scholarly journals Vibrio choleraeO395 Outer Membrane Vesicles Modulate Intestinal Epithelial Cells in a NOD1 Protein-dependent Manner and Induce Dendritic Cell-mediated Th2/Th17 Cell Responses

2012 ◽  
Vol 288 (6) ◽  
pp. 4299-4309 ◽  
Author(s):  
Debashree Chatterjee ◽  
Keya Chaudhuri
Keyword(s):  
Dendritic Cell ◽  
Epithelial Cells ◽  
Outer Membrane ◽  
Th17 Cell ◽  
Intestinal Epithelial Cells ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Dependent Manner ◽  
Intestinal Epithelial ◽  
Cell Responses

scholarly journals Strong Decrease in Invasive Ability and Outer Membrane Vesicle Release in Crohn's Disease-Associated Adherent-Invasive Escherichia coli Strain LF82 with the yfgL Gene Deleted

2005 ◽  
Vol 187 (7) ◽  
pp. 2286-2296 ◽  
Author(s):  
Nathalie Rolhion ◽  
Nicolas Barnich ◽  
Laurent Claret ◽  
Arlette Darfeuille-Michaud
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Outer Membrane ◽  
Culture Supernatant ◽  
Intestinal Epithelial Cells ◽  
Outer Membrane Proteins ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Intestinal Epithelial ◽  
Isogenic Mutant

ABSTRACT Adherent-invasive Escherichia coli strain LF82 recovered from a chronic lesion of a patient with Crohn's disease is able to invade cultured intestinal epithelial cells. Three mutants with impaired ability to invade epithelial cells had the Tn5phoA transposon inserted in the yfgL gene encoding the YfgL lipoprotein. A yfgL- negative isogenic mutant showed a marked decrease both in its ability to invade Intestine-407 cells and in the amount of the outer membrane proteins OmpA and OmpC in the culture supernatant, as shown by analysis of the culture supernatant protein contents by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectrometry. Transcomplementation of the LF82-ΔyfgL isogenic mutant with the cloned yfgL gene restored invasion ability and outer membrane protein release in the culture supernatant. The outer membrane proteins in the culture supernatant of strain LF82 resulted from the formation of vesicles. This was shown by Western blot analysis of periplasmic and outer membrane fraction markers typically found in outer membrane vesicles and by transmission electron microscopic analysis of ultracentrifuged cell-free LF82 supernatant pellets, indicating the presence of vesicles with a bilayered structure surrounding a central electron-dense core. Thus, deletion of the yfgL gene in strain LF82 resulted in a decreased ability to invade intestinal epithelial cells and a decreased release of outer membrane vesicles.

The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells

2015 ◽  
Vol 59 (4) ◽  
pp. 698-710 ◽  
Author(s):  
Miriam Bermudez-Brito ◽  
Neha M. Sahasrabudhe ◽  
Christiane Rösch ◽  
Henk A. Schols ◽  
Marijke M. Faas ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Dietary Fibers ◽  
Differential Effects ◽  
Cell Responses ◽  
The Impact

scholarly journals LOS oligosaccharide modification enhances dendritic cell responses to meningococcal native outer membrane vesicles expressing a non‐toxic lipid A

2013 ◽  
Vol 16 (4) ◽  
pp. 519-534 ◽  
Author(s):  
Hannah E. Jones ◽  
Alastair Copland ◽  
Hendrik Jan Hamstra ◽  
Jonathan Cohen ◽  
Jeremy Brown ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Outer Membrane ◽  
Lipid A ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Cell Responses

scholarly journals Porphyromonas gingivalis Outer Membrane Vesicles Enter Human Epithelial Cells via an Endocytic Pathway and Are Sorted to Lysosomal Compartments

