scholarly journals Isopentenyl Pyrophosphate, a Mycobacterial Non-peptidic Antigen, Triggers Delayed and Highly Sustained Signaling in Human γδ T Lymphocytes without Inducing Down-modulation of T Cell Antigen Receptor

2001 ◽  
Vol 276 (19) ◽  
pp. 15961-15967 ◽  
Author(s):  
Virginie Lafont ◽  
Janny Liautard ◽  
Magali Sablé-Teychené ◽  
Yannis Sainte-Marie ◽  
Jean Favero
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Antigen Receptor ◽  
T Cell Antigen Receptor ◽  
Isopentenyl Pyrophosphate ◽  
Cell Antigen Receptor ◽  
Cell Antigen

scholarly journals The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8- T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer.

1987 ◽  
Vol 165 (4) ◽  
pp. 1076-1094 ◽  
Author(s):  
L L Lanier ◽  
N A Federspiel ◽  
J J Ruitenberg ◽  
J H Phillips ◽  
J P Allison ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cell Lines ◽  
Peripheral Blood ◽  
Antigen Receptor ◽  
Receptor Complex ◽  
T Cell Antigen Receptor ◽  
Normal Peripheral Blood ◽  
Cell Antigen Receptor ◽  
Cell Antigen

IL-2-dependent cell lines were established from normal peripheral blood T lymphocytes that express neither CD4 nor CD8 differentiation antigens. CD3+,4-,8- cell lines from 15 different donors failed to react with WT31, an mAb directed against the T cell antigen receptor alpha/beta heterodimer. Anti-Leu-4 mAb was used to isolate the CD3/T cell antigen receptor complex from 125I-labeled CD3+,4-,8- (WT31-) T cells. Using detergent conditions that preserved the CD3/T cell antigen receptor complex, an approximately 90 kD disulfide-linked heterodimer, composed of approximately 45- and approximately 40- (or approximately 37-) kD subunits, was coimmunoprecipitated with the invariant 20-29-kD CD3 complex. Analysis of these components by nonequilibrium pH gradient electrophoresis indicated that the approximately 40-kD and approximately 37-kD subunits were similar, and quite distinct from the more basic approximately 45-kD subunit. None of these three subunits reacted with an antibody directed against a beta chain framework epitope. Heteroantiserum against a T cell receptor gamma chain peptide specifically reacted with both the approximately 37- and approximately 40-kD CD3-associated proteins, but not with the approximately 45-kD subunit. CD3+,4-,8- cells failed to transcribe substantial amounts of functional 1.3-kb beta or 1.6-kb alpha mRNA, but produced abundant 1.6-kb gamma mRNA. Southern blot analysis revealed that these CD3+,4-,8- cell lines rearranged both gamma and beta genes, and indicated that the populations were polyclonal. The expression of a CD3-associated disulfide-linked heterodimer on CD3+,4-,8- T cell lines established from normal, adult peripheral blood contrasts with prior reports describing a CD3-associated non-disulfide-linked heterodimer on CD3+/WT31- cell lines established from thymus and peripheral blood obtained from patients with immunodeficiency diseases. We propose that this discrepancy may be explained by preferential usage of the two C gamma genes in T lymphocytes.

CD8 modulation of T-cell antigen receptor–ligand interactions on living cytotoxic T lymphocytes

Nature ◽  
1995 ◽  
Vol 373 (6512) ◽  
pp. 353-356 ◽  
Author(s):  
Immanuel F. Luescher ◽  
Eric Vivier ◽  
Andréas Layer ◽  
Jérôme Mahiou ◽  
François Godeau ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Antigen Receptor ◽  
T Cell Antigen Receptor ◽  
Receptor Ligand ◽  
Cell Antigen Receptor ◽  
Cell Antigen ◽  
Ligand Interactions

Human T lymphocytes expressing T cell antigen receptor

1989 ◽  
Vol 50 (1) ◽  
pp. S117-S123 ◽  
Author(s):  
L. Moretta ◽  
E. Ciccone ◽  
M.C. Mingari ◽  
C. Bottino ◽  
S. Ferrini ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Antigen Receptor ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Cell Antigen ◽  
Human T Lymphocytes
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