scholarly journals Regulation of the Matriptase-Prostasin Cell Surface Proteolytic Cascade by Hepatocyte Growth Factor Activator Inhibitor-1 during Epidermal Differentiation

2010 ◽  
Vol 285 (41) ◽  
pp. 31755-31762 ◽  
Author(s):  
Ya-Wen Chen ◽  
Jehng-Kang Wang ◽  
Feng-Pai Chou ◽  
Chiu-Yuan Chen ◽  
Ellen A. Rorke ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Surface ◽  
Hepatocyte Growth Factor ◽  
Epidermal Differentiation ◽  
Proteolytic Cascade ◽  
Hepatocyte Growth Factor Activator ◽  
Hepatocyte Growth ◽  
Activator Inhibitor

Hepatocyte growth factor activator inhibitor type 2 (HAI-2) modulates hepcidin expression by inhibiting the cell surface protease matriptase-2

2013 ◽  
Vol 450 (3) ◽  
pp. 583-593 ◽  
Author(s):  
Eva Maurer ◽  
Michael Gütschow ◽  
Marit Stirnberg
Keyword(s):  
Growth Factor ◽  
Cell Surface ◽  
Hepatocyte Growth Factor ◽  
Conditioned Medium ◽  
Clinical Importance ◽  
Hepcidin Expression ◽  
Hepatocyte Growth Factor Activator ◽  
Hepatocyte Growth ◽  
Cell Surface Protease ◽  
Activator Inhibitor

Matriptase-2, a recently identified cell surface protease, is the key enzyme of iron homoeostasis modulating the expression of the liver peptide hormone hepcidin. HAI (hepatocyte growth factor activator inhibitor) types 1 and 2 (HAI-1 and HAI-2 respectively) have been shown to inhibit the close homologue, i.e. matriptase. By co-expressing matriptase-2 and the inhibitor HAI-2 we have identified HAI-2 displaying high inhibitory potential against matriptase-2 at the cell surface as well as in conditioned medium. Accordingly, complex formation between matriptase-2 and HAI-2 was demonstrated by isolation of the complex via immobilizing either HAI-2 or matriptase-2 from lysates and conditioned medium of co-expressing cells. Furthermore, HAI-2 indirectly influences the expression of the hepcidin-encoding gene HAMP. The inhibitor abrogates the matriptase-2-mediated suppression of HAMP expression, presumably by inhibiting the supposed potential of matriptase-2 to cleave membrane-bound HJV (haemojuvelin). Taken together, the results of the present study have characterized HAI-2 as an inhibitor of matriptase-2 that modulates the synthesis of hepcidin and provides new insights into the regulatory mechanism of iron homoeostasis, with clinical importance for a treatment of iron overload diseases.

Expression of hepatocyte growth factor activator inhibitor type 1 in endothelial cells

Human Cell ◽  
2006 ◽  
Vol 19 (3) ◽  
pp. 91-97 ◽  
Author(s):  
Yutaka Akiyama ◽  
Miyuki Nagai ◽  
Wataru Komaki ◽  
Kousuke Marutsuka ◽  
Yujiro Asada ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Hepatocyte Growth Factor Activator ◽  
Inhibitor Type ◽  
Activator Inhibitor Type ◽  
Hepatocyte Growth ◽  
Activator Inhibitor
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