scholarly journals Purification and Characterization of Chondroitin 4-Sulfotransferase from the Culture Medium of a Rat Chondrosarcoma Cell Line

1999 ◽  
Vol 274 (4) ◽  
pp. 2456-2463 ◽  
Author(s):  
Shinobu Yamauchi ◽  
Yukie Hirahara ◽  
Hiroaki Usui ◽  
Yoshiko Takeda ◽  
Megumi Hoshino ◽  
...  
Keyword(s):  
Cell Line ◽  
Culture Medium ◽  
Purification And Characterization ◽  
Chondrosarcoma Cell ◽  
Chondrosarcoma Cell Line

scholarly journals Establishment and Characterization of a Continuous Human Chondrosarcoma Cell Line, ch-2879: Comparative Histologic and Genetic Studies with Its Tumor of Origin

2003 ◽  
Vol 83 (6) ◽  
pp. 877-887 ◽  
Author(s):  
Rosario Gil-Benso ◽  
Concha Lopez-Gines ◽  
José Antonio López-Guerrero ◽  
Carmen Carda ◽  
Robert C Callaghan ◽  
...  
Keyword(s):  
Cell Line ◽  
Genetic Studies ◽  
Chondrosarcoma Cell ◽  
Human Chondrosarcoma ◽  
Chondrosarcoma Cell Line

Establishment and characterization of a novel dedifferentiated chondrosarcoma cell line, NCC-dCS1-C1

Human Cell ◽  
2019 ◽  
Vol 32 (2) ◽  
pp. 202-213
Author(s):  
Rieko Oyama ◽  
Fusako Kito ◽  
Mami Takahashi ◽  
Marimu Sakumoto ◽  
Kumiko Shiozawa ◽  
...  
Keyword(s):  
Cell Line ◽  
Dedifferentiated Chondrosarcoma ◽  
Chondrosarcoma Cell ◽  
Chondrosarcoma Cell Line

scholarly journals Characterization of Binding Properties of Urinary Trypsin Inhibitor to Cell-associated Binding Sites on Human Chondrosarcoma Cell Line HCS-2/8

2001 ◽  
Vol 276 (17) ◽  
pp. 13650-13656 ◽  
Author(s):  
Yasuyuki Hirashima ◽  
Hiroshi Kobayashi ◽  
Mika Suzuki ◽  
Yoshiko Tanaka ◽  
Naohiro Kanayama ◽  
...  
Keyword(s):  
Cell Line ◽  
Trypsin Inhibitor ◽  
Binding Sites ◽  
Urinary Trypsin Inhibitor ◽  
Binding Properties ◽  
Chondrosarcoma Cell ◽  
Human Chondrosarcoma ◽  
Chondrosarcoma Cell Line

Characterization of a newly established human chondrosarcoma cell line, CS-OKB

1998 ◽  
Vol 432 (6) ◽  
pp. 529-534 ◽  
Author(s):  
T. Chano ◽  
Hidetoshi Okabe ◽  
Yukikazu Saeki ◽  
Michihito Ishizawa ◽  
Keiji Matsumoto ◽  
...  
Keyword(s):  
Cell Line ◽  
Chondrosarcoma Cell ◽  
Human Chondrosarcoma ◽  
Chondrosarcoma Cell Line

scholarly journals Purification and characterization of class II histocompatibility antigens from a homozygous human B cell line.

1987 ◽  
Vol 262 (33) ◽  
pp. 16087-16094
Author(s):  
J C Gorga ◽  
V Horejsí ◽  
D R Johnson ◽  
R Raghupathy ◽  
J L Strominger
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Class Ii ◽  
Purification And Characterization ◽  
Histocompatibility Antigens ◽  
Human B Cell

scholarly journals Purification and characterization of adenosine deaminase from a genetically enriched mouse cell line.

