scholarly journals Role of Accessory Factors and Steroid Receptor Coactivator 1 in the Regulation of Phosphoenolpyruvate Carboxykinase Gene Transcription by Glucocorticoids

2000 ◽  
Vol 276 (6) ◽  
pp. 3811-3819 ◽  
Author(s):  
John M. Stafford ◽  
Mary Waltner-Law ◽  
Daryl K. Granner
Keyword(s):  
Gene Transcription ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Steroid Receptor ◽  
Steroid Receptor Coactivator ◽  
Accessory Factors ◽  
Phosphoenolpyruvate Carboxykinase Gene

Role of glucocorticoids in activation of hepatic PEPCK gene transcription during exercise

1994 ◽  
Vol 266 (4) ◽  
pp. E560-E566 ◽  
Author(s):  
J. E. Friedman
Keyword(s):  
Transgenic Mice ◽  
Gene Transcription ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Mrna Levels ◽  
Camp Response Element ◽  
Carbohydrate Diet ◽  
High Carbohydrate ◽  
Enhancer Binding Protein ◽  
Response To Exercise

The objective of these studies was to determine the molecular basis for the activation of phosphoenolpyruvate carboxykinase (PEPCK) gene transcription during prolonged submaximal exercise. Mice were fed a high-carbohydrate diet for 1 wk and exercised continuously by swimming for up to 120 min. The level of hepatic PEPCK mRNA increased progressively during exercise, reaching 510% above control, whereas transcription of the PEPCK gene increased 1,000%, before decreasing to control levels within 60 min of recovery. In transgenic mice carrying a chimeric gene consisting of the PEPCK promoter linked to a reporter gene for bovine growth hormone (bGH), PEPCK(-460)-bGH, the level of hepatic bGH mRNA increased by 490% in response to exercise, similar to the increase in the expression of the native PEPCK gene. However, in transgenic mice with a deletion of the glucocorticoid regulatory unit, PEPCK(-355)-bGH, bGH mRNA did not increase above control values. In transgenic mice with a block mutation in adenosine 3',5'-cyclic monophosphate (cAMP) regulatory regions -90/-82 and -250/-234, PEPCK cAMP response element 1 (CRE-1)/P3(1)-bGH, exercise increased bGH mRNA 260% above controls. Adrenalectomy (Adx) had no effect on PEPCK mRNA levels in nonexercised mice, whereas in adrenalectomized (Adx)-exercised mice, PEPCK mRNA increased only 80% above basal, and, in Adx mice injected with dexamethasone, PEPCK mRNA increased with exercise 570% above controls. Exercise was also associated with a large increase in transcription of the gene for the transcription factor CCAAT/enhancer-binding protein beta (C/EBP-beta) and a smaller rise in transcription of c-jun gene, both of which returned to control levels during recovery.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals C/EBP and the Control of Phosphoenolpyruvate Carboxykinase Gene Transcription in the Liver

1998 ◽  
Vol 273 (48) ◽  
pp. 31629-31632 ◽  
Author(s):  
Colleen Croniger ◽  
Patrick Leahy ◽  
Lea Reshef ◽  
Richard W. Hanson
Keyword(s):  
Gene Transcription ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Phosphoenolpyruvate Carboxykinase Gene
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