scholarly journals The FabR (YijC) Transcription Factor Regulates Unsaturated Fatty Acid Biosynthesis inEscherichia coli

2002 ◽  
Vol 277 (18) ◽  
pp. 15558-15565 ◽  
Author(s):  
Yong-Mei Zhang ◽  
Hedia Marrakchi ◽  
Charles O. Rock
Keyword(s):  
Transcription Factor ◽  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Inescherichia Coli ◽  
Acid Biosynthesis

scholarly journals Functions of the Clostridium acetobutylicium FabF and FabZ proteins in unsaturated fatty acid biosynthesis

2009 ◽  
Vol 9 (1) ◽  
pp. 119 ◽  
Author(s):  
Lei Zhu ◽  
Juanli Cheng ◽  
Biao Luo ◽  
Saixiang Feng ◽  
Jinshui Lin ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acid Biosynthesis

Soybean TILLING-by-Sequencing+ reveals the role of novel GmSACPD members in unsaturated fatty acid biosynthesis while maintaining healthy nodules

2020 ◽  
Vol 71 (22) ◽  
pp. 6969-6987
Author(s):  
Naoufal Lakhssassi ◽  
Zhou Zhou ◽  
Shiming Liu ◽  
Sarbottam Piya ◽  
Mallory A Cullen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Stearic Acid ◽  
Unsaturated Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acyl Carrier Protein ◽  
Nodule Development ◽  
Acid Biosynthesis ◽  
High Stearic Acid ◽  
Tilling By Sequencing

Abstract Developing soybean lines with high levels of stearic acid is a primary goal of the soybean industry. Most high-stearic-acid soybeans carry different GmSACPD-C mutated alleles. However, due to the dual role of GmSACPD-C in seeds and nodule development, all derived deleterious GmSACPD-C mutant alleles are of extremely poor agronomic value because of defective nodulation. The soybean stearoyl-acyl carrier protein desaturase (GmSACPD) gene family is composed of five members. Comparative genomics analysis indicated that SACPD genes were duplicated and derived from a common ancestor that is still present in chlorophytic algae. Synteny analysis showed the presence of segment duplications between GmSACPD-A/GmSACPD-B, and GmSACPD-C/GmSACPD-D. GmSACPD-E was not contained in any duplicated segment and may be the result of tandem duplication. We developed a TILLING by Target Capture Sequencing (Tilling-by-Sequencing+) technology, a versatile extension of the conventional TILLING by sequencing, and successfully identified 12, 14, and 18 ethyl methanesulfonate mutants at the GmSACPD-A, GmSACPD-B, and GmSACPD-D genes, respectively. Functional analysis of all identified mutants revealed an unprecedented role of GmSACPD-A, GmSACPD-B, and GmSACPD-D in unsaturated fatty acid biosynthesis without affecting nodule development and structure. This discovery will positively impact the development of high-stearic-acid lines to enhance soybean nutritional value without potential developmental tradeoffs.

scholarly journals SMRT Sequencing of a Full-length Transcriptome Reveals Transcript Variants Involved in C18 Unsaturated Fatty Acid Biosynthesis and Metabolism Pathways at Chilling Temperature in Pennisetum giganteum

2019 ◽  
Author(s):  
Qingyuan Li ◽  
Conglin Xiang ◽  
Lin Xu ◽  
Jinghua Cui ◽  
Shao Fu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Cold Stress ◽  
Unsaturated Fatty Acid ◽  
Stress Responses ◽  
Fatty Acid Biosynthesis ◽  
Full Length ◽  
Chilling Temperature ◽  
Acid Biosynthesis ◽  
Fast Growing ◽  
Transcript Variants

Abstract Background Pennisetum giganteum, an abundant, fast-growing perennial C4 grass that belongs to the genus Pennisetum, family Poaceae, has been developed as a source of biomass for mushroom cultivation and production, as a source of forage for cattle and sheep, and as a tool to remedy soil erosion. However, having a chilling-sensitive nature, P. giganteum seedlings need to be protected while overwintering in most temperate climate regions. Results To elucidate the cold stress responses of P. giganteum, we carried out comprehensive full-length transcriptomes from leaf and root tissues under room temperature (RT) and chilling temperature (CT) using PacBio Iso-Seq long reads. We identified 196,124 and 140,766 full-length consensus transcripts in the RT and CT samples, respectively. We then systematically performed functional annotation, transcription factor identification, long non-coding RNAs (lncRNAs) prediction, and simple sequence repeat (SSR) analysis of those full-length transcriptomes. Moreover, isoform analysis revealed that alternative splicing events may be induced by cold stress in P. giganteum, and transcript variants may be involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in P. giganteum. Conclusions We provide the first comprehensive full-length transcriptomic resource for the abundant and fast-growing perennial grass Pennisetum giganteum. Our results provide a useful genomic resource for exploring the biological pathways involved in the cold stress responses of P. giganteum.

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