scholarly journals Transcriptional Regulation of the Cytosolic Chaperonin θ Subunit Gene,Cctq, by Ets Domain Transcription Factors Elk-1, Sap-1a, and Net in the Absence of Serum Response Factor

2003 ◽  
Vol 278 (33) ◽  
pp. 30642-30651 ◽  
Author(s):  
Yuji Yamazaki ◽  
Hiroshi Kubota ◽  
Masami Nozaki ◽  
Kazuhiro Nagata
Keyword(s):  
Transcriptional Regulation ◽  
Transcription Factors ◽  
Serum Response Factor ◽  
Response Factor ◽  
Subunit Gene ◽  
Ets Domain ◽  
Serum Response

scholarly journals The transcription factors Elk-1 and serum response factor interact by direct protein-protein contacts mediated by a short region of Elk-1.

1994 ◽  
Vol 14 (5) ◽  
pp. 3283-3291 ◽  
Author(s):  
P Shore ◽  
A D Sharrocks
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Protein Interactions ◽  
Serum Response Factor ◽  
Specific Protein ◽  
Response Factor ◽  
Protein Protein Interactions ◽  
Amino Acid Peptide ◽  
Necessary And Sufficient ◽  
Serum Response

Transcriptional induction of the c-fos gene in response to epidermal growth factor stimulation is mediated in part by a ternary nucleoprotein complex within the promoter consisting of serum response factor (SRF), p62TCF/Elk-1 and the serum response element (SRE). Both SRF and p62TCF/Elk-1 contact the DNA and bind in a cooperative manner to the SRE. In this study, we demonstrate that SRF and Elk-1 interact directly in the absence of the SRE. A 30-amino-acid peptide from Elk-1 (B-box) is both necessary and sufficient to mediate protein-protein contacts with SRF. Moreover, the Elk-1 B-box is necessary to enable SRF-dependent binding of an alternative ETS domain (from the transcription factor PU.1) to the c-fos SRE. Mutations in either the Elk-1 B-box or the C-terminal half of the SRF DNA-binding domain (coreSRF) which show reduced ability to form ternary complexes also show greatly reduced protein-protein interactions in the absence of the SRE. Our results clearly demonstrate that direct protein-protein interactions between the transcription factors Elk-1 and SRF, in addition to DNA contacts, contribute to the formation of a ternary complex on the c-fos SRE. We discuss the wider applicability of our results in describing specific protein-protein interactions between short well-defined transcription factor domains.

Functional Involvement of Serum Response Factor in the Transcriptional Regulation of Caldesmon Gene

1998 ◽  
Vol 242 (2) ◽  
pp. 429-435 ◽  
Author(s):  
Takuya Momiyama ◽  
Ken'ichiro Hayashi ◽  
Hideto Obata ◽  
Yoshihiro Chimori ◽  
Toshirou Nishida ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Serum Response Factor ◽  
Response Factor ◽  
Functional Involvement ◽  
Serum Response

scholarly journals Contribution of serum response factor and myocardin to transcriptional regulation of smoothelins

2006 ◽  
Vol 70 (1) ◽  
pp. 136-145 ◽  
Author(s):  
S RENSEN ◽  
P NIESSEN ◽  
X LONG ◽  
P DOEVENDANS ◽  
J MIANO ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Serum Response Factor ◽  
Response Factor ◽  
Serum Response

scholarly journals Interaction of Transcription Factors with Serum Response Factor

1998 ◽  
Vol 273 (17) ◽  
pp. 10506-10514 ◽  
Author(s):  
Yan Ling ◽  
Adam G. West ◽  
E. Claire Roberts ◽  
Jeremy H. Lakey ◽  
Andrew D. Sharrocks
Keyword(s):  
Transcription Factors ◽  
Serum Response Factor ◽  
Response Factor ◽  
Serum Response

scholarly journals Calcium activates serum response factor-dependent transcription by a Ras- and Elk-1-independent mechanism that involves a Ca2+/calmodulin-dependent kinase.

1995 ◽  
Vol 15 (7) ◽  
pp. 3672-3684 ◽  
Author(s):  
C K Miranti ◽  
D D Ginty ◽  
G Huang ◽  
T Chatila ◽  
M E Greenberg
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Growth Factors ◽  
Serum Response Factor ◽  
Active Form ◽  
Response Factor ◽  
Transcriptional Responses ◽  
Pheochromocytoma Cell ◽  
Independent Mechanism ◽  
Serum Response

Enhanced levels of cytoplasmic Ca2+ due to membrane depolarization with elevated levels of KCl or exposure to the Ca2+ ionophore ionomycin stimulate serum response element (SRE)-dependent transcription in the pheochromocytoma cell line PC12. By using altered binding specificity mutants of transcription factors that bind to the SRE, it was demonstrated that in contrast to treatment with purified growth factors, such as nerve growth factor, the serum response factor (SRF), but not Elk-1, mediates Ca(2+)-regulated SRE-dependent transcription. Enhanced levels of cytoplasmic Ca2+ were found to trigger SRE-dependent transcription via a Ras-independent signaling pathway that appears to involve a Ca2+/calmodulin-dependent kinase (CaMK). Overexpression of a constitutively active form of CaMKIV stimulated SRF-dependent transcription. Taken together, these findings indicate that SRF is a versatile transcription factor that, when bound to the SRE, can function by distinct mechanisms and can mediate transcriptional responses to both CaMK- and Ras-dependent signaling pathways.

scholarly journals SrfB, a member of the Serum Response Factor family of transcription factors, regulates starvation response and early development in Dictyostelium

2008 ◽  
Vol 316 (2) ◽  
pp. 260-274 ◽  
Author(s):  
María Galardi-Castilla ◽  
Barbara Pergolizzi ◽  
Gareth Bloomfield ◽  
Jason Skelton ◽  
Al Ivens ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Early Development ◽  
Serum Response Factor ◽  
Response Factor ◽  
Starvation Response ◽  
Serum Response
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