scholarly journals Analysis of Double-stranded RNA-induced Apoptosis Pathways UsingInterferon-response Noninducible Small Interfering RNA Expression VectorLibrary*

2005 ◽  
Vol 280 (27) ◽  
pp. 25687-25696 ◽  
Author(s):  
Sahohime Matsumoto ◽  
Makoto Miyagishi ◽  
Hideo Akashi ◽  
Ryozo Nagai ◽  
Kazunari Taira
Keyword(s):  
Small Interfering Rna ◽  
Rna Expression ◽  
Double Stranded Rna ◽  
Apoptosis Pathways ◽  
Induced Apoptosis ◽  
Interfering Rna

scholarly journals Interferon-αEnhances 5′-Deoxy-5-Fluorouridine-Induced Apoptosis by ERK-Dependant Upregulation of Thymidine Phosphorylase

2013 ◽  
Vol 2013 ◽  
pp. 1-8
Author(s):  
Yike Zhu ◽  
Ling Xu ◽  
Yibo Fan ◽  
Ce Li ◽  
Ye Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Small Interfering Rna ◽  
Thymidine Phosphorylase ◽  
Antitumor Efficacy ◽  
Erk Signaling ◽  
Gastric Cancer Cells ◽  
High Concentrations ◽  
Induced Apoptosis ◽  
Interfering Rna

5-Florouracil (5-FU) is the basic agent used in the treatment of gastric cancer. Capecitabine, a prodrug of 5-FU, displays increased antitumor efficacy compared with 5-FU in the clinic.5′-Deoxy-5-fluorouracil (5′-DFUR), the metabolite of capecitabine, is converted to 5-FU by the enzyme thymidine phosphorylase (TP), which is present at high concentrations in human tumors. In this study, we investigated the effect of interferon-α(IFN-α) on the sensitivity of gastric cancer cells to treatment with5′-DFUR and its relationship with TP expression. Preincubation of gastric cancer cells with IFN-αenhanced5′-DFUR-induced apoptosis via IFN-α-mediated upregulation of TP. The depletion of TP with small interfering RNA (siRNA) obviously inhibited IFN-α-induced upregulation of TP expression and thus prevented apoptosis induced by IFN-αand5′-DFUR. Treatment with IFN-αand combined IFN-αand5′-DFUR treatment were also associated with concomitant activation of ERK signaling. Treatment with the ERK inhibitor PD98059 or depletion of ERK with siRNA partially reversed IFN-α-induced upregulation of TP expression, thus partially preventing apoptosis induced by IFN-αand5′-DFUR. Taken together, our study shows that IFN-αenhanced5′-DFUR-induced apoptosis in gastric cancer cells by upregulation of TP expression, which is partially regulated by activation of ERK signaling.

scholarly journals Single small-interfering RNA expression vector for silencing multiple transforming growth factor-  pathway components

2005 ◽  
Vol 33 (15) ◽  
pp. e131-e131 ◽  
Author(s):  
A. Jazag ◽  
F. Kanai ◽  
H. Ijichi ◽  
K. Tateishi ◽  
T. Ikenoue ◽  
...  
Keyword(s):  
Growth Factor ◽  
Small Interfering Rna ◽  
Expression Vector ◽  
Transforming Growth Factor ◽  
Rna Expression ◽  
Interfering Rna
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