scholarly journals A Steady-state Template Model That Describes the Kinetics of Fibrin-stimulated [Glu1]- and [Lys78]Plasminogen Activation by Native tissue-type Plasminogen Activator and Variants That Lack Either the Finger or Kringle-2 Domain

1996 ◽  
Vol 272 (4) ◽  
pp. 2183-2191 ◽  
Author(s):  
Anton J. G. Horrevoets ◽  
Hans Pannekoek ◽  
Michael E. Nesheim
Keyword(s):  
Steady State ◽  
Plasminogen Activator ◽  
Tissue Type ◽  
Plasminogen Activation ◽  
Tissue Type Plasminogen Activator ◽  
Template Model ◽  
Native Tissue ◽  
Type Plasminogen Activator ◽  
Kringle 2 ◽  
Kinetics Of

scholarly journals Localization in the fibrinogen γ-chain of a new site that is involved in the acceleration of the tissue-type plasminogen activator-catalysed activation of plasminogen

1992 ◽  
Vol 283 (1) ◽  
pp. 187-191 ◽  
Author(s):  
O Yonekawa ◽  
M Voskuilen ◽  
W Nieuwenhuizen
Keyword(s):  
Plasminogen Activator ◽  
Sequence Data ◽  
Disulphide Bond ◽  
Void Volume ◽  
Tissue Type ◽  
Plasminogen Activation ◽  
Main Band ◽  
Tissue Type Plasminogen Activator ◽  
Type Plasminogen Activator ◽  
Water Ph

In previous publications [e.g. Voskuilen, Vermond, Veeneman, Van Boom, Klasen, Zegers & Nieuwenhuizen (1987) J. Biol. Chem. 262, 5944-5946] we have shown that fibrin(ogen) chain fragment A alpha-(148-160) contains a site that contributes to the acceleration of Glu-plasminogen activation by tissue-type plasminogen activator (t-PA). In contrast with fibrin, this peptide, however, does not enhance the rate of mini-plasminogen activation. Therefore, possibly more stimulatory sites than A alpha-(148-160) are present in fibrin. In the present investigation we have localized a possible second type of stimulatory site in the fibrin(ogen) molecule. A whole CNBr digest of fibrinogen was applied to a Bio-Gel P-2 column run in water, pH 4. Two peaks with stimulatory activity were observed, one at the void volume and one between the void volume and the total volume. The former contained the previously described stimulating fragment FCB-2 [which comprises A alpha-(148-160)]; the latter had not been observed before and was characterized further. The stimulating material in the low-M(r) fraction of the Bio-Gel P-2 column was precipitated at pH 8.3 in a virtually pure form. It has a high tryptophan content, and an M(r) of 6500 as assessed by SDS/PAGE. On reduction, a main band of M(r) 2500 is seen, plus a weakly staining band of M(r) 4000. These properties plus the amino acid sequence data identify the fragment as FCB-5. FCB-5 consists of two chains, i.e. gamma-(311-336) and gamma-(337-379), linked by a single disulphide bond between Cys-gamma-326 and Cys-gamma-339. Both these chains and the disulphide bond appear to be essential for rate enhancement. FCB-5 enhances the activation rates of Glu-, mini- and micro-plasminogen, with all five kringles, only kringle V and without kringles respectively. FCB-5 binds t-PA, but none of the plasminogen forms binds to FCB-5. This indicates that the rate enhancements induced by FCB-5 are due to an effect on t-PA.

The Thrombolytic Effects of Native Tissue-Type Plasminogen Activator (AK-124) on Experimental Canine Coronary Thrombosis

Angiology ◽  
1989 ◽  
Vol 40 (5) ◽  
pp. 429-435 ◽  
Author(s):  
Takanobu Tomaru ◽  
Yasumi Uchida ◽  
Hiroyuki Sonoki ◽  
Masami Tsukamoto ◽  
Tsuneaki Sugimoto
Keyword(s):  
Plasminogen Activator ◽  
Coronary Thrombosis ◽  
Tissue Type ◽  
Tissue Type Plasminogen Activator ◽  
Native Tissue ◽  
Type Plasminogen Activator ◽  
Experimental Canine

Ligand Binding to the Tissue-Type Plasminogen Activator Kringle 2 Domain: Structural Characterization by 1H-NMR

Biochemistry ◽  
1995 ◽  
Vol 34 (9) ◽  
pp. 2739-2750 ◽  
Author(s):  
In-Ja L. Byeon ◽  
Robert F. Kelley ◽  
Michael G. Mulkerrin ◽  
Seong Soo A. An ◽  
Miguel Llinas
Keyword(s):  
Ligand Binding ◽  
Plasminogen Activator ◽  
1H Nmr ◽  
Structural Characterization ◽  
Tissue Type ◽  
Tissue Type Plasminogen Activator ◽  
Type Plasminogen Activator ◽  
Kringle 2
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