scholarly journals CD43-mediated Signals Induce DNA Binding Activity of AP-1, NF-AT, and NFκB Transcription Factors in Human T Lymphocytes

2000 ◽  
Vol 275 (40) ◽  
pp. 31460-31468 ◽  
Author(s):  
M. Angélica Santana ◽  
Gustavo Pedraza-Alva ◽  
Norma Olivares-Zavaleta ◽  
Vicente Madrid-Marina ◽  
Vaclav Horejsi ◽  
...  
Keyword(s):  
Transcription Factors ◽  
T Lymphocytes ◽  
Dna Binding ◽  
Binding Activity ◽  
Human T Lymphocytes ◽  
Dna Binding Activity

scholarly journals Direct inhibition of the DNA-binding activity of POU transcription factors Pit-1 and Brn-3 by selective binding of a phenyl-furan-benzimidazole dication

2008 ◽  
Vol 36 (10) ◽  
pp. 3341-3353 ◽  
Author(s):  
Paul Peixoto ◽  
Yang Liu ◽  
Sabine Depauw ◽  
Marie-Paule Hildebrand ◽  
David W. Boykin ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Binding Activity ◽  
Direct Inhibition ◽  
Selective Binding ◽  
Dna Binding Activity

Ozone-induced IL-8 expression and transcription factor binding in respiratory epithelial cells

1997 ◽  
Vol 272 (3) ◽  
pp. L504-L511 ◽  
Author(s):  
I. Jaspers ◽  
E. Flescher ◽  
L. C. Chen
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Epithelial Cells ◽  
Inflammatory Cells ◽  
Binding Activity ◽  
Ozone Exposure ◽  
Air Exposure ◽  
Dna Binding Activity ◽  
Nf Kappab

Ozone, one of the most reactive oxidant gases to which humans are routinely exposed, induces inflammation in the lower airways. The airway epithelium is one of the first targets that inhaled ozone will encounter, but its role in airway inflammation is not well understood. Expression of inducible genes involved in the inflammatory response, such as interleukin (IL)-8, is controlled by transcription factors. Expression of the IL-8 gene is regulated by the transcription factors nuclear factor (NF)-kappaB, NF-IL-6, and possibly activator protein-1 (AP-1). Type II-like epithelial cells (A549) were grown on a collagen-coated membrane and exposed in vitro to 0.1 ppm ozone or air. Exposure to ozone induced DNA-binding activity of NF-kappaB, NF-IL-6, and AP-1. IL-8 mRNA and IL-8 protein levels were also increased after ozone exposure. These results link ozone-induced DNA-binding activity of transcription factors and the production of IL-8 by epithelial cells thus demonstrating a potential cellular cascade resulting in the recruitment of inflammatory cells into the airway lumen.

scholarly journals Expression of the genes encoding CCAAT-enhancer binding protein isoforms in the mouse mammary gland during lactation and involution

1998 ◽  
Vol 334 (1) ◽  
pp. 205-210 ◽  
Author(s):  
Georgios SABATAKOS ◽  
Gareth E. DAVIES ◽  
Maria GROSSE ◽  
Anthony CRYER ◽  
Dipak P. RAMJI
Keyword(s):  
Transcription Factors ◽  
Mammary Gland ◽  
Dna Binding ◽  
Binding Protein ◽  
Mouse Mammary Gland ◽  
Binding Activity ◽  
Protein Isoforms ◽  
Dna Binding Activity ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein

Transcription factors belonging to the CCAAT-enhancer binding protein (C/EBP) family have been implicated in the activation of gene expression in the mammary gland during lactation. We have therefore investigated the detailed expression profile of the C/EBP family during lactation and involution of the mouse mammary gland. The expression of C/EBPβ and C/EBPδ mRNA was low during lactation, increased dramatically at the beginning of involution and remained constant thereafter. In contrast, C/EBPα mRNA expression was relatively high during the early stages of lactation, declined to low levels during the late stages of lactation and at the start of involution, and increased again during involution. Electrophoretic mobility-shift assays showed a close correlation between the expression of the C/EBP genes and the functional C/EBP DNA-binding activity and, additionally, demonstrated the participation of heterodimers, formed from among the three proteins, in DNA–protein interactions. The DNA-binding activity of the activator protein 1 (AP1) family of transcription factors was also induced during involution. These results therefore point to potentially important regulatory roles for both the C/EBP and the AP1 family during lactation and involution of the mammary gland.

