scholarly journals Membrane Translocation of Novel Protein Kinase Cs Is Regulated by Phosphorylation of the C2 Domain

2000 ◽  
Vol 276 (6) ◽  
pp. 3846-3855 ◽  
Author(s):  
Antonio M. Pepio ◽  
Wayne S. Sossin
Keyword(s):  
Protein Kinase ◽  
C2 Domain ◽  
Membrane Translocation ◽  
Novel Protein

scholarly journals Physiological Role for Phosphatidic Acid in the Translocation of the Novel Protein Kinase C Apl II in Aplysia Neurons

2008 ◽  
Vol 28 (15) ◽  
pp. 4719-4733 ◽  
Author(s):  
Carole A. Farah ◽  
Ikue Nagakura ◽  
Daniel Weatherill ◽  
Xiaotang Fan ◽  
Wayne S. Sossin
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Phosphatidic Acid ◽  
Physiological Role ◽  
C2 Domain ◽  
The Novel ◽  
Physiological Conditions ◽  
Kinase C ◽  
Neuronal Membranes ◽  
Novel Protein

ABSTRACT In Aplysia californica, the serotonin-mediated translocation of protein kinase C (PKC) Apl II to neuronal membranes is important for synaptic plasticity. The orthologue of PKC Apl II, PKCε, has been reported to require phosphatidic acid (PA) in conjunction with diacylglycerol (DAG) for translocation. We find that PKC Apl II can be synergistically translocated to membranes by the combination of DAG and PA. We identify a mutation in the C1b domain (arginine 273 to histidine; PKC Apl II-R273H) that removes the effects of exogenous PA. In Aplysia neurons, the inhibition of endogenous PA production by 1-butanol inhibited the physiological translocation of PKC Apl II by serotonin in the cell body and at the synapse but not the translocation of PKC Apl II-R273H. The translocation of PKC Apl II-R273H in the absence of PA was explained by two additional effects of this mutation: (i) the mutation removed C2 domain-mediated inhibition, and (ii) the mutation decreased the concentration of DAG required for PKC Apl II translocation. We present a model in which, under physiological conditions, PA is important to activate the novel PKC Apl II both by synergizing with DAG and removing C2 domain-mediated inhibition.

Structure of the C2 domain from novel protein kinase Cϵ. A membrane binding model for Ca2+-independent C2 domains

2001 ◽  
Vol 311 (4) ◽  
pp. 837-849 ◽  
Author(s):  
Wendy F Ochoa ◽  
Josefa Garcia-Garcia ◽  
Ignacio Fita ◽  
Senena Corbalan-Garcia ◽  
Nuria Verdaguer ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Membrane Binding ◽  
C2 Domain ◽  
Binding Model ◽  
C2 Domains ◽  
Novel Protein

scholarly journals Autophosphorylation of the C2 domain inhibits translocation of the novel protein kinase C (nPKC) Apl II

2012 ◽  
Vol 123 (3) ◽  
pp. 360-372 ◽  
Author(s):  
Carole A. Farah ◽  
Amanda A. Lindeman ◽  
Vincent Siu ◽  
Micaela Das Gupta ◽  
Wayne S. Sossin
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
C2 Domain ◽  
The Novel ◽  
Kinase C ◽  
Novel Protein

scholarly journals Structural characterization of the C2 domain of novel protein kinase Cε

2001 ◽  
Vol 268 (4) ◽  
pp. 1107-1117 ◽  
Author(s):  
Josefa García-García ◽  
Juan C. Gómez-Fernández ◽  
Senena Corbalán-García
Keyword(s):  
Protein Kinase ◽  
Structural Characterization ◽  
C2 Domain ◽  
Novel Protein

scholarly journals W55aEncodes a Novel Protein Kinase That Is Involved in Multiple Stress Responses

2009 ◽  
Vol 51 (1) ◽  
pp. 58-66 ◽  
Author(s):  
Zhao-Shi Xu ◽  
Li Liu ◽  
Zhi-Yong Ni ◽  
Pei Liu ◽  
Ming Chen ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Stress Responses ◽  
Multiple Stress ◽  
Novel Protein

scholarly journals PfPKB, a Novel Protein Kinase B-like Enzyme fromPlasmodium falciparum

2004 ◽  
Vol 279 (23) ◽  
pp. 24255-24264 ◽  
Author(s):  
Amit Kumar ◽  
Ankush Vaid ◽  
Chiang Syin ◽  
Pushkar Sharma
Keyword(s):  
Protein Kinase ◽  
Protein Kinase B ◽  
Novel Protein
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