scholarly journals The SANT Domain of Human MI-ER1 Interacts with Sp1 to Interfere with GC Box Recognition and Repress Transcription from Its Own Promoter

2004 ◽  
Vol 279 (27) ◽  
pp. 28009-28016 ◽  
Author(s):  
Zhihu Ding ◽  
Laura L. Gillespie ◽  
F. Corinne Mercer ◽  
Gary D. Paterno
Keyword(s):  
Sant Domain ◽  
Gc Box

scholarly journals Regulation of GC box activity by 8-oxoguanine

Redox Biology ◽  
2021 ◽  
pp. 101997
Author(s):  
Nadine Müller ◽  
Andriy Khobta
Keyword(s):  
Gc Box

Transcriptional Activation Domain of Human BTEB2, A GC Box-Binding Factor

1997 ◽  
Vol 121 (2) ◽  
pp. 389-396 ◽  
Author(s):  
S. Kojima ◽  
A. Kobayashi ◽  
O. Gotoh ◽  
Y. Ohkuma ◽  
Y. Fujii-Kuriyama ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Activation Domain ◽  
Transcriptional Activation Domain ◽  
Binding Factor ◽  
Gc Box

scholarly journals In vivo competition of delta-crystallin gene expression by DNA fragments containing a GC box.

1986 ◽  
Vol 6 (11) ◽  
pp. 4130-4132 ◽  
Author(s):  
S Hayashi ◽  
H Kondoh
Keyword(s):  
Gene Expression ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Dna Fragments ◽  
Specific Expression ◽  
Mouse Cells ◽  
Gc Box ◽  
Simplex Virus

Expression of the chicken delta-crystallin gene 1 injected into the nuclei of mouse cells is lens specific. Coinjection of GC box-containing DNA fragments from delta-crystallin, simian virus 40 early, and herpes simplex virus type 1 tk promoters effectively suppressed delta-crystallin expression in the lens, but coinjection with DNA fragments not containing the GC box did not. This suppression was likely due to the competition of an Sp1-like transcription factor(s) and indicates involvement of the apparently ubiquitous factor(s) in the tissue-specific expression of the delta-crystallin gene.

scholarly journals Regulation of protamine gene expression in an in vitro homologous system.

1996 ◽  
Vol 43 (2) ◽  
pp. 369-377 ◽  
Author(s):  
J M Jankowski ◽  
P D Cannon ◽  
F Van der Hoorn ◽  
L D Wasilewska ◽  
N C Wong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulatory Elements ◽  
In Vitro Transcription ◽  
Tata Box ◽  
Transcription System ◽  
Proposed Model ◽  
Binding Factor ◽  
Gc Box ◽  
Responsive Element

An in vitro transcription system from the trout testis nuclei was developed to study trout protamine gene expression. The protamine promoter contains, among others, two regulatory elements: 1) a cAMP-responsive element or CRE element (TGACGTCA) which is present in position 5' to TATA box, and 2) GC box (CCGCCC) which is present in position 3' to TATA box. The removal of the CRE-binding protein by titration (by the addition of appropriate oligonucleotides to the incubation mixture) resulted in a decrease in transcription of the protamine gene. These results were confirmed by experiments in which the pure CRE-binding factor (TPBP1) was used, as well as by those where a stimulatory effect of cAMP on protamine promoter transcription was observed. On the other hand, addition of oligonucleotides containing the GC-box sequence enhanced the protamine gene transcription indicating that the protein (Sp1 like) which binds to this sequence acts as a repressor of protamine gene expression. These results confirm the previously proposed model which suggested that the GC box played a role in negative regulation of the protamine gene expression. Involvement of some other factors in this process was also discussed.

Octamer and SPH motifs in the U1 enhancer cooperate to activate U1 RNA gene expression

1990 ◽  
Vol 10 (1) ◽  
pp. 341-352
Author(s):  
K A Roebuck ◽  
D P Szeto ◽  
K P Green ◽  
Q N Fan ◽  
W E Stumph
Keyword(s):  
Dna Sequence ◽  
Functional Activity ◽  
Stable Complex ◽  
Small Nuclear Rna ◽  
Base Pairs ◽  
Rna Transcription ◽  
Octamer Motif ◽  
Chicken Tissue ◽  
Nuclear Rna ◽  
Gc Box

