scholarly journals Cleavage of Bovine Collagen I by Neutrophil Collagenase MMP-8

2000 ◽  
Vol 275 (25) ◽  
pp. 18657-18663 ◽  
Author(s):  
Stefano Marini ◽  
Giovanni Francesco Fasciglione ◽  
Giampiero de Sanctis ◽  
Silvana D'Alessio ◽  
Vincenzo Politi ◽  
...  
Keyword(s):  
Collagen I ◽  
Bovine Collagen ◽  
Neutrophil Collagenase

scholarly journals Modulation of the Catalytic Activity of Neutrophil Collagenase MMP-8 on Bovine Collagen I

2002 ◽  
Vol 277 (26) ◽  
pp. 23123-23130 ◽  
Author(s):  
Magda Gioia ◽  
Giovanni Francesco Fasciglione ◽  
Stefano Marini ◽  
Silvana D'Alessio ◽  
Giampiero De Sanctis ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Collagen I ◽  
Bovine Collagen ◽  
Neutrophil Collagenase

scholarly journals Rapid Spheroid Assays in a 3-Dimensional Cell Culture Chip

2020 ◽  
Author(s):  
Jia Lin Teh ◽  
Siti Fairus Abdul Rahman ◽  
Gregory Domnic ◽  
Lengishwarra Satiyasilan ◽  
Nelson Jeng Yeou Chear ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Biology ◽  
Drug Sensitivity ◽  
3D Cell Culture ◽  
Collagen Matrix ◽  
High Volume ◽  
Collagen I ◽  
Bovine Collagen ◽  
High Concentrations ◽  
Drug Compound

Abstract Objective: The spheroid model provides a physiological platform to study cancer cell biology and drug sensitivity. Usage of bovine collagen I for spheroid assays is costly especially when experiments are conducted in 24-well plates, as high volume of bovine collagen I is needed. The aim of the study was to downsize spheroid assays to a microfluidic 3D cell culture chip and compare the growth, invasion and response to drug/compound of spheroids embedded in the 3D chip to spheroids embedded in 24-well plates.Results: Spheroids generated from nasopharyngeal carcinoma cell line HK-1 continuously grew and invaded into collagen matrix in a 24-well plate. Similar observations were noticed with spheroids embedded in the 3D chip. Large spheroids in both 24-well plate and the 3D chip disintegrated and invaded into the collagen matrix. Preliminary drug sensitivity assays showed that the growth and invasion of spheroids were inhibited when spheroids were treated with combination of cisplatin and paynantheine at high concentrations, in a 24-well plate. Comparable findings were obtained when spheroids were treated with the same drug combination in the 3D chip. Moving forward, spheroid assays could be performed in the 3D chip in a more high-throughput manner with minimal time and cost.

scholarly journals Characterization of the Mechanisms by which Gelatinase A, Neutrophil Collagenase, and Membrane-Type Metalloproteinase MMP-14 Recognize Collagen I and Enzymatically Process the Two α-Chains

2007 ◽  
Vol 368 (4) ◽  
pp. 1101-1113 ◽  
Author(s):  
Magda Gioia ◽  
Susanna Monaco ◽  
Giovanni Francesco Fasciglione ◽  
Anna Coletti ◽  
Andrea Modesti ◽  
...  
Keyword(s):  
Collagen I ◽  
Gelatinase A ◽  
Neutrophil Collagenase ◽  
Membrane Type

scholarly journals Rapid spheroid assays in a 3-dimensional cell culture chip

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jia Lin Teh ◽  
Siti Fairus Abdul Rahman ◽  
Gregory Domnic ◽  
Lengishwarra Satiyasilan ◽  
Nelson Jeng Yeou Chear ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Biology ◽  
Drug Sensitivity ◽  
3D Cell Culture ◽  
Collagen Matrix ◽  
High Volume ◽  
Collagen I ◽  
Bovine Collagen ◽  
High Concentrations ◽  
Drug Compound

