scholarly journals Identification of Ribosome-binding Protein p34 as an Intracellular Protein That Binds Acidic Fibroblast Growth Factor

2002 ◽  
Vol 277 (26) ◽  
pp. 23864-23871 ◽  
Author(s):  
Camilla Skiple Skjerpen ◽  
Jørgen Wesche ◽  
Sjur Olsnes
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Binding Protein ◽  
Fibroblast Growth ◽  
Intracellular Protein ◽  
Acidic Fibroblast Growth Factor ◽  
Ribosome Binding

scholarly journals Cloning of an intracellular protein that binds selectively to mitogenic acidic fibroblast growth factor

1998 ◽  
Vol 336 (1) ◽  
pp. 213-222 ◽  
Author(s):  
Elona KOLPAKOVA ◽  
Antoni WIĘDŁOCHA ◽  
Harald STENMARK ◽  
Olav KLINGENBERG ◽  
Pål Ø. FALNES ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
High Efficiency ◽  
Signal Sequence ◽  
Binding Protein ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor ◽  
Reading Frame ◽  
Intracellular Binding

In addition to its extracellular action, there is evidence that acidic fibroblast growth factor (aFGF) acts inside cells. To identify intracellular proteins interacting with aFGF, we screened a HeLa cell library in the yeast two-hybrid system using pLex-aFGF as a bait. A clone binding to aFGF, but not to the non-mitogenic mutant aFGF-K132E, was isolated and characterized. The insert contained an open reading frame corresponding to a novel protein of 42 kDa. The protein, termed aFGF intracellular binding protein (FIBP), is mainly hydrophilic and does not contain an N-terminal signal sequence. In vitro-translated FIBP bound specifically to a fusion protein of maltose-binding protein and aFGF. FIBP became post-translationally associated with microsomes added to the cell-free protein synthesizing system, and the membrane-associated protein bound aFGF with high efficiency. Immunoblots and fluorescence microscopy demonstrated that the protein is present in nuclei and, to a lesser extent, associated with mitochondria and other cytoplasmic membranes. The possibility is discussed that FIBP may be involved in the mitogenic action of aFGF. The nucleotide sequences described in this paper have been submitted to GeneBank database under accession numbers AF010187 (human FIBP) and AF010188 (simian FIBP).

scholarly journals Organization, chromosomal localization and promoter analysis of the gene encoding human acidic fibroblast growth factor intracellular binding protein

2000 ◽  
Vol 352 (3) ◽  
pp. 629-635 ◽  
Author(s):  
Elona KOLPAKOVA ◽  
Eirik FRENGEN ◽  
Trond STOKKE ◽  
Sjur OLSNES
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Cpg Island ◽  
Binding Protein ◽  
Amino Acid Sequences ◽  
Luciferase Reporter ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor ◽  
Reporter System ◽  
Intracellular Binding

Acidic fibroblast growth factor (aFGF) intracellular binding protein (FIBP) is a protein found mainly in the nucleus that might be involved in the intracellular function of aFGF. Here we present a comparative analysis of the deduced amino acid sequences of human, murine and Drosophila FIBP analogues and demonstrate that FIBP is an evolutionarily conserved protein. The human gene spans more than 5kb, comprising ten exons and nine introns, and maps to chromosome 11q13.1. Two slightly different splice variants found in different tissues were isolated and characterized. Sequence analysis of the region surrounding the translation start revealed a CpG island, a classical feature of widely expressed genes. Functional studies of the promoter region with a luciferase reporter system suggested a strong transcriptional activity residing within 600bp of the 5´ flanking region.

scholarly journals Mitogen-induced expression of the fibroblast growth factor-binding protein is transcriptionally repressed through a non-canonical E-box element.

2000 ◽  
Vol 275 (50) ◽  
pp. 39801
Author(s):  
Violaine K. Harris ◽  
Christine M. Coticchia ◽  
Heinz-Joachim List ◽  
Anton Wellstein ◽  
Anna Tate Riegel
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Binding Protein ◽  
Fibroblast Growth ◽  
Induced Expression ◽  
Factor Binding ◽  
E Box
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