scholarly journals The Werner Syndrome Protein Stimulates DNA Polymerase β Strand Displacement Synthesis via Its Helicase Activity

2003 ◽  
Vol 278 (25) ◽  
pp. 22686-22695 ◽  
Author(s):  
Jeanine A. Harrigan ◽  
Patricia L. Opresko ◽  
Cayetano von Kobbe ◽  
Padmini S. Kedar ◽  
Rajendra Prasad ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Werner Syndrome ◽  
Helicase Activity ◽  
Strand Displacement ◽  
Dna Polymerase Β ◽  
Werner Syndrome Protein ◽  
Syndrome Protein

scholarly journals The Werner Syndrome Helicase Coordinates Sequential Strand Displacement and FEN1-Mediated Flap Cleavage during Polymerase δ Elongation

2016 ◽  
Vol 37 (3) ◽  
Author(s):  
Baomin Li ◽  
Sita Reddy ◽  
Lucio Comai
Keyword(s):  
Dna Polymerase ◽  
Werner Syndrome ◽  
Physiological Role ◽  
Cooperative Interaction ◽  
Strand Displacement ◽  
Winged Helix ◽  
Werner Syndrome Protein ◽  
Syndrome Protein ◽  
Lagging Strand

ABSTRACT The Werner syndrome protein (WRN) suppresses the loss of telomeres replicated by lagging-strand synthesis by a yet to be defined mechanism. Here, we show that whereas either WRN or the Bloom syndrome helicase (BLM) stimulates DNA polymerase δ progression across telomeric G-rich repeats, only WRN promotes sequential strand displacement synthesis and FEN1 cleavage, a critical step in Okazaki fragment maturation, at these sequences. Helicase activity, as well as the conserved winged-helix (WH) motif and the helicase and RNase D C-terminal (HRDC) domain play important but distinct roles in this process. Remarkably, WRN also influences the formation of FEN1 cleavage products during strand displacement on a nontelomeric substrate, suggesting that WRN recruitment and cooperative interaction with FEN1 during lagging-strand synthesis may serve to regulate sequential strand displacement and flap cleavage at other genomic sites. These findings define a biochemical context for the physiological role of WRN in maintaining genetic stability.

scholarly journals Enhancement of Human DNA Polymerase η Activity and Fidelity Is Dependent Upon a Bipartite Interaction with the Werner Syndrome Protein

2012 ◽  
Vol 287 (50) ◽  
pp. 42312-42323 ◽  
Author(s):  
Leena Maddukuri ◽  
Amit Ketkar ◽  
Sarah Eddy ◽  
Maroof K. Zafar ◽  
Wezley C. Griffin ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Werner Syndrome ◽  
Human Dna ◽  
Werner Syndrome Protein ◽  
Syndrome Protein

scholarly journals The Werner syndrome protein limits the error-prone 8-oxo-dG lesion bypass activity of human DNA polymerase kappa

2014 ◽  
Vol 42 (19) ◽  
pp. 12027-12040 ◽  
Author(s):  
Leena Maddukuri ◽  
Amit Ketkar ◽  
Sarah Eddy ◽  
Maroof K. Zafar ◽  
Robert L. Eoff
Keyword(s):  
Dna Polymerase ◽  
Werner Syndrome ◽  
Lesion Bypass ◽  
Human Dna ◽  
Werner Syndrome Protein ◽  
Syndrome Protein
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