scholarly journals Chromatin Remodeling by the Thyroid Hormone Receptor in Regulation of the Thyroid-stimulating Hormone α-Subunit Promoter

2001 ◽  
Vol 276 (36) ◽  
pp. 34227-34234 ◽  
Author(s):  
Trevor N. Collingwood ◽  
Fyodor D. Urnov ◽  
V. Krishna K. Chatterjee ◽  
Alan P. Wolffe
Keyword(s):  
Thyroid Hormone ◽  
Chromatin Remodeling ◽  
Hormone Receptor ◽  
Thyroid Hormone Receptor ◽  
Thyroid Stimulating Hormone ◽  
Α Subunit ◽  
Stimulating Hormone

scholarly journals Low free thyroxine and normal thyroid-stimulating hormone in infants and children: possible causes and diagnostic work-up

2021 ◽  
Author(s):  
Peter Lauffer ◽  
A. S. Paul van Trotsenburg ◽  
Nitash Zwaveling-Soonawala
Keyword(s):  
Thyroid Hormone ◽  
Hormone Receptor ◽  
Thyroid Hormone Receptor ◽  
Thyroid Stimulating Hormone ◽  
Free Thyroxine ◽  
Infants And Children ◽  
Clinical Algorithm ◽  
Hpt Axis ◽  
In Infants And Children ◽  
Stimulating Hormone

AbstractScreening for hypo- or hyperthyroidism in adults is generally done by measuring the serum thyrotropin (thyroid-stimulating hormone, TSH) concentration. This is an efficient approach in case of suspected acquired thyroid disease. However, in infants and children, congenital hypothalamus-pituitary-thyroid (HPT) axis disorders also need to be considered, including primary and central congenital hypothyroidism, and even rarer thyroid hormone receptor and transporter defects. In primary congenital hypothyroidism, TSH will be elevated, but in the other congenital HPT axis disorders, TSH is usually within the normal range. Free thyroxine (FT4) assessment is essential for the diagnosis in these conditions.Conclusion: Here we discuss a number of rare congenital HPT axis disorders in which TSH is normal, but FT4 is low, and provide a clinical algorithm to distinguish between these disorders. What is Known:• A single thyroid-stimulating hormone (TSH) measurement is an appropriate screening method for primary hypothyroidism.• For central hypothyroidism and rare thyroid hormone receptor and transporter defects a free thyroxine (FT4) measurement is essential for the diagnosis because TSH is usually normal. What is New:• Here we present a new problem-oriented clinical algorithm including a diagnostic flow-chart for low FT4 and normal TSH in infants and children.

scholarly journals Thyroid-hormone-dependent negative regulation of thyrotropin beta gene by thyroid hormone receptors: study with a new experimental system using CV1 cells

2004 ◽  
Vol 378 (2) ◽  
pp. 549-557 ◽  
Author(s):  
Keiko NAKANO ◽  
Akio MATSUSHITA ◽  
Shigekazu SASAKI ◽  
Hiroko MISAWA ◽  
Kozo NISHIYAMA ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Hormone Receptor ◽  
Hormone Receptors ◽  
Thyroid Hormone Receptor ◽  
Experimental System ◽  
Thyroid Stimulating Hormone ◽  
Negative Regulation ◽  
Dominant Negative ◽  
Negative Effects ◽  
Receptor Isoforms

The molecular mechanism involved in the liganded thyroid hormone receptor suppression of the TSHβ (thyroid-stimulating hormone β, or thyrotropin β) gene transcription is undetermined. One of the main reasons is the limitation of useful cell lines for the experiments. We have developed an assay system using non-pituitary CV1 cells and studied the negative regulation of the TSHβ gene. In CV1 cells, the TSHβ–CAT (chloramphenicol acetyltransferase) reporter was stimulated by Pit1 and GATA2 and suppressed by T3 (3,3´,5-tri-iodothyronine)-bound thyroid hormone receptor. The suppression was dependent on the amounts of T3 and the receptor. Unliganded receptor did not stimulate TSHβ activity, suggesting that the receptor itself is not an activator. Analyses using various receptor mutants revealed that the intact DNA-binding domain is crucial to the TSHβ gene suppression. Co-activators and co-repressors are not necessarily essential, but are required for the full suppression of the TSHβ gene. Among the three receptor isoforms, β2 exhibited the strongest inhibition and its protein level was the most predominant in a thyrotroph cell line, TαT1, in Western blotting. The dominant-negative effects of various receptor mutants measured on the TSHβ–CAT reporter were not simple mirror images of those in the positive regulation under physiological T3 concentration.

Recombinant thyroid antigens: preparation and application in the diagnosis and treatment of autoimmune diseases

2021 ◽  
pp. 48-53
Author(s):  
Alexander Vladimirovich Zubkov
Keyword(s):  
Autoimmune Diseases ◽  
Hormone Receptor ◽  
Extracellular Domain ◽  
Thyroid Stimulating Hormone ◽  
Human Thyroid ◽  
Antigenic Structure ◽  
Thyroid Peroxidase ◽  
Α Subunit ◽  
Thyroid Autoantigens ◽  
Stimulating Hormone

New opportunities are opening up in the study of the relationship between the antigenic structure of thyroid autoantigens and autoantibodies in the pathogenesis of such autoimmune diseases as Graves’ disease (GD) and autoimmune thyroiditis (AIT) using synthesized recombinant proteins of the thyroid-stimulating hormone receptor (TSHR) and human thyroid peroxidase (TPO). In the present work, the results of cloning of the fragments of the TPO extracellular domain and fragments of the RNA sequence of the α-subunit of TSHR, which do not contain nucleotide substitutions, are demonstrated. Recombinant vectors for the expression of proteins of the α-subunit of thyroid-stimulating hormone receptor and fragments of the TPO extracellular domain have been obtained.

Thyroid-stimulating hormone receptor and thyroid hormone receptors are involved in human endometrial physiology

2011 ◽  
Vol 95 (1) ◽  
pp. 230-237.e2 ◽  
Author(s):  
Lusine Aghajanova ◽  
Anneli Stavreus-Evers ◽  
Maria Lindeberg ◽  
Britt-Marie Landgren ◽  
Lottie Skjöldebrand Sparre ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Hormone Receptor ◽  
Hormone Receptors ◽  
Thyroid Stimulating Hormone ◽  
Thyroid Hormone Receptors ◽  
Stimulating Hormone
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