scholarly journals The C-terminal Domain of the Largest Subunit of RNA Polymerase II Is Required for Stationary Phase Entry and Functionally Interacts with the Ras/PKA Signaling Pathway

2002 ◽  
Vol 277 (22) ◽  
pp. 19488-19497 ◽  
Author(s):  
Susie C. Howard ◽  
Yelena V. Budovskaya ◽  
Ya-Wen Chang ◽  
Paul K. Herman
Keyword(s):  
Rna Polymerase ◽  
Stationary Phase ◽  
Rna Polymerase Ii ◽  
Signaling Pathway ◽  
Terminal Domain ◽  
Phase Entry

scholarly journals The Ras/PKA Signaling Pathway of Saccharomyces cerevisiae Exhibits a Functional Interaction With the Sin4p Complex of the RNA Polymerase II Holoenzyme

Genetics ◽  
2001 ◽  
Vol 159 (1) ◽  
pp. 77-89 ◽  
Author(s):  
Susie C Howard ◽  
Ya-Wen Chang ◽  
Yelena V Budovskaya ◽  
Paul K Herman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Polymerase ◽  
Stationary Phase ◽  
Rna Polymerase Ii ◽  
Signaling Pathway ◽  
Resting State ◽  
Normal Growth ◽  
Nutrient Deprivation ◽  
Ras Signaling ◽  
Rna Polymerase Ii Holoenzyme

Abstract Saccharomyces cerevisiae cells enter into the G0-like resting state, stationary phase, in response to specific types of nutrient limitation. We have initiated a genetic analysis of this resting state and have identified a collection of rye mutants that exhibit a defective transcriptional response to nutrient deprivation. These transcriptional defects appear to disrupt the control of normal growth because the rye mutants are unable to enter into a normal stationary phase upon nutrient deprivation. In this study, we examined the mutants in the rye1 complementation group and found that rye1 mutants were also defective for stationary phase entry. Interestingly, the RYE1 gene was found to be identical to SIN4, a gene that encodes a component of the yeast Mediator complex within the RNA polymerase II holoenzyme. Moreover, mutations that affected proteins within the Sin4p module of the Mediator exhibited specific genetic interactions with the Ras protein signaling pathway. For example, mutations that elevated the levels of Ras signaling, like RAS2val19, were synthetic lethal with sin4. In all, our data suggest that specific proteins within the RNA polymerase II holoenzyme might be targets of signal transduction pathways that are responsible for coordinating gene expression with cell growth.

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