scholarly journals Role of Hepatocyte Nuclear Factors in Transcriptional Regulation of Male-specificCYP2A2

2004 ◽  
Vol 280 (5) ◽  
pp. 3259-3268 ◽  
Author(s):  
Christopher A. Wiwi ◽  
David J. Waxman
Keyword(s):  
Transcriptional Regulation ◽  
Nuclear Factors ◽  
Hepatocyte Nuclear Factors

scholarly journals Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 466
Author(s):  
Marie-Christine Carpentier ◽  
Cécile Bousquet-Antonelli ◽  
Rémy Merret
Keyword(s):  
Gene Expression ◽  
Quality Control ◽  
Transcriptional Regulation ◽  
High Throughput ◽  
Library Preparation ◽  
Working Day ◽  
Set Up ◽  
Post Transcriptional Regulation ◽  
Commercial Kit

The recent development of high-throughput technologies based on RNA sequencing has allowed a better description of the role of post-transcriptional regulation in gene expression. In particular, the development of degradome approaches based on the capture of 5′monophosphate decay intermediates allows the discovery of a new decay pathway called co-translational mRNA decay. Thanks to these approaches, ribosome dynamics could now be revealed by analysis of 5′P reads accumulation. However, library preparation could be difficult to set-up for non-specialists. Here, we present a fast and efficient 5′P degradome library preparation for Arabidopsis samples. Our protocol was designed without commercial kit and gel purification and can be easily done in one working day. We demonstrated the robustness and the reproducibility of our protocol. Finally, we present the bioinformatic reads-outs necessary to assess library quality control.

Role of the Genetic Polymorphisms in the 5'-Flanking Region for Transcriptional Regulation of the Human CYP2E1 Gene

2007 ◽  
Vol 31 (s1) ◽  
pp. S36-S42 ◽  
Author(s):  
Takayuki Uchimoto ◽  
Sakae Itoga ◽  
Masahiko Nezu ◽  
Masahiko Sunaga ◽  
Takeshi Tomonaga ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Genetic Polymorphisms ◽  
Cyp2e1 Gene ◽  
Flanking Region

scholarly journals Glutamate-dependent transcriptional regulation of GLAST: role of PKC

2004 ◽  
Vol 91 (1) ◽  
pp. 200-209 ◽  
Author(s):  
Esther Lopez-Bayghen ◽  
Arturo Ortega
Keyword(s):  
Transcriptional Regulation

scholarly journals Sigma-1 receptor mediates cocaine-induced transcriptional regulation by recruiting chromatin-remodeling factors at the nuclear envelope

2015 ◽  
Vol 112 (47) ◽  
pp. E6562-E6570 ◽  
Author(s):  
Shang-Yi A. Tsai ◽  
Jian-Ying Chuang ◽  
Meng-Shan Tsai ◽  
Xiao-fei Wang ◽  
Zheng-Xiong Xi ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Nuclear Envelope ◽  
Chromatin Remodeling ◽  
Behavioral Sensitization ◽  
Specific Protein ◽  
Cellular Regulation ◽  
Sigma 1 Receptor ◽  
Withdrawal Treatment ◽  
Sigma 1

The sigma-1 receptor (Sig-1R) chaperone at the endoplasmic reticulum (ER) plays important roles in cellular regulation. Here we found a new function of Sig-1R, in that it translocates from the ER to the nuclear envelope (NE) to recruit chromatin-remodeling molecules and regulate the gene transcription thereof. Sig-1Rs mainly reside at the ER–mitochondrion interface. However, on stimulation by agonists such as cocaine, Sig-1Rs translocate from ER to the NE, where Sig-1Rs bind NE protein emerin and recruit chromatin-remodeling molecules, including lamin A/C, barrier-to-autointegration factor (BAF), and histone deacetylase (HDAC), to form a complex with the gene repressor specific protein 3 (Sp3). Knockdown of Sig-1Rs attenuates the complex formation. Cocaine was found to suppress the gene expression of monoamine oxidase B (MAOB) in the brain of wild-type but not Sig-1R knockout mouse. A single dose of cocaine (20 mg/kg) in rats suppresses the level of MAOB at nuclear accumbens without affecting the level of dopamine transporter. Daily injections of cocaine in rats caused behavioral sensitization. Withdrawal from cocaine in cocaine-sensitized rats induced an apparent time-dependent rebound of the MAOB protein level to about 200% over control on day 14 after withdrawal. Treatment of cocaine-withdrawn rats with the MAOB inhibitor deprenyl completely alleviated the behavioral sensitization to cocaine. Our results demonstrate a role of Sig-1R in transcriptional regulation and suggest cocaine may work through this newly discovered genomic action to achieve its addictive action. Results also suggest the MAOB inhibitor deprenyl as a therapeutic agent to block certain actions of cocaine during withdrawal.

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