2009 ◽  
Vol 77 (10) ◽  
pp. 4187-4196 ◽  
Author(s):  
Nobumichi Furuta ◽  
Kayoko Tsuda ◽  
Hiroko Omori ◽  
Tamotsu Yoshimori ◽  
Fuminobu Yoshimura ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Outer Membrane ◽  
Porphyromonas Gingivalis ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Endocytic Pathway ◽  
Host Cells ◽  
Periodontal Pathogen ◽  
Dependent Manner ◽  
Human Epithelial Cells

ABSTRACT Porphyromonas gingivalis, a periodontal pathogen, secretes outer membrane vesicles (MVs) that contain major virulence factors, including major fimbriae and proteases termed gingipains, although it is not confirmed whether MVs enter host cells. In this study, we analyzed the mechanisms involved in the interactions of P. gingivalis MVs with human epithelial cells. Our results showed that MVs swiftly adhered to HeLa and immortalized human gingival epithelial cells in a fimbria-dependent manner and then entered via a lipid raft-dependent endocytic pathway. The intracellular MVs were subsequently routed to early endosome antigen 1-associated compartments and then were sorted to lysosomal compartments within 90 min, suggesting that intracellular MVs were ultimately degraded by the cellular digestive machinery. However, P. gingivalis MVs remained there for over 24 h and significantly induced acidified compartment formation after being taken up by the cellular digestive machinery. In addition, MV entry was shown to be mediated by a novel pathway for transmission of bacterial products into host cells, a Rac1-regulated pinocytic pathway that is independent of caveolin, dynamin, and clathrin. Our findings indicate that P. gingivalis MVs efficiently enter host cells via an endocytic pathway and survive within the endocyte organelles for an extended period, which provides better understanding of the role of MVs in the etiology of periodontitis.

scholarly journals Campylobacter jejuni Outer Membrane Vesicles Play an Important Role in Bacterial Interactions with Human Intestinal Epithelial Cells

2012 ◽  
Vol 80 (12) ◽  
pp. 4089-4098 ◽  
Author(s):  
Abdi Elmi ◽  
Eleanor Watson ◽  
Pamela Sandu ◽  
Ozan Gundogdu ◽  
Dominic C. Mills ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Campylobacter Jejuni ◽  
Virulence Factors ◽  
Intestinal Epithelial Cells ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Host Cells ◽  
Intestinal Epithelial ◽  
Secretion Systems ◽  
Content Type

ABSTRACTCampylobacter jejuniis the most prevalent cause of food-borne gastroenteritis in the developed world; however, the molecular basis of pathogenesis is unclear. Secretion of virulence factors is a key mechanism by which enteric bacterial pathogens interact with host cells to enhance survival and/or damage the host. However,C. jejunilacks the virulence-associated secretion systems possessed by other enteric pathogens. Many bacterial pathogens utilize outer membrane vesicles (OMVs) for delivery of virulence factors into host cells. In the absence of prototypical virulence-associated secretion systems, OMVs could be an important alternative for the coordinated delivery ofC. jejuniproteins into host cells. Proteomic analysis ofC. jejuni11168H OMVs identified 151 proteins, including periplasmic and outer membrane-associated proteins, but also many determinants known to be important in survival and pathogenesis, including the cytolethal distending toxin (CDT).C. jejuniOMVs contained 16N-linked glycoproteins, indicating a delivery mechanism by which these periplasm-located yet immunogenic glycoproteins can interact with host cells.C. jejuniOMVs possess cytotoxic activity and induce a host immune response from T84 intestinal epithelial cells (IECs), which was not reduced by OMV pretreatment with proteinase K or polymyxin B prior to coincubation with IECs. Pretreatment of IECs with methyl-beta-cyclodextrin partially blocks OMV-induced host immune responses, indicating a role for lipid rafts in host cell plasma membranes during interactions withC. jejuniOMVs. OMVs isolated from aC. jejuni11168HcdtAmutant induced interleukin-8 (IL-8) to the same extent as did wild-type OMVs, suggesting OMV induction of IL-8 is independent of CDT.

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