scholarly journals Structural Characterization of a K-antigen Capsular Polysaccharide Essential for Normal Symbiotic Infection inRhizobiumsp. NGR234

2006 ◽  
Vol 281 (39) ◽  
pp. 28981-28992 ◽  
Author(s):  
Antoine J.-L. Le Quéré ◽  
William J. Deakin ◽  
Christel Schmeisser ◽  
Russell W. Carlson ◽  
Wolfgang R. Streit ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Capsular Polysaccharide ◽  
K Antigen

Structural characterization of the antigenic capsular polysaccharide and lipopolysaccharide O-chain produced byActinobacillus pleuropneumoniaeserotype 15

2005 ◽  
Vol 83 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Malcolm B Perry ◽  
Leann L MacLean ◽  
Evgeny Vinogradov
Keyword(s):  
Molecular Mass ◽  
Key Words ◽  
Structural Characterization ◽  
Capsular Polysaccharide ◽  
Actinobacillus Pleuropneumoniae ◽  
High Molecular Mass ◽  
O Antigen ◽  
Phosphate Diester ◽  
Nmr Methods

The specific capsular polysaccharide produced by Actinobacillus pleuropneumoniae serotype 15 was determined to be a high-molecular-mass polymer having [α]D+ 69° (water) and composed of a linear backbone of phosphate diester linked disaccharide units of 2-acetamido-2-deoxy-D-glucose (D-GlcNAc) and 2-acetamido-2-deoxy-D-galactose (D-GalNAc) residues (1:1). Thirty percent of the D-GalNAc residues were substituted at O-4 by β-D-galactopyranose (β-D-Galp) residues. Through the application of chemical and NMR methods, the capsule, which defines the serotype specificity of the bacterium, was found to have the structure                        [Formula: see text]The O-polysaccharide (O-PS) component of the A. pleuro pneumoniae serotype 15 lipopolysaccharide (LPS) was characterized as a linear unbranched polymer of repeating pentasaccharide units composed of D-glucose (2 parts) and D-galactose (3 parts), shown to have the structure[Formula: see text]The O-PS was chemically identical with the O-antigen previously identified in the LPSs produced by A. pleuro pneumoniae serotypes 3 and 8. Key words: Capsule, lipopolysaccharide, Actinobacillus pleuropneumoniae serotype 15.

scholarly journals Structural characterization of the lipopolysaccharide O-antigen and capsular polysaccharide of Vibrio ordalii serotype O : 2

1998 ◽  
Vol 253 (1) ◽  
pp. 319-327 ◽  
Author(s):  
Irina Sadovskaya ◽  
Jean-Robert Brisson ◽  
Nam Huan Khieu ◽  
Lucy M. Mutharia ◽  
Eleonora Altman
Keyword(s):  
Structural Characterization ◽  
Capsular Polysaccharide ◽  
O Antigen ◽  
Serotype O ◽  
Vibrio Ordalii

scholarly journals Erratum to: Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H

2017 ◽  
Vol 110 (11) ◽  
pp. 1389-1389
Author(s):  
Angela Casillo ◽  
Jonas Ståhle ◽  
Ermenegilda Parrilli ◽  
Filomena Sannino ◽  
Daniel E. Mitchell ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Capsular Polysaccharide ◽  
Colwellia Psychrerythraea

scholarly journals Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H

2017 ◽  
Vol 110 (11) ◽  
pp. 1377-1387 ◽  
Author(s):  
Angela Casillo ◽  
Jonas Ståhle ◽  
Ermenegilda Parrilli ◽  
Filomena Sannino ◽  
Daniel E. Mitchell ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Capsular Polysaccharide ◽  
Colwellia Psychrerythraea

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

Structural characterization of the PPIase domain of FKBP51, a cochaperone of human Hsp90

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
A Bracher ◽  
C Kozany ◽  
AK Thost ◽  
F Hausch
Keyword(s):  
Structural Characterization ◽  
Ppiase Domain
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