scholarly journals Mutational Analysis of Duck δ2 Crystallin and the Structure of an Inactive Mutant with Bound Substrate Provide Insight into the Enzymatic Mechanism of Argininosuccinate Lyase

2001 ◽  
Vol 277 (6) ◽  
pp. 4166-4175 ◽  
Author(s):  
Liliana M. Sampaleanu ◽  
Bomina Yu ◽  
P. Lynne Howell
Keyword(s):  
Mutational Analysis ◽  
Argininosuccinate Lyase ◽  
Enzymatic Mechanism ◽  
Insight Into

Mutational Analysis Provides Molecular Insight into the Carbohydrate-Binding Region of Calreticulin:  Pivotal Roles of Tyrosine-109 and Aspartate-135 in Carbohydrate Recognition†

Biochemistry ◽  
2004 ◽  
Vol 43 (1) ◽  
pp. 97-106 ◽  
Author(s):  
Mili Kapoor ◽  
Lars Ellgaard ◽  
Jayashree Gopalakrishnapai ◽  
Christiane Schirra ◽  
Emiliano Gemma ◽  
...  
Keyword(s):  
Mutational Analysis ◽  
Carbohydrate Binding ◽  
Binding Region ◽  
Carbohydrate Recognition ◽  
Insight Into

scholarly journals Oleate Hydratase in Lactobacillus delbrueckii subsp. bulgaricus LBP UFSC 2230 Catalyzes the Reversible Conversion between Linoleic Acid and Ricinoleic Acid

2021 ◽  
Author(s):  
Gabriela Christina Kuhl ◽  
Ricardo Ruiz Mazzon ◽  
Brenda Lee Simas Porto ◽  
Tâmela Zamboni Madaloz ◽  
Guilherme Razzera ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Stress Tolerance ◽  
Lactobacillus Delbrueckii ◽  
Content Type ◽  
E Coli ◽  
Enzymatic Mechanism ◽  
The Impact ◽  
Insight Into ◽  
Oleate Hydratase ◽  
Lactobacillus Delbrueckii Subsp

This study provides insight into the enzymatic mechanism of CLA synthesis in L. delbrueckii subsp. bulgaricus and broadens our understanding of the bioconversion of LA and RA by OleH. The impact of OleH on the production of the c 9, t 11 CLA isomer and stress tolerance by E. coli has been assisted.

scholarly journals pFiD188, the Linear Virulence Plasmid of Rhodococcus fascians D188

2012 ◽  
Vol 25 (5) ◽  
pp. 637-647 ◽  
Author(s):  
Isolde Francis ◽  
Annick De Keyser ◽  
Philippe De Backer ◽  
Carmen Simón-Mateo ◽  
Jutta Kalkus ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Mutational Analysis ◽  
Linear Plasmid ◽  
Virulence Plasmid ◽  
Symptom Development ◽  
Linear Plasmids ◽  
Type Iv ◽  
Rhodococcus Fascians ◽  
Syntenic Regions ◽  
Insight Into

Rhodococcus fascians is currently the only phytopathogen of which the virulence genes occur on a linear plasmid. To get insight into the origin of this replicon and into the virulence strategy of this broad-spectrum phytopathogen, the sequence of the linear plasmid of strain D188, pFiD188, was determined. Analysis of the 198,917 bp revealed four syntenic regions with linear plasmids of R. erythropolis, R. jostii, and R. opacus, suggesting a common origin of these replicons. Mutational analysis of pFi_086 and pFi_102, similar to cutinases and type IV peptidases, respectively, showed that conserved region R2 was involved in plasmid dispersal and pointed toward a novel function for actinobacterial cutinases in conjugation. Additionally, pFiD188 had three regions that were unique for R. fascians. Functional analysis of the stk and nrp loci of regions U2 and U3, respectively, indicated that their role in symptom development was limited compared with that of the previously identified fas, att, and hyp virulence loci situated in region U1. Thus, pFiD188 is a typical rhodococcal linear plasmid with a composite structure that encodes core functions involved in plasmid maintenance and accessory functions, some possibly acquired through horizontal gene transfer, implicated in virulence and the interaction with the host.

Mutational analysis of the CG recognizing DNA methyltransferase SssI: Insight into enzyme–DNA interactions

2009 ◽  
Vol 1794 (11) ◽  
pp. 1654-1662 ◽  
Author(s):  
Maria V. Darii ◽  
Natalia A. Cherepanova ◽  
Oksana M. Subach ◽  
Olga V. Kirsanova ◽  
Tamás Raskó ◽  
...  
Keyword(s):  
Dna Methyltransferase ◽  
Mutational Analysis ◽  
Dna Interactions ◽  
Insight Into

Mutational analysis of ProTx-I and the novel venom peptide Pe1b provide insight into residues responsible for selective inhibition of the analgesic drug target NaV1.7

2020 ◽  
Vol 181 ◽  
pp. 114080 ◽  
Author(s):  
Darshani B. Rupasinghe ◽  
Volker Herzig ◽  
Irina Vetter ◽  
Zoltan Dekan ◽  
John Gilchrist ◽  
...  
Keyword(s):  
Drug Target ◽  
Mutational Analysis ◽  
Selective Inhibition ◽  
The Novel ◽  
Analgesic Drug ◽  
Venom Peptide ◽  
Insight Into

scholarly journals Mutational Analysis of the vacA Promoter Provides Insight into Gene Transcription in Helicobacter pylori

1999 ◽  
Vol 181 (7) ◽  
pp. 2261-2266 ◽  
Author(s):  
Mark H. Forsyth ◽  
Timothy L. Cover
Keyword(s):  
Helicobacter Pylori ◽  
Gene Transcription ◽  
Functional Role ◽  
Mutational Analysis ◽  
Consensus Sequence ◽  
H Pylori ◽  
Insight Into

ABSTRACT Analysis of 12 Helicobacter pylori promoters indicates the existence of a consensus −10 hexamer (TAtaaT) but little conservation of −35 sequences. In this study, mutations in either the H. pylori vacA −10 region or the −35 region resulted in decreased vacA transcription and suggested that an extended −10 motif is utilized. Thus, despite the lack of a −35 consensus sequence for H. pylori promoters, the −35 region plays a functional role in vacA transcription.

scholarly journals Structural, Functional, and Mutational Analysis of the NblA Protein Provides Insight into Possible Modes of Interaction with the Phycobilisome

2008 ◽  
Vol 283 (44) ◽  
pp. 30330-30340 ◽  
Author(s):  
Monica Dines ◽  
Eleonora Sendersky ◽  
Liron David ◽  
Rakefet Schwarz ◽  
Noam Adir
Keyword(s):  
Mutational Analysis ◽  
Modes Of Interaction ◽  
Insight Into

A Duck δ1 Crystallin Double Loop Mutant Provides Insight into Residues Important for Argininosuccinate Lyase Activity†,‡

Biochemistry ◽  
2004 ◽  
Vol 43 (37) ◽  
pp. 11672-11682 ◽  
Author(s):  
May Tsai ◽  
Liliana M. Sampaleanu ◽  
Caroline Greene ◽  
Louise Creagh ◽  
Charles Haynes ◽  
...  
Keyword(s):  
Double Loop ◽  
Argininosuccinate Lyase ◽  
Lyase Activity ◽  
Insight Into
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