scholarly journals Distal Interleukin-1β (IL-1β) Response Element of Human Matrix Metalloproteinase-13 (MMP-13) Binds Activator Protein 1 (AP-1) Transcription Factors and Regulates Gene Expression

2011 ◽  
Vol 287 (2) ◽  
pp. 1189-1197 ◽  
Author(s):  
Adam C. Schmucker ◽  
Jason B. Wright ◽  
Michael D. Cole ◽  
Constance E. Brinckerhoff
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Matrix Metalloproteinase ◽  
Interleukin 1Β ◽  
Activator Protein 1 ◽  
Response Element ◽  
Activator Protein ◽  
Matrix Metalloproteinase 13

scholarly journals Red wine metabolites modulate NF-κB, activator protein-1 and cAMP response element-binding proteins in human endothelial cells

2009 ◽  
Vol 103 (6) ◽  
pp. 807-814 ◽  
Author(s):  
Raffaella Canali ◽  
Raffaella Comitato ◽  
Roberto Ambra ◽  
Fabio Virgili
Keyword(s):  
Endothelial Cells ◽  
Transcription Factors ◽  
Binding Proteins ◽  
Red Wine ◽  
Camp Response Element Binding ◽  
Activator Protein 1 ◽  
Camp Response Element ◽  
Response Element ◽  
Activator Protein ◽  
Tnf Α

We have studied the effect of human serum, collected after red wine consumption (RWS), on TNF-α-dependent activation of transcription factors (NF-κB, activator protein-1 (AP-1) and cAMP response element-binding proteins) and on the expression of selected genes involved in cell adhesion or fibrinolysis processes in human primary endothelial cells (human umbilical vein endothelial cells (HUVEC)). Our data indicate that RWS containing RW metabolites, isolated after 40 min from an acute consume of wine (5 ml/kg body weight), induces nuclear translocation of NF-κB and AP-1 in the absence of any further stimulus. On the other hand, TNF-α treatment in the presence of RWS is associated with a delay in transcription factor activation and to a negative modulation on the expression of specific genes. Moreover, RWS stimulates c-jun binding to the tissue-type plasminogen activator cAMP responsive element consensus site modulating the expression of the specific gene downstream. These results confirm that RW metabolites affect the activity of different transcription factors playing an important preconditioning role in the modulation of the inflammatory pathway in endothelial cells. This is the first report on the effects of a complex food matrix, on the molecular mechanisms associated with inflammatory response in HUVEC cultured in condition that reproduces the physiological environment occurring in vivo.

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