Borgoño ◽  
Iacovos P. Michael ◽  
Julie L. V. Shaw ◽  
Liu-Ying Luo ◽  
Manik C. Ghosh ◽  
...  
Keyword(s):  
Serine Protease ◽  
Human Tissue ◽  
Functional Characterization ◽  
Tissue Kallikrein

Biochemical and functional characterization of the human tissue kallikrein 9

2017 ◽  
Vol 474 (14) ◽  
pp. 2417-2433 ◽  
Author(s):  
Panagiota S. Filippou ◽  
Sofia Farkona ◽  
Davor Brinc ◽  
Yijing Yu ◽  
Ioannis Prassas ◽  
...  
Keyword(s):  
Human Tissue ◽  
Biochemical Characterization ◽  
Functional Characterization ◽  
Squamous Carcinoma ◽  
Biochemical Properties ◽  
Tissue Kallikrein ◽  
Related Family ◽  
Squamous Carcinoma Cells

Human tissue kallikrein 9 (KLK9) is a member of the kallikrein-related family of proteases. Despite its known expression profile, much less is known about the functional roles of this protease and its implications in normal physiology and disease. We present here the first data on the biochemical characterization of KLK9, investigate parameters that affect its enzymatic activity (such as inhibitors) and provide preliminary insights into its putative substrates. We show that mature KLK9 is a glycosylated chymotrypsin-like enzyme with strong preference for tyrosine over phenylalanine at the P1 cleavage position. The enzyme activity is enhanced by Mg2+ and Ca2+, but is reversibly attenuated by Zn2+. KLK9 is inhibited in vitro by many naturally occurring or synthetic protease inhibitors. Using a combination of degradomic and substrate specificity assays, we identified candidate KLK9 substrates in two different epithelial cell lines [the non-tumorigenic human keratinocyte cells (HaCaT) and the tumorigenic tongue squamous carcinoma cells (SCC9)]. Two potential KLK9 substrates [KLK10 and midkine (MDK)] were subjected to further validation. Taken together, our data delineate some functional and biochemical properties of KLK9 for future elucidation of the role of this enzyme in health and disease.

Functional characterization of a serine protease from Crotalus durissus collilineatus highly expressed in Pichia pastoris: Comparison to its native form

2014 ◽  
Vol 229 ◽  
pp. S55-S56
Author(s):  
Johara Boldrini-França ◽  
Renata Santos Rodrigues ◽  
Ludier Kesser Santos-Silva ◽  
Dayane Lorena Naves de Souza ◽  
Mário Sérgio Rocha Gomes ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Serine Protease ◽  
Functional Characterization ◽  
Native Form ◽  
Crotalus Durissus ◽  
Crotalus Durissus Collilineatus

Functional characterization of a serine protease inhibitor modulated in the infection of the Aedes aegypti with dengue virus

Biochimie ◽  
2018 ◽  
Vol 144 ◽  
pp. 160-168 ◽  
Author(s):  
Tatiane Sanches Soares ◽  
Boris Luis Rodriguez Gonzalez ◽  
Ricardo José Soares Torquato ◽  
Francisco Jose Alves Lemos ◽  
André L. Costa-da-Silva ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Protease Inhibitor ◽  
Serine Protease ◽  
Functional Characterization ◽  
Serine Protease Inhibitor
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