scholarly journals High-affinity, Non-sequence-specific RNA Binding by the Open Reading Frame 1 (ORF1) Protein from Long Interspersed Nuclear Element 1 (LINE-1)

2002 ◽  
Vol 278 (10) ◽  
pp. 8112-8117 ◽  
Author(s):  
Vladimir O. Kolosha ◽  
Sandra L. Martin
Keyword(s):  
Rna Binding ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Orf1 Protein ◽  
High Affinity ◽  
Long Interspersed Nuclear Element ◽  
Open Reading Frame 1

Discovery of the Long Interspersed Nuclear Element-1 activation product [Open Reading Frame-1 (ORF1) protein] in human blood

2018 ◽  
Vol 487 ◽  
pp. 228-232 ◽  
Author(s):  
Keivan Hosseinnejad ◽  
Tyler Yin ◽  
Jeremy T. Gaskins ◽  
James L. Bailen ◽  
Saeed A. Jortani
Keyword(s):  
Human Blood ◽  
Open Reading Frame ◽  
Activation Product ◽  
Reading Frame ◽  
Orf1 Protein ◽  
Long Interspersed Nuclear Element ◽  
Open Reading Frame 1

scholarly journals Sequence and structure of mtr, an amino acid transport gene of Neurospora crassa

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 644-651 ◽  
Author(s):  
Kenneth Koo ◽  
W. Dorsey Stuart
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Neurospora Crassa ◽  
Rna Binding ◽  
Signal Sequence ◽  
Average Length ◽  
Open Reading Frame ◽  
Mrna Transcript ◽  
S1 Nuclease ◽  
Reading Frame

The gene product of the mtr locus of Neurospora crassa is required for the transport of neutral aliphatic and aromatic amino acids via the N system. We have previously cloned three cosmids containing Neurospora DNA that complement the mtr-6(r) mutant allele. The cloned DNAs were tightly linked to restriction fragment length polymorphisms that flank the mtr locus. A 2.9-kbp fragment from one cosmid was subcloned and found to complement the mtr-6(r) allele. Here we report the sequence of the fragment that hybridized to a poly(A)+ mRNA transcript of about 2300 nucleotides. We have identified an 845-bp open reading frame (ORF) having a 59-bp intron as the potential mtr ORF. S1 nuclease analysis of the transcript confirmed the transcript size and the presence of the intron. A second open reading frame was found upstream in the same reading frame as the mtr ORF and appears to be present in the mRNA transcript. The mtr ORF is predicted to encode a 261 amino acid polypeptide with a molecular mass of 28 613 Da. The proposed polypeptide exhibits six potential α-helical transmembrane domains with an average length of 23 amino acids, does not have a signal sequence, and contains amino acid sequence homologous to an RNA binding motif.Key words: sequence, membranes, ribonucleoprotein.

The Drosophila suppressor of sable gene encodes a polypeptide with regions similar to those of RNA-binding proteins

1991 ◽  
Vol 11 (2) ◽  
pp. 894-905
Author(s):  
R A Voelker ◽  
W Gibson ◽  
J P Graves ◽  
J F Sterling ◽  
M T Eisenberg
Keyword(s):  
Rna Processing ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Open Reading Frame ◽  
In Vitro Translation ◽  
Rna Recognition Motif ◽  
Reading Frame ◽  
Cdna Sequences ◽  
Suppressor Of Sable

The nucleotide sequence of the Drosophila melanogaster suppressor of sable [su(s)] gene has been determined. Comparison of genomic and cDNA sequences indicates that an approximately 7,860-nucleotide primary transcript is processed into an approximately 5-kb message, expressed during all stages of the life cycle, that contains an open reading frame capable of encoding a 1,322-amino-acid protein of approximately 150 kDa. The putative protein contains an RNA recognition motif-like region and a highly charged arginine-, lysine-, serine-, aspartic or glutamic acid-rich region that is similar to a region contained in several RNA-processing proteins. In vitro translation of in vitro-transcribed RNA from a complete cDNA yields a product whose size agrees with the size predicted by the open reading frame. Antisera against su(s) fusion proteins recognize the in vitro-translated protein and detect a protein of identical size in the nuclear fractions from tissue culture cells and embryos. The protein is also present in smaller amounts in cytoplasmic fractions of embryos. That the su(s) protein has regions similar in structure to RNA-processing protein is consistent with its known role in affecting the transcript levels of those alleles that it suppresses.

scholarly journals Quasispecies of TT Virus (TTV) with Sequence Divergence in Hypervariable Regions of the Capsid Protein in Chronic TTV Infection

1999 ◽  
Vol 73 (11) ◽  
pp. 9604-9608 ◽  
Author(s):  
Tsutomu Nishizawa ◽  
Hiroaki Okamoto ◽  
Fumio Tsuda ◽  
Tatsuya Aikawa ◽  
Yoshiki Sugai ◽  
...  
Keyword(s):  
Amino Acids ◽  
Capsid Protein ◽  
Immune Surveillance ◽  
Sequence Divergence ◽  
Open Reading Frame ◽  
Amino Acid Substitutions ◽  
Reading Frame ◽  
Tt Virus ◽  
Hypervariable Regions ◽  
Open Reading Frame 1

ABSTRACT Three hypervariable regions were identified in a central portion of open reading frame 1 of TT virus DNA, which codes for a putative capsid protein of 770 amino acids. TT virus circulates as quasispecies, with many amino acid substitutions in hypervariable regions, to evade immune surveillance of the hosts and to establish a persistent infection.

