scholarly journals Simple Generation of Human Induced Pluripotent Stem Cells Using Poly-β-amino Esters As the Non-viral Gene Delivery System

2011 ◽  
Vol 286 (14) ◽  
pp. 12417-12428 ◽  
Author(s):  
Núria Montserrat ◽  
Elena Garreta ◽  
Federico González ◽  
Jordán Gutiérrez ◽  
Cristina Eguizábal ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Delivery System ◽  
Pluripotent Stem Cells ◽  
Viral Gene ◽  
Gene Delivery System ◽  
Amino Esters ◽  
Induced Pluripotent ◽  
Viral Gene Delivery

scholarly journals Growth Factor-free Chondrogenic Differentiation from Induced Pluripotent Stem Cells using Minicircle Vectors

2018 ◽  
Author(s):  
Yeri Alice Rim ◽  
Yoojun Nam ◽  
Ji Hyeon Ju
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Growth Factor ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Viral Gene ◽  
Induced Pluripotent ◽  
Viral Gene Delivery

The human degenerative cartilage has low regenerative potential. Chondrocyte transplantation offers a promising strategy for cartilage treatment and regeneration. Currently chondrogenesis using human pluripotent stem cells are accomplished using human recombinant growth factors. Here, we differentiated human induced pluripotent stem cells (hiPSCs) into chondrocytes and cartilage pellet using minicircle vectors. Minicircles are used as a non-viral gene delivery system for gene therapy in various diseases. Non-viral gene delivery can produce growth factors without integrating into the host genome. Minicircle vectors containing bone morphogenetic protein 2 (BMP2) and transforming growth factor, beta 3 (TGFβ3) were successfully generated and delivered to hiPSC-derived outgrowth (OG) cells. Cell pellets generated using minicircle-transfected OG cells successfully differentiated into chondrogenic lineage. Chondrogenic pellets transfected with growth factor-encoding minicircles effectively recovered osteochondral defect in rat models. Taken together, this work shows the potential application of minicircles in cartilage regeneration using hiPSCs.

An insight into non-integrative gene delivery approaches to generate transgene-free induced pluripotent stem cells

Gene ◽  
2019 ◽  
Vol 686 ◽  
pp. 146-159 ◽  
Author(s):  
Krishna Kumar Haridhasapavalan ◽  
Manash P. Borgohain ◽  
Chandrima Dey ◽  
Bitan Saha ◽  
Gloria Narayan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
Insight Into

scholarly journals Delivery of Condensed DNA by Liposomal Non-viral Gene Delivery System into Nucleus of Dendritic Cells

2006 ◽  
Vol 29 (6) ◽  
pp. 1290-1293 ◽  
Author(s):  
Takashi Nakamura ◽  
Rumiko Moriguchi ◽  
Kentaro Kogure ◽  
Arisa Minoura ◽  
Tomoya Masuda ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Gene Delivery ◽  
Delivery System ◽  
Viral Gene ◽  
Gene Delivery System ◽  
Viral Gene Delivery ◽  
Condensed Dna
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