The transcriptional enhancer of a chicken U1 small nuclear RNA gene has been shown to extend over approximately 50 base pairs of DNA sequence located 180 to 230 base pairs upstream of the U1 transcription initiation site. It is composed of multiple functional motifs, including a GC box, an octamer motif, and a novel SPH motif. The contributions of these three distinct sequence motifs to enhancer function were studied with an oocyte expression assay. Under noncompetitive conditions in oocytes, the SPH motif is capable of stimulating U1 RNA transcription in the absence of the other functional motifs, whereas the octamer motif by itself lacks this ability. However, to form a transcription complex that is stable to challenge by a second competing small nuclear RNA transcription unit, both the octamer and SPH motifs are required. The GC box, although required for full enhancer activity, is not essential for stable complex formation in oocytes. Site-directed mutagenesis was used to study the DNA sequence requirements of the SPH motif. Functional activity of the SPH motif is spread throughout a 24-base-pair region 3' of the octamer but is particularly dependent upon sequences near an SphI restriction site located at the center of the SPH motif. Using embryonic chicken tissue as a source material, we identified and partially purified a factor, termed SBF, that binds sequence specifically to the SPH motif of the U1 enhancer. The ability of this factor to recognize and bind to mutant enhancer DNA fragments in vitro correlates with the functional activity of the corresponding enhancer sequences in vivo.

Perinatal regulation of the ClC-2 chloride channel in lung is mediated by Sp1 and Sp3

1999 ◽  
Vol 276 (4) ◽  
pp. L614-L624 ◽  
Author(s):  
Shijian Chu ◽  
Carol J. Blaisdell ◽  
Min-Zhi M. Liu ◽  
Pamela L. Zeitlin
Keyword(s):  
Chloride Channel ◽  
Fetal Lung ◽  
Airway Disease ◽  
Perinatal Period ◽  
Lung Epithelial Cells ◽  
Normal Lung ◽  
Mobility Shift ◽  
Channel Expression ◽  
Gc Box ◽  
Electrophoretic Mobility Shift Assays

Mechanisms responsible for regulation of pulmonary epithelial chloride-channel expression in the perinatal period are under investigation to better understand normal lung development and airway disease pathogenesis. The ClC-2 epithelial chloride channel is regulated by changes in pH and volume and is most abundant in lung during fetal development. In this study, we identify and sequence the ClC-2 promoter, which is GC rich and lacks a TATA box. By construction of a series of promoter-luciferase constructs, a 67-bp GC box-containing sequence in the promoter is shown to be critical to ClC-2 expression in primary and immortalized fetal lung epithelial cells. Electrophoretic mobility shift assays and antibody supershifts demonstrate that the Sp1 and Sp3 transcription factors are expressed in fetal lung nuclei and interact with the GC box sequences in the promoter. Immunoblotting techniques demonstrate that Sp1 and Sp3 are perinatally downregulated in the lung with the same temporal sequence as ClC-2 downregulation. This work suggests that Sp1 and Sp3 activate ClC-2 gene transcription and that reduction in Sp1 and Sp3 at birth explains perinatal downregulation of ClC-2 in the lung.

Developmentally and regionally regulated alterations of octamer- and GC-box-binding activities during the postnatal development of mouse cerebellum

1991 ◽  
Vol 61 (2) ◽  
pp. 161-168 ◽  
Author(s):  
Hiroaki Sakurai ◽  
Kaoru Kikuchi ◽  
Tomofusa Tsuchiya ◽  
Hiroshi Kanazawa ◽  
Masaaki Tsuda
Keyword(s):  
Postnatal Development ◽  
Mouse Cerebellum ◽  
Gc Box

scholarly journals Metal-responsive elements act as positive modulators of human metallothionein-IIA enhancer activity.

1987 ◽  
Vol 7 (2) ◽  
pp. 606-613 ◽  
Author(s):  
M Karin ◽  
A Haslinger ◽  
A Heguy ◽  
T Dietlin ◽  
T Cooke
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Mutational Analysis ◽  
Enhancer Activity ◽  
Enhancer Element ◽  
Basal Activity ◽  
Before And After ◽  
Positive Modulator ◽  
Gc Box

The human metallothionein IIA (hMT-IIA) gene contains two enhancer elements whose activity is induced by heavy-metal ions such as Cd2+. To determine the nature of the relationship between the metal-responsive elements and the element(s) responsible for the basal activity of the enhancers, the basal-level enhancer element(s), the hMT-IIA enhancers were subjected to mutational analysis. We show that deletion of the metal-responsive elements had no effect on the basal activity of the enhancer but prevented further induction by Cd2+. On the other hand, replacement of the basal-level enhancer element with linker DNA led to inactivation of the enhancer both before and after treatment with Cd2+. Therefore, the metal-responsive elements seems to act as a positive modulator of enhancer function in the presence of heavy-metal ions. In addition to the two enhancers, the hMT-IIA promoter contained one other element, the GC box, required for its basal expression. Unlike deletion of the basal-level enhancer element, replacement of the GC box with linker DNA had no effect on the ability of the promoter to be induced by Cd2+.

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