Examination of DNA-binding activity of neuronal transcription factors by electrophoretical mobility shift assay

1998 ◽  
Vol 2 (4) ◽  
pp. 243-249 ◽  
Author(s):  
Koichiro Kako ◽  
Hisanori Wakamatsu ◽  
Toshiyuki Hamada ◽  
Marek Banasik ◽  
Keiko Ohata ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Binding Activity ◽  
Mobility Shift ◽  
Dna Binding Activity ◽  
Mobility Shift Assay

scholarly journals Interleukin-7 Upregulates the Interleukin-2–Gene Expression in Activated Human T Lymphocytes at the Transcriptional Level by Enhancing the DNA Binding Activities of Both Nuclear Factor of Activated T Cells and Activator Protein-1

Blood ◽  
1997 ◽  
Vol 90 (7) ◽  
pp. 2690-2700 ◽  
Author(s):  
Sonja I. Gringhuis ◽  
Lou F.M.H. de Leij ◽  
Emmy W. Verschuren ◽  
Peter Borger ◽  
Edo Vellenga
Keyword(s):  
T Lymphocytes ◽  
Dna Binding ◽  
Binding Activity ◽  
Transcriptional Level ◽  
Nuclear Factor ◽  
Activator Protein 1 ◽  
Activated T Cells ◽  
Dna Binding Activity ◽  
Interleukin 7 ◽  
Activated T Lymphocytes

Abstract In the present report, we studied the role of the stromal-derived cytokine interleukin-7 (IL-7) in the IL-2–gene regulation in activated T lymphocytes. Production of IL-2 requires the formation of transcription factors involved in the IL-2 –gene regulation. T-cell receptor (TCR)/CD3 engagement results in the activation of nuclear factor of activated T cells (NFAT), activator protein-1 (AP-1), and nuclear factor κB (NFκB), whereas the CD28 responsive complex (CD28RC) is activated in response to the CD28 signal. Costimulation of phytohemagglutinin/anti-CD28 activated T lymphocytes with IL-7 induces a fivefold enhanced IL-2–mRNA accumulation and a 2.5-fold enhanced protein secretion. The IL-2–gene transcription rate is increased 3.4-fold, indicating that the effect of IL-7 is in part mediated at the transcriptional level. The molecular mechanisms underlying the IL-7 effect involve the upregulation of the DNA binding activity of NFAT (60%) and AP-1 (120%), without affecting the activities of NFκB and CD28RC, which was confirmed by transfection assays. We also show that the IL-7–induced enhancement of the AP-1–DNA binding activity is not cyclosporin A-sensitive. Since AP-1 is part of the NFAT complex, we conclude that the IL-7–signaling pathway is involved in the activation of the fos and jun proteins of which AP-1 consists.

scholarly journals The DNA Binding Activity of C/EBP Transcription Factors Is Regulated in the G Phase of the Hepatocyte Cell Cycle

1995 ◽  
Vol 270 (30) ◽  
pp. 18123-18132 ◽  
Author(s):  
Basabi Rana ◽  
Yuhong Xie ◽  
David Mischoulon ◽  
Nancy L. R. Bucher ◽  
Stephen R. Farmer
Keyword(s):  
Cell Cycle ◽  
Transcription Factors ◽  
Dna Binding ◽  
Binding Activity ◽  
Dna Binding Activity
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