scholarly journals Characterization of OSR1, a Member of the Mammalian Ste20p/Germinal Center Kinase Subfamily

2004 ◽  
Vol 279 (12) ◽  
pp. 11129-11136 ◽  
Author(s):  
Wei Chen ◽  
Mustafa Yazicioglu ◽  
Melanie H. Cobb
Keyword(s):  
Germinal Center ◽  
Kinase Subfamily

scholarly journals Cloning and Characterization of Cell Adhesion Kinase β, a Novel Protein-tyrosine Kinase of the Focal Adhesion Kinase Subfamily

1995 ◽  
Vol 270 (36) ◽  
pp. 21206-21219 ◽  
Author(s):  
Hiroko Sasaki ◽  
Kazuko Nagura ◽  
Masaho Ishino ◽  
Hirotoshi Tobioka ◽  
Kiyoshi Kotani ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Tyrosine Kinase ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Protein Tyrosine Kinase ◽  
Protein Tyrosine ◽  
Kinase Subfamily ◽  
Novel Protein

scholarly journals PHGDH is required for germinal center formation and is a therapeutic target in MYC-driven lymphoma

2021 ◽  
Author(s):  
Annalisa D'Avola ◽  
Nathalie Legrave ◽  
Mylene Tajan ◽  
Probir Chakravarty ◽  
Ryan Shearer ◽  
...  
Keyword(s):  
B Cell ◽  
Germinal Center ◽  
Cell Activation ◽  
Cellular Proliferation ◽  
B Cell Lymphoma ◽  
B Cell Activation ◽  
Germinal Center Reaction ◽  
Germinal Center B Cell

The synthesis of serine from glucose is a key metabolic pathway supporting cellular proliferation in healthy and malignant cells. Despite this, the role that this aspect of metabolism plays in germinal center biology and pathology is not known. Here, we performed a comprehensive characterization of the role of the serine synthesis pathway in germinal center B cells and lymphomas derived from these cells. We demonstrate that upregulation of a functional serine synthesis pathway is a metabolic hallmark of B-cell activation and the germinal center reaction. Inhibition of phosphoglycerate dehydrogenase (PHGDH), the first and rate limiting enzyme in this pathway, leads to defective germinal formation and impaired high-affinity antibody production. In addition, overexpression of enzymes involved in serine synthesis is a characteristic of germinal center B-cell derived lymphomas, with high levels of expression being predictive of reduced overall survival in diffuse large B cell lymphoma. Inhibition of PHGDH induces apoptosis in lymphoma cells reducing disease progression. These findings establish PHGDH as a critical player in humoral immunity and a clinically relevant target in lymphoma.

Characterization of germinal center dendritic cells in follicular lymphoma

1999 ◽  
Vol 27 (12) ◽  
pp. 1768-1775 ◽  
Author(s):  
Nathalie Renard ◽  
Jenny Valladeau ◽  
Clarisse Barthelemy ◽  
Patricia Ribeiro ◽  
Françoise Berger ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Follicular Lymphoma ◽  
Germinal Center

scholarly journals Identification and Characterization of a Novel Ste20/Germinal Center Kinase-related Kinase, Polyploidy-associated Protein Kinase

2003 ◽  
Vol 278 (15) ◽  
pp. 13520-13530 ◽  
Author(s):  
Kazuo Nishigaki ◽  
Delores Thompson ◽  
Takashi Yugawa ◽  
Karen Rulli ◽  
Charlotte Hanson ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Germinal Center ◽  
Identification And Characterization

RNA-Binding Protein VICKZ Is Expressed in a Germinal Center Associated Pattern among Lymphoma Subtypes.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 1909-1909
Author(s):  
Yasodha Natkunam ◽  
Gilad Vainer ◽  
Shuchun Zhao ◽  
Gail Amir ◽  
Eli Pikarsky ◽  
...  
Keyword(s):  
B Cell ◽  
Germinal Center ◽  
Rna Binding ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Recent Effort ◽  
Large B Cell Lymphoma ◽  
Differential Expression Pattern ◽  
Large B Cell

Abstract Recent effort in the molecular characterization of diffuse large B-cell lymphoma (DLBCL) has led to the recognition that patients with DLBCL of germinal center origin exhibit a better overall survival. Thus, identification and characterization of markers of germinal center derivation are of importance in dissecting prognostic subclasses of DLBCL. The VICKZ (Vg1 RBP/Vera, IMP1, 2, 3, CRD-BP, KOC, ZBP-1) family members are RNA-binding proteins that recognize specific RNA targets and have been implicated in diverse cellular functions including cell polarity, migration, proliferation and tumorigenesis/metastasis. We generated an antibody that recognizes all three isoforms of VICKZ protein and characterized its expression in normal lymphoid tissue and in lymphoma subtypes. In normal tonsils, VICKZ protein showed a germinal center-specific pattern of expression with staining localized to the cytoplasm. Among 868 non-Hodgkin and Hodgkin lymphomas tested by immunohistochemistry on tissue microarrays, staining for VICKZ protein was present in 76% (126/165) of follicular lymphoma, 78% (155/200) of DLBCL, 90% (9/10) of mediastinal large B-cell lymphoma, and 100% (2/2) of Burkitt lymphoma. A subset of mantle cell lymphoma (11%, 2/19), extranodal (8%, 2/25), and nodal (20%, 1/5) marginal zone lymphoma and lymphoblastic lymphoma (25%, 4/13), showed VICKZ staining. The majority of lymphocyte predominant Hodgkin (92%, 12/13) and classical Hodgkin (94%, 101/108) lymphoma were found to be positive. Among T cell lymphoma, anaplastic large cell lymphoma were positive (75%, 6/9). Additional work is in progress to correlate VICKZ protein expression with other germinal center markers such as HGAL, BCL6 and CD10 as well as with prognostic subclasses of DLBCL. The differential expression pattern of VICKZ protein in lymphoma subtypes suggests a potential utility for VICKZ in the identification of subgroups of DLBCL associated with different prognoses.

scholarly journals Identification and Characterization of Rp5: A Novel Gene Differentially Expressed in Germinal Center B-Cells

1999 ◽  
Vol 45 (4, Part 2 of 2) ◽  
pp. 152A-152A
Author(s):  
Jason Shohet ◽  
Kimble Frazer ◽  
Yong-Jun Lui ◽  
J Donald Capra ◽  
Jacques Banchereau ◽  
...  
Keyword(s):  
B Cells ◽  
Germinal Center ◽  
Differentially Expressed ◽  
Novel Gene ◽  
Identification And Characterization ◽  
Germinal Center B Cells

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

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