scholarly journals Pervanadate Stimulates Amylase Release and Protein Tyrosine Phosphorylation of Paxillin and p125FAKin Differentiated AR4-2J Pancreatic Acinar Cells

1998 ◽  
Vol 273 (26) ◽  
pp. 16366-16373 ◽  
Author(s):  
Peter Feick ◽  
Sven Gilhaus ◽  
Irene Schulz
Keyword(s):  
Tyrosine Phosphorylation ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Protein Tyrosine Phosphorylation ◽  
Amylase Release ◽  
Protein Tyrosine

Tyrphostins inhibit secretagogue-induced 1,4,5-IP3 production and amylase release in pancreatic acini

1994 ◽  
Vol 266 (3) ◽  
pp. G363-G371
Author(s):  
A. Piiper ◽  
D. Stryjek-Kaminska ◽  
J. Stein ◽  
W. F. Caspary ◽  
S. Zeuzem
Keyword(s):  
Tyrosine Phosphorylation ◽  
Phosphorylation Site ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Amylase Secretion ◽  
Specific Cell ◽  
Amylase Release ◽  
Pancreatic Acini ◽  
Cholecystokinin Octapeptide ◽  
Permeabilized Cells

We examined the role of protein tyrosine kinase inhibitors (tyrphostins) in secretagogue-induced inositol 1,4,5-trisphosphate (1,4,5-IP3) production and amylase secretion in rat pancreatic acinar cells. The data show that various specific cell-permeant tyrphostins (methyl 2,5-dihydroxycinnamate, tyrphostin 25, and genistein) inhibited the cholecystokinin octapeptide-, carbachol-, and bombesin-induced 1,4,5-IP3 production and amylase release. In digitonin-permeabilized cells, tyrphostins decreased 1,4,5-IP3 accumulation and amylase release generated by directly stimulating G proteins with the weakly hydrolyzable GTP analogue guanosine 5'-O-(3-thiotriphosphate). Tyrphostins had no effect on vasoactive intestinal peptide-induced amylase secretion. In isolated pancreatic acinar membranes, cholecystokinin octapeptide caused a rapid increase in tyrosine phosphorylation of a synthetic peptide containing the 12-amino acid sequence around a tyrosine phosphorylation site in pp6osrc. These results provide evidence that tyrosine kinases are involved in the activation of phospholipase C by G protein-coupled receptors in pancreatic acinar cells.

scholarly journals Angiotensin II-induced protein tyrosine phosphorylation in neonatal rat cardiac fibroblasts.

1994 ◽  
Vol 269 (30) ◽  
pp. 19626-19632
Author(s):  
W. Schorb ◽  
T.C. Peeler ◽  
N.N. Madigan ◽  
K.M. Conrad ◽  
K.M. Baker
Keyword(s):  
Angiotensin Ii ◽  
Tyrosine Phosphorylation ◽  
Cardiac Fibroblasts ◽  
Neonatal Rat ◽  
Protein Tyrosine Phosphorylation ◽  
Protein Tyrosine
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