scholarly journals Single Cell Analysis and Selection of Living Retrovirus Vector-corrected Mucopolysaccharidosis VII Cells Using a Fluorescence-activated Cell Sorting-based Assay for Mammalian β-Glucuronidase Enzymatic Activity

1999 ◽  
Vol 274 (2) ◽  
pp. 657-665 ◽  
Author(s):  
Matthew C. Lorincz ◽  
Michael K. Parente ◽  
Mario Roederer ◽  
Garry P. Nolan ◽  
Zhenjun Diwu ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Single Cell ◽  
Cell Sorting ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Fluorescence Activated Cell Sorting ◽  
Retrovirus Vector ◽  
Selection Of

Hapten-Specific Single-Cell Selection of Hybridoma Clones by Fluorescence-Activated Cell Sorting for the Generation of Monoclonal Antibodies

2017 ◽  
Vol 89 (7) ◽  
pp. 4007-4012 ◽  
Author(s):  
Martin Dippong ◽  
Peter Carl ◽  
Christine Lenz ◽  
Jörg A. Schenk ◽  
Katrin Hoffmann ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Single Cell ◽  
Cell Sorting ◽  
Cell Selection ◽  
Fluorescence Activated Cell Sorting ◽  
Selection Of

scholarly journals Current techniques for single-cell lysis

2008 ◽  
Vol 5 (suppl_2) ◽  
Author(s):  
Robert B Brown ◽  
Julie Audet
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Laser Pulses ◽  
High Temporal Resolution ◽  
Cell Analysis ◽  
Careful Selection ◽  
Analysis Techniques ◽  
Cell Lysate ◽  
Selection Of

Owing to the small quantities of analytes and small volumes involved in single-cell analysis techniques, manipulation strategies must be chosen carefully. The lysis of single cells for downstream chemical analysis in capillaries and lab-on-a-chip devices can be achieved by optical, acoustic, mechanical, electrical or chemical means, each having their respective strengths and weaknesses. Selection of the most appropriate lysis method will depend on the particulars of the downstream cell lysate processing. Ultrafast lysis techniques such as the use of highly focused laser pulses or pulses of high voltage are suitable for applications requiring high temporal resolution. Other factors, such as whether the cells are adherent or in suspension and whether the proteins to be collected are desired to be native or denatured, will determine the suitability of detergent-based lysis methods. Therefore, careful selection of the proper lysis technique is essential for gathering accurate data from single cells.

New Micro Devices for Single Cell Analysis, Cell Sorting and Cloning-on-a-Chip: The CytoconTM Instrument

2000 ◽  
pp. 443-446 ◽  
Author(s):  
G. Gradl ◽  
T. Müller ◽  
A. Pfennig ◽  
S. Shirley ◽  
T. Schnelle ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Sorting ◽  
Single Cell Analysis ◽  
Cell Analysis

scholarly journals Cyto-Mine: An Integrated, Picodroplet System for High-Throughput Single-Cell Analysis, Sorting, Dispensing, and Monoclonality Assurance

2020 ◽  
Vol 25 (2) ◽  
pp. 177-189 ◽  
Author(s):  
Dimitris Josephides ◽  
Serena Davoli ◽  
William Whitley ◽  
Raphael Ruis ◽  
Robert Salter ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Line ◽  
High Throughput ◽  
Cell Sorting ◽  
High Throughput Screening ◽  
Single Cell Analysis ◽  
Microtiter Plate ◽  
Cell Analysis ◽  
Fully Integrated ◽  
Clonal Cell Lines

The primary goal of bioprocess cell line development is to obtain high product yields from robustly growing and well-defined clonal cell lines in timelines measured in weeks rather than months. Likewise, high-throughput screening of B cells and hybridomas is required for most cell line engineering workflows. A substantial bottleneck in these processes is detecting and isolating rare clonal cells with the required characteristics. Traditionally, this was achieved by the resource-intensive method of limiting dilution cloning, and more recently aided by semiautomated technologies such as cell sorting (e.g., fluorescence-activated cell sorting) and colony picking. In this paper we report on our novel Cyto-Mine Single Cell Analysis and Monoclonality Assurance System, which overcomes the limitations of current technologies by screening hundreds of thousands of individual cells for secreted target proteins, and then isolating and dispensing the highest producers into microtiter plate wells (MTP). The Cyto-Mine system performs this workflow using a fully integrated, microfluidic Cyto-Cartridge. Critically, all reagents and Cyto-Cartridges used are animal component-free (ACF) and sterile, thus allowing fast, robust, and safe isolation of desired cells.

scholarly journals Towards high-throughput microfluidic Raman-activated cell sorting

The Analyst ◽  
2015 ◽  
Vol 140 (18) ◽  
pp. 6163-6174 ◽  
Author(s):  
Qiang Zhang ◽  
Peiran Zhang ◽  
Honglei Gou ◽  
Chunbo Mou ◽  
Wei E. Huang ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Cell Sorting ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Label Free ◽  
Non Invasive

Raman-activated cell sorting (RACS) is a promising single-cell analysis technology that is able to identify and isolate individual cells of targeted type, state or environment from an isogenic population or complex consortium of cells, in a label-free and non-invasive manner.

Author response for "Immunocyte single cell analysis of vaccine‐induced narcolepsy"

2020 ◽  
Author(s):  
Alexander Lind ◽  
Falastin Salami ◽  
Anne‐Marie Landtblom ◽  
Lars Palm ◽  
Åke Lernmark ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Author Response ◽  
Cell Analysis
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