scholarly journals Activation of a High Affinity G Protein-coupled Plasma Membrane Receptor by Sphingosine-1-phosphate

1996 ◽  
Vol 271 (4) ◽  
pp. 2082-2087 ◽  
Author(s):  
Chris J. van Koppen ◽  
Dagmar Meyer zu Heringdorf ◽  
Kai T. Laser ◽  
Chunyi Zhang ◽  
Karl H. Jakobs ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
G Protein ◽  
Membrane Receptor ◽  
High Affinity ◽  
Sphingosine 1 Phosphate ◽  
Plasma Membrane Receptor ◽  
G Protein Coupled

scholarly journals Comment on "A G Protein Coupled Receptor Is a Plasma Membrane Receptor for the Plant Hormone Abscisic Acid"

Science ◽  
2007 ◽  
Vol 318 (5852) ◽  
pp. 914c-914c ◽  
Author(s):  
C. A. Johnston ◽  
B. R. Temple ◽  
J.-G. Chen ◽  
Y. Gao ◽  
E. N. Moriyama ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Abscisic Acid ◽  
G Protein ◽  
Plant Hormone ◽  
Membrane Receptor ◽  
G Protein Coupled Receptor ◽  
Plasma Membrane Receptor ◽  
G Protein Coupled

A G Protein-Coupled Receptor Is a Plasma Membrane Receptor for the Plant Hormone Abscisic Acid

Science ◽  
2007 ◽  
Vol 315 (5819) ◽  
pp. 1712-1716 ◽  
Author(s):  
X. Liu ◽  
Y. Yue ◽  
B. Li ◽  
Y. Nie ◽  
W. Li ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Abscisic Acid ◽  
G Protein ◽  
Plant Hormone ◽  
Membrane Receptor ◽  
G Protein Coupled Receptor ◽  
Plasma Membrane Receptor ◽  
G Protein Coupled

Sphingosine-1-phosphate is a ligand for the G protein-coupled receptor EDG-6

Blood ◽  
2000 ◽  
Vol 95 (8) ◽  
pp. 2624-2629 ◽  
Author(s):  
James R. Van Brocklyn ◽  
Markus H. Gräler ◽  
Günter Bernhardt ◽  
John P. Hobson ◽  
Martin Lipp ◽  
...  
Keyword(s):  
G Protein ◽  
Hek293 Cells ◽  
Hematopoietic Tissue ◽  
G Protein Coupled Receptor ◽  
High Affinity ◽  
Extracellular Signal ◽  
Sphingosine 1 Phosphate ◽  
High Affinity Receptor ◽  
High Concentrations ◽  
G Protein Coupled

Abstract EDG-6 is a recently cloned member of the endothelial differentiation gene (EDG) G protein-coupled receptor family that is expressed in lymphoid and hematopoietic tissue and in the lung. Homology of EDG-6 to the known sphingosine-1-phosphate (SPP) receptors EDG-1, EDG-3, and EDG-5 and lysophosphatidic acid (LPA) receptors EDG-2 and EDG-4 suggested that its ligand may be a lysophospholipid or lysosphingolipid. We examined the binding of [32P]SPP to HEK293 cells, transiently transfected with cDNA encoding EDG-6. Binding of [32P]SPP was saturable, demonstrating high affinity (KD = 63 nmol/L). Binding was also specific for SPP, as only unlabeled SPP and sphinganine-1-phosphate, which lacks the trans double bond at the 4 position, potently displaced radiolabeled SPP. LPA did not compete for binding of SPP at any concentration tested, whereas sphingosylphosphorylcholine competed for binding to EDG-6, but only at very high concentrations. In addition, SPP activated extracellular signal-regulated kinase (Erk) in EDG-6 transfected cells in a pertussis toxin-sensitive manner. These results indicate that EDG-6 is a high affinity receptor for SPP, which couples to a Gi/o protein, resulting in the activation of growth-related signaling pathways.

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