scholarly journals Dimethyl Fumarate Inhibits the Nuclear Factor κB Pathway in Breast Cancer Cells by Covalent Modification of p65 Protein

2015 ◽  
Vol 291 (7) ◽  
pp. 3639-3647 ◽  
Author(s):  
Irida Kastrati ◽  
Marton I. Siklos ◽  
Esther L. Calderon-Gierszal ◽  
Lamiaa El-Shennawy ◽  
Gergana Georgieva ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Dimethyl Fumarate ◽  
Nuclear Factor Κb ◽  
Covalent Modification ◽  
Nuclear Factor

Curcumin Suppresses the Paclitaxel-Induced Nuclear Factor-κB Pathway in Breast Cancer Cells and Inhibits Lung Metastasis of Human Breast Cancer in Nude Mice

2005 ◽  
Vol 11 (20) ◽  
pp. 7490-7498 ◽  
Author(s):  
Bharat B. Aggarwal ◽  
Shishir Shishodia ◽  
Yasunari Takada ◽  
Sanjeev Banerjee ◽  
Robert A. Newman ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Lung Metastasis ◽  
Nude Mice ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Human Breast

Activation of Nuclear Factor κB by Polyamines in Breast Cancer Cells†

Biochemistry ◽  
1999 ◽  
Vol 38 (45) ◽  
pp. 14763-14774 ◽  
Author(s):  
Neha Shah ◽  
Thresia Thomas ◽  
Akira Shirahata ◽  
Leonard H. Sigal ◽  
T. J. Thomas
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Nuclear Factor Κb ◽  
Nuclear Factor

scholarly journals Suppression of Nuclear Factor-κB by Glucocorticoid Receptor Blocks Estrogen-Induced Apoptosis in Estrogen-Deprived Breast Cancer Cells

2019 ◽  
Vol 18 (10) ◽  
pp. 1684-1695 ◽  
Author(s):  
Ping Fan ◽  
Doris R. Siwak ◽  
Balkees Abderrahman ◽  
Fadeke A. Agboke ◽  
Smitha Yerrum ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Glucocorticoid Receptor ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Induced Apoptosis

scholarly journals LPS Induces GM-CSF Production by Breast Cancer MDA-MB-231 Cells via Long-Chain Acyl-CoA Synthetase 1

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4709
Author(s):  
Fatema Al-Rashed ◽  
Reeby Thomas ◽  
Areej Al-Roub ◽  
Fahd Al-Mulla ◽  
Rasheed Ahmad
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
P38 Mapk ◽  
Breast Cancer Cells ◽  
Nuclear Factor Κb ◽  
Positive Control ◽  
Nuclear Factor ◽  
Necrosis Factor Alpha ◽  
Gm Csf ◽  
Csf Production

Granulocyte–macrophage colony-stimulating factor (GM-CSF) is a monomeric glycoprotein that has been implicated in the tumor growth and progression of different types of cancer. GM-CSF is produced by various non-immune cells including MDA-MB-231 in response to various stimuli. However, the role of lipopolysaccharide (LPS) in the regulation of GM-CSF in MDA-MB-231 breast cancer cells so far remains unclear. Herein, we asked whether LPS could induce GM-CSF production in MDA-MB-231 cells, and if so, which signaling pathway was involved. MDA-MB-231 cells were treated with LPS or tumor necrosis factor alpha (TNF-α; positive control), and GM-CSF expression levels were determined by qRT-PCR, ELISA, and confocal microscopy. Phosphorylation of the mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-kB) signaling proteins were evaluated by flow cytometry. Our results show that LPS induces GM-CSF expression at both mRNA and protein levels in MDA-MBA-231 cells. Inhibition of acyl-CoA synthetase 1 (ACSL1) activity in the cells with triacsin C significantly reduces the secretion of GM-CSF. Furthermore, the inhibition of ACSL1 activity significantly blocks the LPS-mediated phosphorylation of p38 MAPK, MEK1/2, extracellular signal-regulated kinase (ERK)1/2, c-Jun NH2-terminal kinase (JNK), and nuclear factor-κB (NF-kB) in the cells. These findings provide the first evidence that LPS induces ACSL1-dependent GM-CSF gene expression in MDA-MB-231 breast cancer cells, which requires the activation of p38 MAPK, MEK1/2, ERK1/2, JNK, and NF-kB.

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