DNA vaccination of pigs with open reading frame 1–7 of PRRS virus

Vaccine ◽  
2004 ◽  
Vol 22 (27-28) ◽  
pp. 3628-3641 ◽  
Author(s):  
Annette Malene Barfoed ◽  
Merete Blixenkrone-Møller ◽  
Merethe Holm Jensen ◽  
Anette Bøtner ◽  
Søren Kamstrup
Keyword(s):  
Dna Vaccination ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Prrs Virus ◽  
Open Reading Frame 1

The open reading frame 1-encoded (‘36K’) protein of Carnation Italian ringspot virus localizes to mitochondria

2001 ◽  
Vol 82 (1) ◽  
pp. 29-34 ◽  
Author(s):  
L. Rubino ◽  
F. Weber-Lotfi ◽  
A. Dietrich ◽  
C. Stussi-Garaud ◽  
M. Russo
Keyword(s):  
Outer Membrane ◽  
Fluorescent Protein ◽  
Amorphous Material ◽  
Subcellular Fractionation ◽  
Open Reading Frame ◽  
Ringspot Virus ◽  
Mitochondrial Outer Membrane ◽  
Intermembrane Space ◽  
Reading Frame ◽  
Open Reading Frame 1

The localization of the 36 kDa (‘36K’) protein encoded by open reading frame 1 of Carnation Italian ringspot virus was studied in infected cells and in cells transiently expressing the 36K protein fused to green fluorescent protein (GFP). Subcellular fractionation demonstrated that the 36K protein accumulated in fractions containing mostly mitochondria. Fluorescence microscopy of transiently transformed cells showed that the 36K–GFP fusion protein accumulated in structures which could be stained with the mitochondrial-specific dye MitoTracker. However, these structures were larger than normal mitochondria and were irregular in shape and distribution in the cytoplasm. Electron microscopy showed severe alterations of mitochondria, which were often clumped. The stroma was more electron-opaque, the cristae were irregularly shaped, the intermembrane space was enlarged and the outer membrane was covered with an electron-dense amorphous material whose nature could not be determined. The organelle-targeted 36K protein seems to promote the overgrowth of the mitochondrial outer membrane.

Development of a Self-Cloning System for Actinomadura verrucosospora and Identification of Polyketide Synthase Genes Essential for Production of the Angucyclic Antibiotic Pradimicin

1999 ◽  
Vol 65 (6) ◽  
pp. 2703-2709 ◽  
Author(s):  
Tohru Dairi ◽  
Yoshimitsu Hamano ◽  
Tamotsu Furumai ◽  
Toshikazu Oki
Keyword(s):  
Nucleotide Sequence ◽  
Polyketide Synthase ◽  
Selectable Marker ◽  
Plasmid Vector ◽  
Open Reading Frame ◽  
Antibiotic Biosynthesis ◽  
Nucleotide Sequence Analysis ◽  
Reading Frame ◽  
Cloning System ◽  
Open Reading Frame 1

ABSTRACT A self-cloning system for Actinomadura verrucosospora, a producer of the angucyclic antibiotic pradimicin A (PRM A), has been developed. The system is based on reproducible and reliable protoplasting and regeneration conditions for A. verrucosospora and a novel plasmid vector that consists of a replicon from a newly found Actinomadura plasmid and a selectable marker cloned from the Actinomadurastrain. The system has an efficiency of more than 105CFU/microgram of DNA. Using this system, we have cloned and identified the polyketide synthase (PKS) genes essential for PRM A biosynthesis from A. verrucosospora. Nucleotide sequence analysis of the 3.5-kb SalI-SphI fragment showed that ketosynthase subunits (open reading frame 1 [ORF1] and ORF2) of the essential PKS genes have strong similarities (59 to 89%) to those for angucyclic antibiotic biosynthesis.

Correlation of long interspersed element-1 open reading frame 1 and c-Met proto-oncogene protein expression in ovarian cancer

2019 ◽  
Vol 41 (11) ◽  
pp. 1293-1299 ◽  
Author(s):  
Eun-Ji Ko ◽  
Young Lim Oh ◽  
Heung Yeol Kim ◽  
Wan Kyu Eo ◽  
Hongbae Kim ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Protein Expression ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Long Interspersed Element ◽  
Oncogene Protein ◽  
Open Reading Frame 1

scholarly journals Differential translational initiation of lbp mRNA is caused by a 5′ upstream open reading frame 1

FEBS Letters ◽  
1997 ◽  
Vol 411 (2-3) ◽  
pp. 245-250 ◽  
Author(s):  
Maria Mittag ◽  
Christoph Eckerskorn ◽  
Kerstin Strupat ◽  
J.Woodland Hastings
Keyword(s):  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Translational Initiation ◽  
Reading Frame ◽  
Open Reading Frame 1
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