scholarly journals Transgenic Mice Expressing Dominant-negative Osmotic-response Element-binding Protein (OREBP) in Lens Exhibit Fiber Cell Elongation Defect Associated with Increased DNA Breaks

2005 ◽  
Vol 280 (20) ◽  
pp. 19986-19991 ◽  
Author(s):  
Yu Wang ◽  
Ben C. B. Ko ◽  
James Y. Yang ◽  
Terrence T. L. Lam ◽  
Zhirong Jiang ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cell Elongation ◽  
Binding Protein ◽  
Dominant Negative ◽  
Fiber Cell ◽  
Dna Breaks ◽  
Response Element ◽  
Osmotic Response ◽  
Element Binding Protein

scholarly journals CREB (cAMP response element binding protein) and C/EBPα (CCAAT/enhancer binding protein) are required for the superstimulation of phosphoenolpyruvate carboxykinase gene transcription by adenoviral E1a and cAMP

2000 ◽  
Vol 352 (2) ◽  
pp. 335-342 ◽  
Author(s):  
John M. ROUTES ◽  
Lillester A. COLTON ◽  
Sharon RYAN ◽  
Dwight J. KLEMM
Keyword(s):  
Gene Transcription ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Binding Protein ◽  
Gene Promoter ◽  
Camp Response Element Binding ◽  
Dominant Negative ◽  
Camp Response Element ◽  
Response Element ◽  
Element Binding Protein ◽  
In Cells

In the present study, we observed superstimulated levels of cAMP-stimulated transcription from the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter in cells infected with wild-type adenovirus expressing 12S and 13S E1a proteins, or in cells expressing 13S E1a alone. cAMP-stimulated transcription was inhibited in cells expressing only 12S E1a, but slightly elevated in cells expressing E1a proteins with mutations in conserved regions 1 or 2, leading us to conclude that the superstimulation was mediated by conserved region 3 of 13S E1a. E1a failed to enhance cAMP-stimulated transcription from promoters containing mutations that abolish binding by cAMP response element binding protein (CREB) or CCAAT/enhancer binding proteins (C/EBPs). This result was supported by experiments in which expression of dominant-negative CREB and/or C/EBP proteins repressed E1a- and cAMP-stimulated transcription from the PEPCK gene promoter. In reconstitution experiments using a Gal4-responsive promoter, E1a enhanced cAMP-stimulated transcription when chimaeric Gal4–CREB and Gal4–C/EBPα were co-expressed. Phosphorylation of CREB on serine-133 was stimulated in cells treated with dibutyryl cAMP, whereas phosphorylation of C/EBPα was increased by E1a expression. Our data support a model in which cAMP agonists increase CREB activity and stimulate PEPCK gene transcription, a process that is enhanced by E1a through the phosphorylation of C/EBPα.

Purification, Identification, and Characterization of an Osmotic Response Element Binding Protein

2000 ◽  
Vol 270 (1) ◽  
pp. 52-61 ◽  
Author(s):  
Ben C.B. Ko ◽  
Christoph W. Turck ◽  
Karen W.Y. Lee ◽  
Yinqing Yang ◽  
Stephen S.M. Chung
Keyword(s):  
Binding Protein ◽  
Response Element ◽  
Osmotic Response ◽  
Element Binding Protein ◽  
Identification And Characterization

scholarly journals Osmotic Response Element-binding Protein (OREBP) Is an Essential Regulator of the Urine Concentrating Mechanism

2004 ◽  
Vol 279 (46) ◽  
pp. 48048-48054 ◽  
Author(s):  
Amy K. M. Lam ◽  
Ben C. B. Ko ◽  
Sidney Tam ◽  
Ryan Morris ◽  
James Y. Yang ◽  
...  
Keyword(s):  
Binding Protein ◽  
Response Element ◽  
Concentrating Mechanism ◽  
Osmotic Response ◽  
Element Binding Protein ◽  
Urine Concentrating Mechanism
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