1985 ◽  
Vol 260 (24) ◽  
pp. 13261-13267 ◽  
Author(s):  
D E Ingolia ◽  
C Y Yeung ◽  
I F Orengo ◽  
M L Harrison ◽  
E G Frayne ◽  
...  
Keyword(s):  
Cell Line ◽  
Adenosine Deaminase ◽  
Mouse Cell ◽  
Purification And Characterization ◽  
Mouse Cell Line

scholarly journals Partial purification and characterization of an endo-α-N-acetylgalactosaminidase from the culture medium of Streptomyces sp. OH-11242

1992 ◽  
Vol 288 (2) ◽  
pp. 475-482 ◽  
Author(s):  
I Ishii-Karakasa ◽  
H Iwase ◽  
K Hotta ◽  
Y Tanaka ◽  
S Omura
Keyword(s):  
Enzyme Activity ◽  
Culture Medium ◽  
Gel Filtration ◽  
Partial Purification ◽  
Gastric Mucus ◽  
Purification And Characterization ◽  
Streptomyces Sp ◽  
Optimum Ph ◽  
New Type

For the purification of a new type of endo-alpha-N-acetylgalactosaminidase from the culture medium of Streptomyces sp. OH-11242 (endo-GalNAc-ase-S) [Iwase, Ishii, Ishihara, Tanaka, Omura & Hotta (1988) Biochem. Biophys. Res. Commun. 151, 422-428], a method for assaying enzyme activity was established. Using purified pig gastric mucus glycoprotein (PGM) as the substrate, oligosaccharides liberated from PGM were pyridylaminated, and the reducing terminal sugars of oligosaccharides larger than Gal beta 1-3GalNAc were analysed by h.p.1.c. The crude enzyme of endo-GalNAc-ase-S was prepared as an 80% (w/v) ammonium sulphate precipitate from the concentrated culture medium. The enzyme was partially purified by gel chromatofocusing and subsequent DEAE-Toyopearl chromatography. Endo-enzyme activity eluted around pI 4.8 on a gel chromatofocusing column and eluted with 0.19-0.25 M-NaCl on a DEAE-Toyopearl column. In the enzyme fraction obtained, no exo-glycosidases or proteases could be detected. The molecular mass of the enzyme was estimated as 105 kDa by gel filtration, and the optimum pH was 5.5. Endo-GalNAc-ase-S hydrolysed the O-glycosidic linkage between GalNAc and Ser (Thr) in 3H-labelled and unlabelled asialofetuin, liberating both the disaccharide (Gal beta 1-3GalNAc) and the tetrasaccharide [Gal beta 1-3 (Gal beta 1-4GlcNAc beta 1-6)GalNAc]. When endo-alpha-N-acetylgalactosaminidase from Alcaligenes sp. (endo-GalNac-ase-A) was incubated with 3H-labelled and unlabelled asialofetuin, only the disaccharide (Gal beta 1-3GalNAc) was liberated.

scholarly journals A new human chondrosarcoma cell line (OUMS-27) that maintains chondrocytic differentiation

1998 ◽  
Vol 77 (6) ◽  
pp. 854-859 ◽  
Author(s):  
Toshiyuki Kunisada ◽  
Masahiro Miyazaki ◽  
Koichiro Mihara ◽  
Chong Gao ◽  
Akira Kawai ◽  
...  
Keyword(s):  
Cell Line ◽  
Chondrosarcoma Cell ◽  
Human Chondrosarcoma ◽  
Chondrocytic Differentiation ◽  
Chondrosarcoma Cell Line

scholarly journals GABAB receptor regulates proliferation in the high-grade chondrosarcoma cell line OUMS-27 via apoptotic pathways

BMC Cancer ◽  
2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Kiyoto Kanbara ◽  
Yoshinori Otsuki ◽  
Masahito Watanabe ◽  
Syunichi Yokoe ◽  
Yoshiaki Mori ◽  
...  
Keyword(s):  
Cell Line ◽  
Gabab Receptor ◽  
High Grade ◽  
Chondrosarcoma Cell ◽  
Apoptotic Pathways ◽  
Chondrosarcoma Cell Line
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