scholarly journals Identification of a gene, spoIIR, that links the activation of sigma E to the transcriptional activity of sigma F during sporulation in Bacillus subtilis.

1995 ◽  
Vol 92 (6) ◽  
pp. 2012-2016 ◽  
Author(s):  
M. L. Karow ◽  
P. Glaser ◽  
P. J. Piggot
Keyword(s):  
Bacillus Subtilis ◽  
Transcriptional Activity ◽  
Sigma E

scholarly journals The Bacillus subtilis spoIIG operon encodes both sigma E and a gene necessary for sigma E activation.

1988 ◽  
Vol 170 (2) ◽  
pp. 507-511 ◽  
Author(s):  
R M Jonas ◽  
E A Weaver ◽  
T J Kenney ◽  
C P Moran ◽  
W G Haldenwang
Keyword(s):  
Bacillus Subtilis ◽  
Sigma E

scholarly journals Bacillus subtilis spoVE gene is transcribed by sigma E-associated RNA polymerase.

1993 ◽  
Vol 175 (13) ◽  
pp. 4081-4086 ◽  
Author(s):  
A Miyao ◽  
G Theeragool ◽  
M Takeuchi ◽  
Y Kobayashi
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Sigma E

scholarly journals ςK Can Negatively RegulatesigE Expression by Two Different Mechanisms during Sporulation of Bacillus subtilis

1999 ◽  
Vol 181 (13) ◽  
pp. 4081-4088 ◽  
Author(s):  
Bin Zhang ◽  
Paolo Struffi ◽  
Lee Kroos
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Sigma Factor ◽  
Mother Cell ◽  
Transcriptional Activity ◽  
The Other ◽  
Mutant Form ◽  
Gene Encoding ◽  
Negative Effect ◽  
Cell Gene Expression

ABSTRACT Temporal and spatial gene regulation during Bacillus subtilis sporulation involves the activation and inactivation of multiple sigma subunits of RNA polymerase in a cascade. In the mother cell compartment of sporulating cells, expression of thesigE gene, encoding the earlier-acting sigma factor, ςE, is negatively regulated by the later-acting sigma factor, ςK. Here, it is shown that the negative feedback loop does not require SinR, an inhibitor of sigEtranscription. Production of ςK about 1 h earlier than normal does affect Spo0A, which when phosphorylated is an activator of sigE transcription. A mutation in thespo0A gene, which bypasses the phosphorelay leading to the phosphorylation of Spo0A, diminished the negative effect of early ςK production on sigE expression early in sporulation. Also, early production of ςK reduced expression of other Spo0A-dependent genes but not expression of the Spo0A-independent ald gene. In contrast, bothsigE and ald were overexpressed late in development of cells that fail to make ςK. Theald promoter, like the sigE promoter, is believed to be recognized by ςA RNA polymerase, suggesting that ςK may inhibit ςA activity late in sporulation. To exert this negative effect, ςKmust be transcriptionally active. A mutant form of ςKthat associates with core RNA polymerase, but does not direct transcription of a ςK-dependent gene, failed to negatively regulate expression of sigE or aldlate in development. On the other hand, the negative effect of early ςK production on sigE expression early in sporulation did not require transcriptional activity of ςK RNA polymerase. These results demonstrate that ςK can negatively regulate sigE expression by two different mechanisms, one observed when ςK is produced earlier than normal, which does not require ςKto be transcriptionally active and affects Spo0A, and the other observed when ςK is produced at the normal time, which requires ςK RNA polymerase transcriptional activity. The latter mechanism facilitates the switch from ςE to ςK in the cascade controlling mother cell gene expression.

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