Abstract Objective The spheroid model provides a physiological platform to study cancer cell biology and drug sensitivity. Usage of bovine collagen I for spheroid assays is costly especially when experiments are conducted in 24-well plates, as high volume of bovine collagen I is needed. The aim of the study was to downsize spheroid assays to a microfluidic 3D cell culture chip and compare the growth, invasion and response to drug/compound of spheroids embedded in the 3D chip to spheroids embedded in 24-well plates. Results Spheroids generated from nasopharyngeal carcinoma cell line HK-1 continuously grew and invaded into collagen matrix in a 24-well plate. Similar observations were noticed with spheroids embedded in the 3D chip. Large spheroids in both 24-well plate and the 3D chip disintegrated and invaded into the collagen matrix. Preliminary drug sensitivity assays showed that the growth and invasion of spheroids were inhibited when spheroids were treated with combination of cisplatin and paynantheine at high concentrations, in a 24-well plate. Comparable findings were obtained when spheroids were treated with the same drug combination in the 3D chip. Moving forward, spheroid assays could be performed in the 3D chip in a more high-throughput manner with minimal time and cost.

Thrombin Stimulated Platelet Accumulation on Protein Coated Glass Capillaries: Role of Adhesive Platelet α-Granule Proteins

1989 ◽  
Vol 62 (03) ◽  
pp. 989-995 ◽  
Author(s):  
Juliette N Mulvihill ◽  
J Andrew Davies ◽  
Florence Toti ◽  
Jean-Marie Freyssinet ◽  
Jean-Pierre Cazenave
Keyword(s):  
Human Platelets ◽  
Capillary Perfusion ◽  
Coated Glass ◽  
Bovine Collagen ◽  
Glass Capillaries ◽  
Human Thrombin ◽  
Platelet Accumulation ◽  
Granule Proteins ◽  
Von Willebrand

SummaryThe generation of trace amounts of thrombin at artificial surfaces in contact with blood is likely to be a contributing factor in thrombosis on biomaterials. Using an in vitro capillary perfusion system, platelet accumulation on glass surfaces, uncoated or precoated with purified bovine collagen or human plasma proteins, was determined in the presence or absence of preadsorbed purified human thrombin. Static adsorption for 15 min at 22° C from solutions of thrombin 100 NIH units (33 μg)/ml gave surface concentrations in the range 0.019-0.101 μg/cm2. Protein coated capillaries, thrombin treated or untreated, were perfused for 2 min at 37° C with suspensions of washed 111In-labeled human platelets in Tyrode's-albumin buffer containing 40% washed red blood cells, under conditions of controlled, non pulsatile laminar flow (50 s−1 or 2,000 s−1). Platelet accumulation was increased in the presence of surface adsorbed thrombin on uncoated and albumin or fibrinogen coated glass but little affected on fibronectin or collagen coated glass. On von Willebrand factor (vWF) coated glass, thrombin enhancement was observed only at high shear forces. In experiments using antibodies against human platelet α-granule proteins, thrombin stimulated platelet deposition in uncoated glass capillaries was inhibited at 2,000 s−1 by anti-vWF and to a lesser extent by anti-fibrinogen but not by antithrombospondin antibodies.

Fetal Bovine Collagen Grafts for Repair of Tegmen Defects and Encephaloceles via Middle Cranial Fossa Approach

2020 ◽  
Author(s):  
Anand Patel ◽  
Matthew R. Bartindale ◽  
Jehad A. Zakaria ◽  
Anand V. Germanwala ◽  
Douglas E. Anderson ◽  
...  
Keyword(s):  
Middle Cranial Fossa ◽  
Middle Cranial Fossa Approach ◽  
Bovine Collagen ◽  
Cranial Fossa ◽  
Fetal Bovine

Poldip2 Affects Collagen I Accumulation by Regulating the Expression of Zyxin in Vascular Smooth Muscle Cells

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 478-P
Author(s):  
MASAKAZU FUJII ◽  
NORIYUKI SONODA ◽  
MISATO OKAMOTO ◽  
HIDETAKA MORINAGA ◽  
YOSHIHIRO OGAWA ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Collagen I
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