scholarly journals The Photocycle of a Flavin-binding Domain of the Blue Light Photoreceptor Phototropin

2001 ◽  
Vol 276 (39) ◽  
pp. 36493-36500 ◽  
Author(s):  
Trevor E. Swartz ◽  
Stephanie B. Corchnoy ◽  
John M. Christie ◽  
James W. Lewis ◽  
Istvan Szundi ◽  
...  
Keyword(s):  
Blue Light ◽  
Binding Domain ◽  
Blue Light Photoreceptor ◽  
Flavin Binding

Identification of Six New Photoactive Yellow ProteinsDiversity and StructureFunction Relationships in a Bacterial Blue Light Photoreceptor

2008 ◽  
Vol 84 (4) ◽  
pp. 956-969 ◽  
Author(s):  
Masato Kumauchi ◽  
Miwa T. Hara ◽  
Page Stalcup ◽  
Aihua Xie ◽  
Wouter D. Hoff
Keyword(s):  
Blue Light ◽  
Blue Light Photoreceptor

scholarly journals Blakeslea trispora Photoreceptors: Identification and Functional Analysis

2020 ◽  
Vol 86 (8) ◽  
Author(s):  
Wei Luo ◽  
Chao Xue ◽  
Yuzheng Zhao ◽  
Huili Zhang ◽  
Zhiming Rao ◽  
...  
Keyword(s):  
Large Scale ◽  
Binding Domain ◽  
Site Directed Mutagenesis ◽  
Flavin Mononucleotide ◽  
Blakeslea Trispora ◽  
Scale Production ◽  
Bioinformatics Analyses ◽  
Content Type ◽  
Flavin Binding

ABSTRACT Blakeslea trispora is an industrial fungal species used for large-scale production of carotenoids. However, B. trispora light-regulated physiological processes, such as carotenoid biosynthesis and phototropism, are not fully understood. In this study, we isolated and characterized three photoreceptor genes, btwc-1a, btwc-1b, and btwc-1c, in B. trispora. Bioinformatics analyses of these genes and their protein sequences revealed that the functional domains (PAS/LOV [Per-ARNT-Sim/light-oxygen-voltage] domain and zinc finger structure) of the proteins have significant homology to those of other fungal blue-light regulator proteins expressed by Mucor circinelloides and Neurospora crassa. The photoreceptor proteins were synthesized by heterologous expression in Escherichia coli. The chromogenic groups consisting of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) were detected to accompany BTWC-1 proteins by using high-performance liquid chromatography (HPLC) and fluorescence spectrometry, demonstrating that the proteins may be photosensitive. The absorbance changes of the purified BTWC-1 proteins seen under dark and light conditions indicated that they were light responsive and underwent a characteristic photocycle by light induction. Site-directed mutagenesis of the cysteine residual (Cys) in BTWC-1 did not affect the normal expression of the protein in E. coli but did lead to the loss of photocycle response, indicating that Cys represents a flavin-binding domain for photon detection. We then analyzed the functions of BTWC-1 proteins by complementing btwc-1a, btwc-1b, and btwc-1c into the counterpart knockout strains of M. circinelloides for each mcwc-1 gene. Transformation of the btwc-1a complement into mcwc-1a knockout strains restored the positive phototropism, while the addition of btwc-1c complement remedied the deficiency of carotene biosynthesis in the mcwc-1c knockout strains under conditions of illumination. These results indicate that btwc-1a and btwc-1c are involved in phototropism and light-inducible carotenogenesis. Thus, btwc-1 genes share a conserved flavin-binding domain and act as photoreceptors for control of different light transduction pathways in B. trispora. IMPORTANCE Studies have confirmed that light-regulated carotenogenesis is prevalent in filamentous fungi, especially in mucorales. However, few investigations have been done to understand photoinduced synthesis of carotenoids and related mechanisms in B. trispora, a well-known industrial microbial strains. In the present study, three photoreceptor genes in B. trispora were cloned, expressed, and characterized by bioinformatics and photoreception analyses, and then in vivo functional analyses of these genes were constructed in M. circinelloides. The results of this study will lead to a better understanding of photoreception and light-regulated carotenoid synthesis and other physiological responses in B. trispora.

