scholarly journals Critical Role of Serine 465 in Isoflurane-induced Increase of Cell-surface Redistribution and Activity of Glutamate Transporter Type 3

2006 ◽  
Vol 281 (50) ◽  
pp. 38133-38138 ◽  
Author(s):  
Yueming Huang ◽  
Xiaorong Feng ◽  
Julianne J. Sando ◽  
Zhiyi Zuo
Keyword(s):  
Cell Surface ◽  
Critical Role ◽  
Glutamate Transporter ◽  
Type 3

scholarly journals A critical role of glutamate transporter type 3 in the learning and memory of mice

2014 ◽  
Vol 114 ◽  
pp. 70-80 ◽  
Author(s):  
Zhi Wang ◽  
Sang-Hon Park ◽  
Huijuan Zhao ◽  
Shuling Peng ◽  
Zhiyi Zuo
Keyword(s):  
Learning And Memory ◽  
Critical Role ◽  
Glutamate Transporter ◽  
Type 3

scholarly journals Critical Role of S465 in Protein Kinase C-Increased Rat Glutamate Transporter Type 3 Activity

2009 ◽  
Vol 119 (9) ◽  
pp. 1419-1428 ◽  
Author(s):  
Hee Jung Baik ◽  
Yueming Huang ◽  
Jacqueline M. Washington ◽  
Zhiyi Zuo
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Critical Role ◽  
Glutamate Transporter ◽  
Kinase C ◽  
Type 3

Critical role of the C-terminal segment in the maturation and export to the cell surface of the homopentameric alpha7-5HT3A receptor

2004 ◽  
Vol 20 (8) ◽  
pp. 2022-2030 ◽  
Author(s):  
S. Pons ◽  
J. Sallette ◽  
J. P. Bourgeois ◽  
A. Taly ◽  
J. P. Changeux ◽  
...  
Keyword(s):  
Cell Surface ◽  
Critical Role ◽  
Terminal Segment

scholarly journals Inhibition of isoflurane-induced increase of cell-surface redistribution and activity of glutamate transporter type 3 by serine 465 sequence-specific peptides

2011 ◽  
Vol 655 (1-3) ◽  
pp. 16-22 ◽  
Author(s):  
Yueming Huang ◽  
Liaoliao Li ◽  
Jacqueline M. Washington ◽  
Xuebing Xu ◽  
Julianne J. Sando ◽  
...  
Keyword(s):  
Cell Surface ◽  
Glutamate Transporter ◽  
Type 3

scholarly journals Critical Role of Types 2 and 3 Deiodinases in the Negative Regulation of Gene Expression by T3 in the Mouse Cerebral Cortex

Endocrinology ◽  
2012 ◽  
Vol 153 (6) ◽  
pp. 2919-2928 ◽  
Author(s):  
Arturo Hernandez ◽  
Beatriz Morte ◽  
Mónica M. Belinchón ◽  
Ainhoa Ceballos ◽  
Juan Bernal
Keyword(s):  
Gene Expression ◽  
Cerebral Cortex ◽  
Thyroid Hormone ◽  
Critical Role ◽  
Regulation Of Gene Expression ◽  
Control Of Gene Expression ◽  
High Doses ◽  
And Function ◽  
Type 3

Thyroid hormones regulate brain development and function through the control of gene expression, mediated by binding of T3 to nuclear receptors. Brain T3 concentration is tightly controlled by homeostatic mechanisms regulating transport and metabolism of T4 and T3. We have examined the role of the inactivating enzyme type 3 deiodinase (D3) in the regulation of 43 thyroid hormone-dependent genes in the cerebral cortex of 30-d-old mice. D3 inactivation increased slightly the expression of two of 22 positively regulated genes and significantly decreased the expression of seven of 21 negatively regulated genes. Administration of high doses of T3 led to significant changes in the expression of 12 positive genes and three negative genes in wild-type mice. The response to T3 treatment was enhanced in D3-deficient mice, both in the number of genes and in the amplitude of the response, demonstrating the role of D3 in modulating T3 action. Comparison of the effects on gene expression observed in D3 deficiency with those in hypothyroidism, hyperthyroidism, and type 2 deiodinase (D2) deficiency revealed that the negative genes are more sensitive to D2 and D3 deficiencies than the positive genes. This observation indicates that, in normal physiological conditions, D2 and D3 play critical roles in maintaining local T3 concentrations within a very narrow range. It also suggests that negatively and positively regulated genes do not have the same physiological significance or that their regulation by thyroid hormone obeys different paradigms at the molecular or cellular levels.

scholarly journals A cationic motif in Engrailed-2 homeoprotein controls its internalization via selective cell-surface glycosaminoglycans interactions

2021 ◽  
Author(s):  
Sebastien CARDON ◽  
Gerard BOLBACH ◽  
Yadira P HERVIS ◽  
Chrystel LOPIN-BON ◽  
Jean-Claude JACQUINET ◽  
...  
Keyword(s):  
Cell Surface ◽  
Critical Role ◽  
Amino Acid Level ◽  
Cationic Protein ◽  
Intrinsically Disordered ◽  
Structure And Dynamics ◽  
Internalization Mechanism ◽  
Initial Interaction ◽  
Basic Region

Engrailed-2 (En2) is a transcription factor that possesses as most homeoproteins the unique and intriguing property to transfer from cell to cell through unconventional pathways. The internalization mechanism of this cationic protein is far from being fully understood and is proposed to require an initial interaction with cell-surface glycosaminoglycans (GAGs). To decipher the role of GAGs in the recognition of En2 at the cell surface, we have quantified the internalization of the homeodomain region in cell lines that differ in their content in cell-surface GAGs. The binding specificity to GAGs and the influence of this interaction on the structure and dynamics of En2 was also investigated at the amino acid level. Our results show that a high-affinity GAG-binding hexadecapeptide (RKPKKKNPNKEDKRPR) located upstream of the homeodomain controls internalization efficiency of En2 through selective interactions with highly-sulfated GAGs of heparan sulfate type. Our data underline the functional importance of the intrinsically disordered basic region that precedes the prominent internalization domain in En2, and demonstrate the critical role of GAGs as an entry gate for En2, finely tuning its capacity to internalize into cells.

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