scholarly journals Transcription Factor GATA-4 Is Activated by Phosphorylation of Serine 261 via the cAMP/Protein Kinase A Signaling Pathway in Gonadal Cells

2003 ◽  
Vol 278 (24) ◽  
pp. 22128-22135 ◽  
Author(s):  
Jacques J. Tremblay ◽  
Robert S. Viger
Keyword(s):  
Transcription Factor ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Signaling Pathway ◽  
Kinase A

scholarly journals CD9 regulates transcription factor GCM1 and ERVWE1 expression through the cAMP/protein kinase A signaling pathway

Reproduction ◽  
2009 ◽  
Vol 138 (6) ◽  
pp. 945-951 ◽  
Author(s):  
Yoshikage Muroi ◽  
Toshihiro Sakurai ◽  
Akira Hanashi ◽  
Kentaro Kubota ◽  
Kentaro Nagaoka ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Protein Kinase ◽  
Membrane Protein ◽  
Protein Kinase A ◽  
Signaling Pathway ◽  
Endogenous Retrovirus ◽  
Regulatory Mechanisms ◽  
Human Endogenous Retrovirus ◽  
Envelope Gene ◽  
Kinase A

ERVWE1 (SYNCYTIN-1), a membrane protein originating from the envelope gene of human endogenous retrovirus-W (HERV-W), mediates the fusion of mononucleated cytotrophoblasts into multinucleated syncytiotrophoblast. Though ERVWE1 has been characterized since its discovery, regulatory mechanisms associated with ERVWE1 expression have not been firmly established. We hypothesized that membrane protein CD9, involved in cell–cell fusion of fertilization and myogenesis, could be involved in the regulation ofERVWE1gene expression. In this study, regulatory mechanisms of ERVWE1 expression were studied using human choriocarcinoma BeWo cells. Forskolin is an activator of adenylate cyclase, which increased CD9 and ERVWE1 expression. The increase in CD9 expression was inhibited by a protein kinase A (PKA) inhibitor, Rp-cAMPS. These results indicate that CD9 expression is regulated by the cAMP/PKA signaling pathway. Overexpression ofCD9increased expression levels of ERVWE1 as well as GCM1 (hGCMa), which is a transcription factor known to activateERVWE1gene transcription. However, high ERVWE1 expression induced byCD9overexpression did not result in the increase in chorionic gonadotropin, beta polypeptide production. Moreover,CD9-induced increase inERVWE1andGCM1expressions were inhibited by Rp-cAMPS. These results suggest that CD9 increases GCM1 expression via the cAMP/PKA signaling pathway, resulting in the increase in ERVWE1 expression.

scholarly journals Protein Kinase A Signaling Pathway Regulates Transcriptional Activity of SAF-1 by Unmasking Its DNA-binding Domains

2003 ◽  
Vol 278 (25) ◽  
pp. 22586-22595 ◽  
Author(s):  
Alpana Ray ◽  
Papiya Ray ◽  
Nicole Guthrie ◽  
Arvind Shakya ◽  
Deepak Kumar ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Dna Binding ◽  
Protein Kinase A ◽  
Signaling Pathway ◽  
Transcriptional Activity ◽  
Dna Binding Domains ◽  
Binding Domains ◽  
Kinase A
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