scholarly journals Identification of a Functionally Critical Protein Kinase C Phosphorylation Residue of Cardiac Troponin T

2003 ◽  
Vol 278 (37) ◽  
pp. 35135-35144 ◽  
Author(s):  
Marius P. Sumandea ◽  
W. Glen Pyle ◽  
Tomoyoshi Kobayashi ◽  
Pieter P. de Tombe ◽  
R. John Solaro
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Cardiac Troponin T ◽  
Kinase C

scholarly journals Protein kinase C phosphorylation of cardiac troponin T decreases Ca2+-dependent actomyosin MgATPase activity and troponin T binding to tropomyosin-F-actin complex

1992 ◽  
Vol 288 (1) ◽  
pp. 123-129 ◽  
Author(s):  
T A Noland ◽  
J F Kuo
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Cardiac Troponin ◽  
Thin Filament ◽  
Phorbol Esters ◽  
Troponin T ◽  
Cardiac Troponin T ◽  
Kinase C ◽  
Inotropic Responses ◽  
Mgatpase Activity

Effects of phosphorylation of bovine cardiac troponin T (TnT) by protein kinase C on the Ca(2+)-stimulated MgATPase activity of reconstituted actomyosin complex and the binding of TnT to tropomyosin(Tm)-F-actin were investigated. The Ca(2+)-stimulated MgATPase of actomyosin containing phosphorylated TnT (1.8 mol of P/mol), compared with that containing unphosphorylated TnT, was decreased by up to 48%. Phosphorylation of TnT also decreased (up to 48%) its maximum binding to Tm-F-actin, which was accompanied by a decrease (up to 3.5-fold) in its apparent binding affinity. The findings indicate that the effects of phosphorylated TnT in decreasing actomyosin MgATPase might be secondary to its decreased interactions with the other components of the thin filament, representing a new mechanism underlying the negative inotropic responses of various cardiac preparations to protein kinase C-activating phorbol esters.

scholarly journals Phosphorylation Specificities of Protein Kinase C Isozymes for Bovine Cardiac Troponin I and Troponin T and Sites within These Proteins and Regulation of Myofilament Properties

1996 ◽  
Vol 271 (38) ◽  
pp. 23277-23283 ◽  
Author(s):  
Nathan M. Jideama ◽  
Thomas A. Noland ◽  
Robert L. Raynor ◽  
Gerard C. Blobe ◽  
Doriano Fabbro ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Troponin T ◽  
Cardiac Troponin I ◽  
Kinase C
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