scholarly journals Peroxisome Proliferator-activated Receptor-binding Protein Null Mutation Results in Defective Mammary Gland Development

2005 ◽  
Vol 280 (11) ◽  
pp. 10766-10773 ◽  
Author(s):  
Yuzhi Jia ◽  
Chao Qi ◽  
Zhongyi Zhang ◽  
Yiwei Tony Zhu ◽  
Sambasiva M. Rao ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Receptor Binding ◽  
Mammary Gland Development ◽  
Binding Protein ◽  
Null Mutation ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Gland Development ◽  
Receptor Binding Protein

scholarly journals Peroxisome-proliferator-activated receptor-binding protein (PBP) is essential for the growth of active Notch4-immortalized mammary epithelial cells by activating SOX10 expression

2009 ◽  
Vol 425 (2) ◽  
pp. 435-444 ◽  
Author(s):  
Yiwei Tony Zhu ◽  
Yuzhi Jia ◽  
Liping Hu ◽  
Chao Qi ◽  
Megana K. Prasad ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Receptor Binding ◽  
Binding Protein ◽  
Active Form ◽  
Mammary Epithelial ◽  
Peroxisome Proliferator ◽  
Mammary Cells ◽  
Peroxisome Proliferator Activated Receptor ◽  
Sox10 Expression ◽  
Receptor Binding Protein

PBP (peroxisome-proliferator-activated receptor-binding protein) [Med1 (mediator 1)/TRAP220 (thyroid-hormone-receptor-associated protein 220)] is essential for mammary gland development. We established a mammary epithelial cell line with a genotype of PBPLoxP/LoxP by expressing an active form of Notch4. Null mutation of PBP caused severe growth inhibition of the Notch4-immortalized mammary cells. We found that truncated PBP without the two LXXLL motifs could reverse the growth inhibition due to the deficiency of endogenous PBP, indicating that signalling through nuclear receptors is unlikely to be responsible for the growth inhibition as the result of PBP deficiency. Loss of PBP expression was shown to completely ablate the expression of SOX10 [Sry-related HMG (high-mobility group) box gene 10]. The re-expression of SOX10 was capable of reversing the growth inhibition due to PBP deficiency, whereas suppressed expression of SOX10 inhibited the growth of Notch4-immortalized mammary cells. Further studies revealed PBP is directly recruited to the enhancer of the SOX10 gene, indicating that SOX10 is a direct target gene of PBP. We conclude that PBP is essential for the growth of Notch4-immortalized mammary cells by activating SOX10 expression, providing a potential molecular mechanism through which PBP regulates the growth of mammary stem/progenitor cells.

Cold-inducible RNA binding protein in mouse mammary gland development

2016 ◽  
Vol 48 (6) ◽  
pp. 577-587 ◽  
Author(s):  
Daniel A. Lujan ◽  
Selina Garcia ◽  
Jennifer Vanderhoof ◽  
Joshua Sifuentes ◽  
Yekaterina Brandt ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mammary Gland Development ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mouse Mammary Gland ◽  
Gland Development
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