scholarly journals Polymerase Chain Reaction-based Cloning of Alkyl-dihydroxyacetonephosphate Synthase Complementary DNA from Guinea Pig Liver

1997 ◽  
Vol 272 (2) ◽  
pp. 798-803 ◽  
Author(s):  
Edwin C. J. M. de Vet ◽  
Anna W. M. Zomer ◽  
Gaston J. H. T. J. Lahaut ◽  
Henk van den Bosch
Keyword(s):  
Polymerase Chain Reaction ◽  
Guinea Pig ◽  
Chain Reaction ◽  
Pig Liver ◽  
Complementary Dna ◽  
Polymerase Chain ◽  
Guinea Pig Liver

cDNA Sequencing of Guinea Pig a2-HS Glycoprotein, Its Expression in Various Tissues and Acute Phase Expression

1999 ◽  
Vol 380 (1) ◽  
pp. 95-99 ◽  
Author(s):  
K. Yoshida ◽  
Y. Suzuki ◽  
K. Yamamoto ◽  
H. Sinohara
Keyword(s):  
Skeletal Muscle ◽  
Guinea Pig ◽  
Mrna Levels ◽  
Cdna Sequencing ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pig Liver ◽  
Polymerase Chain ◽  
Rapid Amplification ◽  
Guinea Pig Liver

Abstract cDNA encoding α2-HS glycoprotein was amplified from guinea pig liver mRNA by reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends, cloned and sequenced. By RTPCR and nested PCR, α2-HS glycoprotein mRNA was detected not only in liver tissue but also in pancreas, stomach, small intestine, colon, spleen, kidney, testis, skeletal muscle, brain, heart and leukocytes, but not in the lung. The α2-HS glycoprotein mRNA levels in the liver were reduced to half at 48 h after subcutaneous injection of turpentine oil.

scholarly journals Assessment of Aspergillus fumigatus in Guinea Pig Bronchoalveolar Lavages and Pulmonary Tissue by Culture and Realtime Polymerase Chain Reaction Studies

2012 ◽  
Vol 13 (1) ◽  
pp. 726-736
Author(s):  
Dennis G. Hooper ◽  
Vincent E. Bolton ◽  
John S. Sutton ◽  
Frederick T. Guilford ◽  
David C. Straus ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Guinea Pig ◽  
Aspergillus Fumigatus ◽  
Chain Reaction ◽  
Pulmonary Tissue ◽  
Polymerase Chain

scholarly journals Point mutations in the uroporphyrinogen III synthase gene in congenital erythropoietic porphyria (Gunther's disease)

Blood ◽  
1990 ◽  
Vol 75 (9) ◽  
pp. 1763-1765 ◽  
Author(s):  
JC Deybach ◽  
H de Verneuil ◽  
S Boulechfar ◽  
B Grandchamp ◽  
Y Nordmann
Keyword(s):  
Polymerase Chain Reaction ◽  
Autosomal Recessive ◽  
Distinct Point ◽  
Point Mutations ◽  
Chain Reaction ◽  
Complementary Dna ◽  
Congenital Erythropoietic Porphyria ◽  
Molecular Abnormality ◽  
Allele Specific ◽  
Polymerase Chain

Congenital erythropoietic porphyria (Gunther's disease) is a rare disorder of heme biosynthesis inherited in an autosomal recessive fashion. The molecular abnormality responsible for the characteristic defect in uroporphyrinogen III synthase activity was investigated in two patients. For the first patient, complementary DNA was specifically amplified using the polymerase chain reaction and subsequently cloned and sequenced. Data obtained revealed the coexistence of two distinct point mutations: a T to C change in codon 73 (arginine in place of a cysteine) and a C to T change in codon 53 (leucine in place of a proline). The second case was studied by hybridization with allele specific oligonucleotides and was found to be homozygous for the same mutation in codon 53. These are the first mutations to be recognized in the uroporphyrinogen III synthase gene from congenital erythropoietic porphyria patients.

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