Is the photoactive yellow protein a UV-B/blue light photoreceptor?

2011 ◽  
Vol 10 (4) ◽  
pp. 464 ◽  
Author(s):  
Elizabeth C. Carroll ◽  
Marijke Hospes ◽  
Carmen Valladares ◽  
Klaas J. Hellingwerf ◽  
Delmar S. Larsen
Keyword(s):  
Blue Light ◽  
Protein A ◽  
Photoactive Yellow Protein ◽  
Blue Light Photoreceptor

scholarly journals Manipulation of the Blue Light Photoreceptor Cryptochrome 2 in Tomato Affects Vegetative Development, Flowering Time, and Fruit Antioxidant Content

2004 ◽  
Vol 137 (1) ◽  
pp. 199-208 ◽  
Author(s):  
Leonardo Giliberto ◽  
Gaetano Perrotta ◽  
Patrizia Pallara ◽  
James L. Weller ◽  
Paul D. Fraser ◽  
...  
Keyword(s):  
Flowering Time ◽  
Blue Light ◽  
Vegetative Development ◽  
Blue Light Photoreceptor ◽  
Antioxidant Content ◽  
Cryptochrome 2

Crystal Structure ofParacoccus denitrificansElectron Transfer Flavoprotein:  Structural and Electrostatic Analysis of a Conserved Flavin Binding Domain†,‡

Biochemistry ◽  
1999 ◽  
Vol 38 (7) ◽  
pp. 1977-1989 ◽  
Author(s):  
David L. Roberts ◽  
Denise Salazar ◽  
John P. Fulmer ◽  
Frank E. Frerman ◽  
Jung-Ja P. Kim
Keyword(s):  
Crystal Structure ◽  
Binding Domain ◽  
Flavin Binding

scholarly journals Single mutation in a novel bacterial LOV protein yields a singlet oxygen generator

2019 ◽  
Vol 18 (11) ◽  
pp. 2657-2660 ◽  
Author(s):  
Eleonora Consiglieri ◽  
Qianzhao Xu ◽  
Mikkel Bregnhøj ◽  
Michael Westberg ◽  
Peter R. Ogilby ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Blue Light ◽  
Single Mutation ◽  
Facultative Methylotroph ◽  
Blue Light Photoreceptor ◽  
Oxygen Generator ◽  
Singlet Oxygen Generator

The first blue light photoreceptor isolated from Methylobacterium radiotolerans, a pink-pigmented facultative methylotroph, has been turned into an efficient singlet oxygen generator by introducing a single mutation.

Riboflavin-binding sites associated with flagella of Euglena: A candidate for blue-light photoreceptor?

Planta ◽  
1991 ◽  
Vol 185 (1) ◽  
Author(s):  
A. Nebenf�hr ◽  
A. Sch�fer ◽  
P. Galland ◽  
H. Senger ◽  
R. Hertel
Keyword(s):  
Blue Light ◽  
Binding Sites ◽  
Blue Light Photoreceptor

Signaling States of a Short Blue-Light Photoreceptor Protein PpSB1-LOV Revealed from Crystal Structures and Solution NMR Spectroscopy

2016 ◽  
Vol 428 (19) ◽  
pp. 3721-3736 ◽  
Author(s):  
Katrin Röllen ◽  
Joachim Granzin ◽  
Vineet Panwalkar ◽  
Vladimir Arinkin ◽  
Raj Rani ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Crystal Structures ◽  
Blue Light ◽  
Solution Nmr ◽  
Blue Light Photoreceptor ◽  
Solution Nmr Spectroscopy
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