scholarly journals HCELL Is the Major E- and L-selectin Ligand Expressed on LS174T Colon Carcinoma Cells

2006 ◽  
Vol 281 (20) ◽  
pp. 13899-13905 ◽  
Author(s):  
Monica M. Burdick ◽  
Julia T. Chu ◽  
Samuel Godar ◽  
Robert Sackstein
Keyword(s):  
Colon Carcinoma ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Selectin Ligand

scholarly journals CD44 on LS174T Colon Carcinoma Cells Possesses E-Selectin Ligand Activity

2005 ◽  
Vol 65 (13) ◽  
pp. 5812-5817 ◽  
Author(s):  
William D. Hanley ◽  
Monica M. Burdick ◽  
Konstantinos Konstantopoulos ◽  
Robert Sackstein
Keyword(s):  
Colon Carcinoma ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Selectin Ligand ◽  
Ligand Activity

scholarly journals Podocalyxin-like protein is an E-/L-selectin ligand on colon carcinoma cells: comparative biochemical properties of selectin ligands in host and tumor cells

2009 ◽  
Vol 296 (3) ◽  
pp. C505-C513 ◽  
Author(s):  
Susan N. Thomas ◽  
Ronald L. Schnaar ◽  
Konstantinos Konstantopoulos
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Colon Carcinoma ◽  
Carcinoma Cells ◽  
Host Cells ◽  
High Endothelial Venules ◽  
Colon Carcinoma Cells ◽  
Selectin Ligands ◽  
Selectin Ligand ◽  
Selectin Binding

Selectins facilitate metastasis and tumor cell arrest in the microvasculature by mediating binding of selectin-expressing host cells to ligands on tumor cells. We recently identified CD44 variant isoforms as functional P-, but not E-/L-, selectin ligands on colon carcinoma cells. Furthermore, a ∼180-kDa sialofucosylated glycoprotein(s) mediated selectin binding in CD44-knockdown cells. Using immunoaffinity chromatography and tandem mass spectrometry, we identify podocalyxin-like protein (PCLP) as an alternative selectin ligand. Blot rolling and cell-free flow-based adhesion assays disclose that PCLP on LS174T colon carcinoma cells possesses E-/L-, but not P-, selectin binding activity. The selectin-binding determinants on LS174T PCLP are non-MECA-79-reactive sialofucosylated structures displayed on O-linked glycans, distinct from the MECA-79-reactive O-glycans on PCLP expressed by high endothelial venules, which is an L-selectin ligand. PCLP on CD44-knockdown LS174T cells exhibits higher HECA-452 immunoreactivity than PCLP on wild-type cells, suggesting that PCLP functions as an alternative acceptor for selectin-binding glycans. The enhanced expression of HECA-452 reactivity on PCLP from CD44-knockdown cells correlates with the increased avidity of PCLP for E- but not L-selectin. The novel finding that PCLP is an E-/L-selectin ligand on carcinoma cells offers a unifying perspective on the apparent enhanced metastatic potential associated with tumor cell PCLP overexpression and the role of selectins in metastasis.

scholarly journals Selectin Ligand Expression Regulates the Initial Vascular Interactions of Colon Carcinoma Cells

2007 ◽  
Vol 282 (6) ◽  
pp. 3433-3441 ◽  
Author(s):  
Susan L. Napier ◽  
Zachary R. Healy ◽  
Ronald L. Schnaar ◽  
Konstantinos Konstantopoulos
Keyword(s):  
Colon Carcinoma ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Ligand Expression ◽  
Selectin Ligand

The dual role of CD44 as a functional P-selectin ligand and fibrin receptor in colon carcinoma cell adhesion

2008 ◽  
Vol 294 (4) ◽  
pp. C907-C916 ◽  
Author(s):  
Christina S. Alves ◽  
Monica M. Burdick ◽  
Susan N. Thomas ◽  
Parag Pawar ◽  
Konstantinos Konstantopoulos
Keyword(s):  
Colon Carcinoma ◽  
Carcinoma Cell ◽  
Metastatic Potential ◽  
Carcinoma Cells ◽  
Fibrinogen Receptor ◽  
Colon Carcinoma Cells ◽  
Selectin Ligand ◽  
And Control ◽  
Kinetics Of

Selectins and fibrin(ogen) play key roles in the hematogenous dissemination of tumor cells, and especially of colon carcinomas. However, the fibrin(ogen) receptor(s) on colon carcinoma cells has yet to be defined along with its relative capacity to bind fibrinogen versus fibrin under flow. Moreover, the functional P-selectin ligand has yet to be validated using intact platelets rather than purified selectin substrates. Using human CD44-knockdown and control LS174T cells, we demonstrate the pivotal involvement of CD44 in the P-selectin-mediated binding to platelets in shear flow. Quantitative comparisons of the binding kinetics of LS174T versus P-selectin glycoprotein ligand-1 (PSGL-1)-expressing THP-1 cells to activated platelets reveal that the relative avidity of P-selectin-CD44 binding is more than sevenfold lower than that of P-selectin-PSGL-1 interaction. Using CD44-knockdown LS174T cells and microspheres coated with CD44 immunoprecipitated from control LS174T cells, and purified fibrin(ogen) as substrate, we provide the first direct evidence that CD44 also acts as the major fibrin, but not fibrinogen, receptor on LS174T colon carcinoma cells. Interestingly, binding of plasma fibrin to CD44 on the colon carcinoma cell surface interferes with the P-selectin-CD44 molecular interaction and diminishes platelet-LS174T heteroaggregation in the high shear regime. Cumulatively, our data offer a novel perspective on the apparent metastatic potential associated with CD44 overexpression on colon carcinoma cells and the critical roles of P-selectin and fibrin(ogen) in metastatic spread and provide a rational basis for the design of new therapeutic strategies to impede metastasis.

The influence of extracts from Rubus caesius leaves on human colon carcinoma cells cultured in vitro

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
R Paduch ◽  
M Tomczyk ◽  
A Wiater ◽  
A Dudek ◽  
M Pleszczynska ◽  
...  
Keyword(s):  
Colon Carcinoma ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma ◽  
Cells Cultured

Nitric Oxide Exposure of CC531 Rat Colon Carcinoma Cells Induces γ-glutamyltransferase which May Counteract Glutathione Depletion and Cell Death

2003 ◽  
Vol 37 (1) ◽  
pp. 99-107 ◽  
Author(s):  
Nils-Erik Huseby ◽  
Nana Asare ◽  
Silje Wetting ◽  
Idun Merete Mikkelsen ◽  
Bente Mortensen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Colon Carcinoma ◽  
Carcinoma Cells ◽  
Glutathione Depletion ◽  
Rat Colon ◽  
Colon Carcinoma Cells
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